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Immobilized pH gradients : theory and methodology /

Immobilized pH gradients (IPG) represent the most advanced development of isoelectric focusing (IEF). Originally developed to overcome all the problems of IEF in soluble amphoteric buffers (CA) (such as pH gradient instability, complexation with CA chemicals, unreproducibility of pH gradients, prote...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: Righetti, P. G. (Autor)
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Amsterdam ; New York : Elsevier, 1990.
Colección:Laboratory techniques in biochemistry and molecular biology ; v. 20.
Temas:
Acceso en línea:Texto completo
Texto completo

MARC

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100 1 |a Righetti, P. G.,  |e author. 
245 1 0 |a Immobilized pH gradients :  |b theory and methodology /  |c Pier Giorgie Righetti. 
260 |a Amsterdam ;  |a New York :  |b Elsevier,  |c 1990. 
300 |a 1 online resource (xv, 397 pages) :  |b illustrations 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
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490 1 |a Laboratory techniques in biochemistry and molecular biology ;  |v v. 20 
504 |a Includes bibliographical references and index. 
505 0 |a Front Cover; IMMOBILIZED pH GRADIENTS: THEORY AND METHODOLOGY; Copyright Page; Contents; List of abbreviations; Acknowledgments; Introduction; Chapter 1. The chemicals; Chapter 2. Theory; Chapter 3. Analytical IPGs; Chapter 4. Two-dimensional (2-D) maps; Chapter 5. Preparative aspects of immobilized pH gradients; Chapter 6. Some applications of IPGs; Chapter 7. Post scriptum: Maestro Volta; References; Subject Index. 
520 |a Immobilized pH gradients (IPG) represent the most advanced development of isoelectric focusing (IEF). Originally developed to overcome all the problems of IEF in soluble amphoteric buffers (CA) (such as pH gradient instability, complexation with CA chemicals, unreproducibility of pH gradients, protein precipitation at the pI), it turned out to be an entirely new technique, quite different in principle and operation from conventional IEF. The book is thus meant to bring the reader up to date with this fast developing field. The book is divided into six chapters containing information on: detail. 
506 |3 Use copy  |f Restrictions unspecified  |2 star  |5 MiAaHDL 
533 |a Electronic reproduction.  |b [Place of publication not identified] :  |c HathiTrust Digital Library,  |d 2011.  |5 MiAaHDL 
538 |a Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.  |u http://purl.oclc.org/DLF/benchrepro0212  |5 MiAaHDL 
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588 0 |a Print version record. 
650 0 |a Isoelectric focusing. 
650 0 |a Hydrogen-ion concentration. 
650 2 |a Isoelectric Focusing  |0 (DNLM)D007525 
650 2 |a Hydrogen-Ion Concentration  |0 (DNLM)D006863 
650 6 |a Focalisation iso�electrique.  |0 (CaQQLa)201-0065201 
650 6 |a pH.  |0 (CaQQLa)201-0051969 
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650 7 |a PH.  |2 fmesh 
650 7 |a pH  |x Analyse.  |2 ram 
776 0 8 |i Print version:  |a Righetti, P.G.  |t Immobilized pH gradients.  |d Amsterdam ; New York : Elsevier, 1990  |z 0444813012  |z 9780444813015  |w (DLC) 89071525  |w (OCoLC)20932929 
830 0 |a Laboratory techniques in biochemistry and molecular biology ;  |v v. 20. 
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