Immobilized pH gradients : theory and methodology /
Immobilized pH gradients (IPG) represent the most advanced development of isoelectric focusing (IEF). Originally developed to overcome all the problems of IEF in soluble amphoteric buffers (CA) (such as pH gradient instability, complexation with CA chemicals, unreproducibility of pH gradients, prote...
Clasificación: | Libro Electrónico |
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Autor principal: | |
Formato: | Electrónico eBook |
Idioma: | Inglés |
Publicado: |
Amsterdam ; New York :
Elsevier,
1990.
|
Colección: | Laboratory techniques in biochemistry and molecular biology ;
v. 20. |
Temas: | |
Acceso en línea: | Texto completo Texto completo |
MARC
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042 | |a dlr | ||
050 | 4 | |a QP519 |b .L2eb vol. 20 | |
070 | |a QD415.5.W6 |b v.20 | ||
072 | 7 | |a SCI |x 007000 |2 bisacsh | |
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100 | 1 | |a Righetti, P. G., |e author. | |
245 | 1 | 0 | |a Immobilized pH gradients : |b theory and methodology / |c Pier Giorgie Righetti. |
260 | |a Amsterdam ; |a New York : |b Elsevier, |c 1990. | ||
300 | |a 1 online resource (xv, 397 pages) : |b illustrations | ||
336 | |a text |b txt |2 rdacontent | ||
337 | |a computer |b c |2 rdamedia | ||
338 | |a online resource |b cr |2 rdacarrier | ||
490 | 1 | |a Laboratory techniques in biochemistry and molecular biology ; |v v. 20 | |
504 | |a Includes bibliographical references and index. | ||
505 | 0 | |a Front Cover; IMMOBILIZED pH GRADIENTS: THEORY AND METHODOLOGY; Copyright Page; Contents; List of abbreviations; Acknowledgments; Introduction; Chapter 1. The chemicals; Chapter 2. Theory; Chapter 3. Analytical IPGs; Chapter 4. Two-dimensional (2-D) maps; Chapter 5. Preparative aspects of immobilized pH gradients; Chapter 6. Some applications of IPGs; Chapter 7. Post scriptum: Maestro Volta; References; Subject Index. | |
520 | |a Immobilized pH gradients (IPG) represent the most advanced development of isoelectric focusing (IEF). Originally developed to overcome all the problems of IEF in soluble amphoteric buffers (CA) (such as pH gradient instability, complexation with CA chemicals, unreproducibility of pH gradients, protein precipitation at the pI), it turned out to be an entirely new technique, quite different in principle and operation from conventional IEF. The book is thus meant to bring the reader up to date with this fast developing field. The book is divided into six chapters containing information on: detail. | ||
506 | |3 Use copy |f Restrictions unspecified |2 star |5 MiAaHDL | ||
533 | |a Electronic reproduction. |b [Place of publication not identified] : |c HathiTrust Digital Library, |d 2011. |5 MiAaHDL | ||
538 | |a Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. |u http://purl.oclc.org/DLF/benchrepro0212 |5 MiAaHDL | ||
583 | 1 | |a digitized |c 2011 |h HathiTrust Digital Library |l committed to preserve |2 pda |5 MiAaHDL | |
588 | 0 | |a Print version record. | |
650 | 0 | |a Isoelectric focusing. | |
650 | 0 | |a Hydrogen-ion concentration. | |
650 | 2 | |a Isoelectric Focusing |0 (DNLM)D007525 | |
650 | 2 | |a Hydrogen-Ion Concentration |0 (DNLM)D006863 | |
650 | 6 | |a Focalisation iso�electrique. |0 (CaQQLa)201-0065201 | |
650 | 6 | |a pH. |0 (CaQQLa)201-0051969 | |
650 | 7 | |a SCIENCE |x Life Sciences |x Biochemistry. |2 bisacsh | |
650 | 7 | |a Hydrogen-ion concentration. |2 fast |0 (OCoLC)fst00965064 | |
650 | 7 | |a Isoelectric focusing. |2 fast |0 (OCoLC)fst00980154 | |
650 | 7 | |a Focalisation iso�electrique. |2 fmesh | |
650 | 7 | |a PH. |2 fmesh | |
650 | 7 | |a pH |x Analyse. |2 ram | |
776 | 0 | 8 | |i Print version: |a Righetti, P.G. |t Immobilized pH gradients. |d Amsterdam ; New York : Elsevier, 1990 |z 0444813012 |z 9780444813015 |w (DLC) 89071525 |w (OCoLC)20932929 |
830 | 0 | |a Laboratory techniques in biochemistry and molecular biology ; |v v. 20. | |
856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780444813015 |z Texto completo |
856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00757535/20 |z Texto completo |