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Cellular and molecular procedures in developmental biology /

This volume incorporates practical methods at the level of molecular, cellular, and whole organism biology in vertebrate and invertebrate models. It presents straightforward protocols written step-by-step for state-of-the-art techniques with the emphasis on single-cell resolution procedures. Key Fea...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: De Pablo, Flora (Editor ), Ferr�us, Alberto (Editor ), Stern, C. D. (Claudio D.) (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego : Academic Press, �1998.
Colección:Current topics in developmental biology ; v. 36.
Temas:
Acceso en línea:Texto completo
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Tabla de Contenidos:
  • Avian embryo as a model in developmental studies : chimeras and in vitro clonal analysis / Elisabeth Dupin, Catherine Ziller, and Nicole M. Le Douarin
  • Inhibition of gene expression by antisense oligonucleotides in chick embryos in vitro and in vivo / Aixa V. Morales and Flora de Pablo
  • Lineage analysis using retroviral vectors / Constance L. Cepko [and others]
  • Use of dominant negative constructs to modulate gene expression / Giorgio Lagna and Ali Hemmati-Brivanlou
  • Use of embryonic stem cells for the genetic manipulation of the mouse / Miguel Torres
  • Organoculture of otic vesicle and ganglion / Juan J. Garrido [and others]
  • Organcoculture of the chick embryonic neuroretina / Enrique J. de la Rosa, Begona Diaz, and Flora de Pablo
  • Embryonic explant and slice preparations for studies of cell migration and axon guidance / Catherine E. Krull and Paul M. Kulesa
  • Culture of avian sympathetic neurons / Alexander V. Holst and Hermann Roher
  • Analysis of gene expression in cultured primary neurons / Ming-Ji Fann and Paul H. Patterson
  • Selective aggregation assays for embryonic brain cells and cell lines / Shinichi Nakagawa, Hiroaki Matsunami, and Masatoshi Takeichi
  • Flow cytometric analysis of whole organs and embryos / Jose Serna, Belen Pimentel, and Enrique J. de la Rosa
  • Detection of multiple gene products simulaneously by in situ hybridization and immunohistochemistry in whole mounts of avian embryos / Claudio D. Stern
  • Cloning of genes from single neurons / Catherine Dulac
  • Methods for detecting and quantifying apoptosis / Nicola J. McCarthy and Gerard I. Evan
  • Methods in drosophila cell cycle biology / Fabian Feiguin, Salud Llamazares, and Cayetano Gonzalez
  • Single central nervous system neurons in culture / Juan Lerma, Miquel Morales, and Maria de los Angeles Vicente
  • Patch-clamp recordings from drosophila presynaptic terminals / Manuel Martinez-Padron and Alberto Ferrus.