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Cellular and molecular procedures in developmental biology /

This volume incorporates practical methods at the level of molecular, cellular, and whole organism biology in vertebrate and invertebrate models. It presents straightforward protocols written step-by-step for state-of-the-art techniques with the emphasis on single-cell resolution procedures. Key Fea...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: De Pablo, Flora (Editor ), Ferr�us, Alberto (Editor ), Stern, C. D. (Claudio D.) (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego : Academic Press, �1998.
Colección:Current topics in developmental biology ; v. 36.
Temas:
Acceso en línea:Texto completo
Texto completo

MARC

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245 0 0 |a Cellular and molecular procedures in developmental biology /  |c edited by Flora de Pablo, Alberto Ferr�us, Claudio D. Stern. 
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490 1 |a Current topics in developmental biology ;  |v v. 36 
504 |a Includes bibliographical references and index. 
505 0 |a Avian embryo as a model in developmental studies : chimeras and in vitro clonal analysis / Elisabeth Dupin, Catherine Ziller, and Nicole M. Le Douarin -- Inhibition of gene expression by antisense oligonucleotides in chick embryos in vitro and in vivo / Aixa V. Morales and Flora de Pablo -- Lineage analysis using retroviral vectors / Constance L. Cepko [and others] -- Use of dominant negative constructs to modulate gene expression / Giorgio Lagna and Ali Hemmati-Brivanlou -- Use of embryonic stem cells for the genetic manipulation of the mouse / Miguel Torres -- Organoculture of otic vesicle and ganglion / Juan J. Garrido [and others] -- Organcoculture of the chick embryonic neuroretina / Enrique J. de la Rosa, Begona Diaz, and Flora de Pablo -- Embryonic explant and slice preparations for studies of cell migration and axon guidance / Catherine E. Krull and Paul M. Kulesa -- Culture of avian sympathetic neurons / Alexander V. Holst and Hermann Roher -- Analysis of gene expression in cultured primary neurons / Ming-Ji Fann and Paul H. Patterson -- Selective aggregation assays for embryonic brain cells and cell lines / Shinichi Nakagawa, Hiroaki Matsunami, and Masatoshi Takeichi -- Flow cytometric analysis of whole organs and embryos / Jose Serna, Belen Pimentel, and Enrique J. de la Rosa -- Detection of multiple gene products simulaneously by in situ hybridization and immunohistochemistry in whole mounts of avian embryos / Claudio D. Stern -- Cloning of genes from single neurons / Catherine Dulac -- Methods for detecting and quantifying apoptosis / Nicola J. McCarthy and Gerard I. Evan -- Methods in drosophila cell cycle biology / Fabian Feiguin, Salud Llamazares, and Cayetano Gonzalez -- Single central nervous system neurons in culture / Juan Lerma, Miquel Morales, and Maria de los Angeles Vicente -- Patch-clamp recordings from drosophila presynaptic terminals / Manuel Martinez-Padron and Alberto Ferrus. 
520 |a This volume incorporates practical methods at the level of molecular, cellular, and whole organism biology in vertebrate and invertebrate models. It presents straightforward protocols written step-by-step for state-of-the-art techniques with the emphasis on single-cell resolution procedures. Key Features * Provides straightforward, current protocols and critical appraisals * Includes diverse analysis of cellular and molecular techniques * Presents everything from whole-organ cultures to electrophysiological approaches * Details a variety of methods for interfering with gene function in various species * Offers multiple illusions of in situ hybridization, immunostaining, and apoptosis. 
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700 1 |a De Pablo, Flora,  |e editor. 
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700 1 |a Stern, C. D.  |q (Claudio D.),  |e editor. 
776 0 8 |i Print version:  |t Current topics in developmental biology. Volume 36, Cellular and molecular procedures in developmental biology.  |d San Diego : Academic Press, �1998  |z 0121531368  |z 9780121531362  |w (OCoLC)37852228 
830 0 |a Current topics in developmental biology ;  |v v. 36. 
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