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Fluorescent proteins /
This new edition of Fluorescent Proteins presents current applications of autofluorescent proteins in cell and molecular biology authored by researchers from many of the key laboratories in the field. Starting from a current review of the broad palette of fluorescent proteins available, several chap...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam ; London :
Elsevier/Academic Press,
�2008.
|
| Edición: | 2nd ed. |
| Colección: | Methods in cell biology ;
v. 85. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
Tabla de Contenidos:
- Front Cover; Methods in Cell Biology; Copyright Page; Contents; Contributors; Preface; Chapter 1: Autofluorescent Proteins; A. Structure; B. Stability, Folding, and Multimerization; C. Spectra and Photophysical Dynamics; A. Multiple Labeling; B. Dynamic Imaging; C. Protein-Protein Interactions; References; Chapter 2: Functional Fusion Proteins by Random Transposon-Based GFP Insertion; B. The Transposition Reaction; C. Transformation Requirements and Troubleshooting; D.E.coli Colony Selection and Growth in a 96-Well Format; E. Backing Up the Experiment: Making 10% Glycerol Stocks.
- F. 96-Well Mini-Preparation Purification of Plasmid DNAG. Preparation of HEK 293 Cells; H. Transient Transfection of HEK 293 Cells in a 96-Well Format; I. Screening Live HEK 293 Cells for GEP Fluorescence; J. Removing the Selection Cassette with Restriction Digestion and Re-Ligation; IV. Materials; A. PCR Amplification of the Transposon; B. The Transposition Reaction; C. Transformation Requirements and Troubleshooting; D.E.coli Colony Selection and Growth in a 96-Well Format; E. Backing Up the Experiment: Making 10% Glycerol Stocks; F. 96-Well-Mini-Preparation Purification of Plasmid DNA.
- G. Preparation of HEK 293 CellsH. Transient Transfection of HEK 293 Cells in a 96-Well Format; I. Screening Live HEK 293 Cells for GEP Fluorescence; J. Removing the Selection Cassette with Restriction Digestion and Re-Ligation; V. Discussion; Acknowledgments; References; Chapter 3: Fluorescent Proteins for Photoactivation Experiments; A. Photoactivatable Fluorescent Proteins: Aequorea victoria GFP; B. Photoactivatable Fluorescent Proteins: DsRed Fluorescent Protein; C. Photoactivatable Fluorescent Proteins: Green-to-Red Photoconversions.
- D. Photoactivatable Fluorescent Proteins: Cyan-to-Green PhotoconversionE. Photoactivatable Fluorescent Proteins: Reversible; A. Protein Dynamics; B. Fluorescence Pulse-Labeling.; C. Photoquenching Fluorescence Resonance Energy Transfer; D. Photoactivated Localization Microscopy; References; Chapter 4: Design and Optimization of Genetically Encoded Fluorescent Biosensors: GTPase Biosensors; B. Expression of the Biosensor in Cells; A. DNA Sequence for the pTriEX-4-Biosensor Construct; VIII. Appendix II; A. Media Formulation for Ham's F-12K Phenol Red-Free; References.
- Chapter 5: Fast 4D MicroscopyA. Imaging Modes and Combination with Other Functionalities into a Multifunctional System; B. Keeping the Sampled Volume Immobile and Test for It; C. The Impact of Optical Blur, Noise, Aberrations, and Calibration Defects; D. Setting Up a Rapid 4D Acquisition; IV. Conclusions; Acknowledgments; References; Chapter 6: Single-Molecule Imaging of Fluorescent Proteins; References; Chapter 7: Counting Kinetochore Protein Numbers in Budding Yeast Using Genetically Encoded Fluorescent Proteins; III. Sample Preparation; IV. Microscope and Image Acquisition System.