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Bioengineering and molecular biology of plant pathways /
The increased knowledge about the structure of genomes in a number of species, about the complexity of transcriptomes, and the rapid growth in knowledge about mutant phenotypes have set off the large scale use of transgenes to answer basic biological questions, and to generate new crops and novel pr...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam ; Boston :
Pergamon,
2008.
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| Colección: | Advances in plant biochemistry and molecular biology ;
v. 1. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo Texto completo |
Tabla de Contenidos:
- Front Cover; Bioengineering and Molecular Biology of Plant Pathways; Copyright Page; Dedication Page; Contents; Contributors; Introduction to the Series and Acknowledgements; Preface to Volume 1; Prologue; Chapter 1: Metabolic Organization in Plants: A Challenge for the Metabolic Engineer; 1. Introduction; 2. Plant Metabolic Networks and Their Organization; 3. Tools for Analyzing Network Structure and Performance; 3.1. Constraints-based network analysis; 3.2. Metabolic flux analysis; 3.3. Kinetic modeling; 3.4. Metabolic control analysis; 4. Integration of Plant Metabolism
- 4.1. Relationship between enzyme properties and network fluxes4.2. Limitations on metabolic compensation within a network; 4.3. Impact of physiological conditions on network performance; 4.4. Network adjustments through alternative pathways; 4.5. Propagation of metabolic perturbations through networks; 4.6. Enzyme-specific responses within networks; 4.7. Impact of metabolic change on network structure; 5. Summary; Acknowledgements; References; Chapter 2: Enzyme Engineering; 1. Introduction; 2. Theoretical Considerations; 2.1. Enzyme architecture is conserved
- 2.2. Genomic analysis suggests most enzymes evolve from preexisting enzymes2.3. Evolution of a new enzymatic activity in nature; 2.4. The natural evolution process initially produces poor enzymes; 2.5. Sequence space and fitness landscapes; 3. Practical Considerations for Engineering Enzymes; 3.1. Identifying appropriate starting enzyme(s); 3.2. Ways of introducing variability into genes; 3.3. Choice of expression system; 3.4. Identifying improved variants; 3.5. Recombination and/or introduction of subsequent mutations; 3.6. Structure-based rational design
- 4. Opportunities for Plant Improvement Through Engineered Enzymes and Proteins4.1. Challenges for engineering plant enzymes and pathways; 5. Summary; Acknowledgements; References; Chapter 3: Genetic Engineering of Amino Acid Metabolism in Plants; 1. Introduction; 2. Glutamine, Glutamate, Aspartate, and Asparagine are Central Regulators of Nitrogen Assimilation, Metabolism, and Transport; 2.1. GS: A highly regulated, multifunctional gene family; 2.2. Role of the ferredoxin- and NADH-dependent GOGAT isozymes in plant glutamate biosynthesis
- 2.3. Glutamate dehydrogenase: An enzyme with controversial functions in plants2.4. The network of amide amino acids metabolism is regulated in concert by developmental, physiological, environmental, metabolic, and stress-derived signals; 3. The Aspartate Family Pathway that is Responsible for Synthesis of the Essential Amino Acids Lysine, Threonine, Methionine, and Isoleucine; 3.1. The aspartate family pathway is regulated by several feedback inhibition loops; 3.2. Metabolic fluxes of the aspartate family pathway are regulated by developmental, physiological, and environmental signals