Methods in Cell-Matrix Adhesion /
Critically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made a...
Clasificación: | Libro Electrónico |
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Otros Autores: | |
Formato: | Electrónico eBook |
Idioma: | Inglés |
Publicado: |
San Diego :
Academic Press,
2002.
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Colección: | Methods in cell biology ;
v. 69. |
Temas: | |
Acceso en línea: | Texto completo Texto completo Texto completo |
Tabla de Contenidos:
- pt. 1. Preface and perspectives on cell-matrix adhesion
- Cell-matrix interactions: the view from the outside / Paul Bornstein
- Matrix and meaning / Martin A. Schwartz
- Cell-matrix adhesion research and the development of biotherapeutics / Roy R. Lobb
- pt. 2. Matrix methodologies
- Detection and purification of instructive extracellular matrix components with monoclonal antibody technologies / Susana G. Gil
- Isolation and purification of proteoglycans / John M. Whitelock
- Expression of recombinant matrix components using baculoviruses / Deane F. Mosher
- Heparan sulfate
- growth factor interactions / Alan C. Rapraeger
- Analysis of basement membrane self-assembly and cellular interactions with native and recombinant glycoproteins / Peter D. Yurchenco
- Preparation and analysis of synthetic multicomponent extracellular matrix / Kim S. Midwood
- Analysis of matrix dynamics by atomic force microscopy / Helen G. Hansma
- Analysis of matrix degradation / H.G. Munshi
- pt. 3. Adhesion receptor methodologies
- Expression cloning strategies for the identification of adhesion molecules / Joe W. Ramos
- Purification of integrins and characterization of integrin-associated proteins / Johannes A. Eble
- Methods for analysis of the integrin ligand binding event / Jeffrey W. Smith
- Intracellular coupling of adhesion receptors: molecular proximity measurements / Maddy Parsons
- pt. 4. Functional applications of cell-matrix adhesion in molecular cell biology
- Functional analysis of cell adhesion: quantiation of cell-matrix attachment / Steven K. Akiyama
- Measurements of glycosaminoglycan-based cell interactions / J. Kevin Langford
- Applications of adhesion molecule gene knockout cell lines / Jordan A. Kreidberg
- Flexible polyacrylamide substrata for the analysis of mechanical interactions at cell-substratum adhesions / Karen A. Beningo
- Cell migration in slice cultures / Donna J. Webb
- Application of cell adhesion to study signaling networks / Cindy K. Miranti
- Use of micropatterned adhesive surfaces for control of cell behavior / Philip LeDuc
- Adenoviral-mediated gene transfer in two-dimensional and three-dimensional cultures of mammary epithelial cells / Harriet Watkin.