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Chromatin and chromatin remodeling enzymes. Part A /
DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the Chromatin remodellng enzymes play an important role in gene transcription. *Histone Bioinformatics *Biochemistry of histones, nucleosomes and chromatin *Molecular cytology of chromatin functions.
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam ; Boston :
Elsevier Academic Press,
�2004.
|
| Colección: | Methods in enzymology ;
v. 375. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
Tabla de Contenidos:
- 1. Mining core histone sequences from public protein databases
- 2. Reconstitution of nucleosome core particles from recombinant histones and DNA
- 3. Preparation and crystallization of nucleosome core particle
- 4. Creating designer histones by native chemical ligation
- 5. Two-dimensional gel analysis of histones and other H2AX-related methods
- 6. Assembly of yeast chromatin using ISWI complexes
- 7. Reconstitution of yeast chromatin using yNap1p
- 8. DNA synthesis-dependent and -independent chromatin assembly pathways in Xenopus egg extracts
- 9. Preparation of defined mononucleosomes, dinucleosomes, and nucleosome arrays in vitro and analysis of transcription factor binding
- 10. Purification of native, defined chromatin segments
- 11. Purification of defined chromosomal domains
- 12. Probing core histone tail-DNA interactions in a model dinucleosome system
- 13. Site-directed histone-DNA contact mapping for analysis of nucleosome dynamics
- 14. Site-specific attachment of reporter compounds to recombinant histones
- 15. Histone MacroH2A purification and nucleosome reconstitution
- 16. Analysis of histone variant H2A.Z localization and expression during early development
- 17. Histone variant CENP-A purification, nucleosome reconstitution
- 18. Immunological analysis and purification of centromere complex
- 19. Purification and analyses of histone H1 variants and H1 posttranslational modifications
- 20. HMGA proteins: isolation, biochemical modifications, and nucleosome interactions
- 21. Preparation and functional analysis of HMGN proteins
- 22. Methods for visualizing chromatin dynamics in living yeast
- 23. Direct visualization of transcription factor-induced chromatin remodeling and cofactor recruitment in vivo
- 24. Measuring histone and polymerase dynamics in living cells
- 25. Measurement of dynamic protein binding to chromatin in vivo, using photobleaching microscopy
- 26. Quantification of protein-protein and protein-DNA interactions in vivo, using fluorescence recovery after photobleaching
- 27. Fluorescence recovery after photobleaching (FRAP) methods for visualizing protein dynamics in living mammalian cell nuclei
- 28. Correlative light and electron spectroscopic imaging of chromatin in situ
- 29. RNA fluorscence in situ hybridization tagging and recovery of associated proteins to analyze in vivo chromatin interactions
- 30. 3C technology: analyzing the spatial organization of genomic loci in vivo / Erik Splinter, Frank Grosveld, and Wouter de Laat.