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G protein pathways. Part C, Effector mechanisms /

This third volume in the trio covering G proteins, features integrated approaches to studying G proteins. Methods pertaining to signaling mechanisms are presented, including theoretical and modeling approaches, biochemistry and molecular biology, and cell biology and physiology. The techniques for s...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Iyengar, Ravi, Hildebrandt, John D.
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego : Academic Press, �2002.
Colección:Methods in enzymology ; v. 345.
Temas:
Acceso en línea:Texto completo
Texto completo

MARC

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245 0 0 |a G protein pathways.  |n Part C,  |p Effector mechanisms /  |c edited by Ravi Iyengar, John D. Hildebrandt. 
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490 1 |a Methods in enzymology ;  |v v. 345 
504 |a Includes bibliographical references and indexes. 
588 0 |a Print version record. 
520 |a This third volume in the trio covering G proteins, features integrated approaches to studying G proteins. Methods pertaining to signaling mechanisms are presented, including theoretical and modeling approaches, biochemistry and molecular biology, and cell biology and physiology. The techniques for studying the structure and function of G proteins are important not only to those with specific research interests in them, but also endocrinologists and pharmacologists conducting research on signaling mechanisms that are increasingly understood to interact with G proteins. 
505 0 |a Front Cover; G Protein Pathways; Copyright Page; Table of Contents; Contributors to Volume 345; Preface; Volume in Series; Section I: Modeling Intracellular Signaling Pathways; Chapter 1. Use of Kinetikit and GENESIS for Modeling Signaling Pathways; Section II: Phosphodiesterases; Chapter 2. Assays of G Protein/cGMP-Phosphodiesterase Interactions; Chapter 3. Assaying G Protein-Phosphodiesterase Interactions in Sensory Systems; Section III: Calcium and Potassium Channels; Chapter 4. Studies of Endogenous G-Protein-Mediated Pathways in Neurons by Whole-Cell Electrophysiology 
505 8 |a Chapter 5. Biochemical Approaches to Study Interaction of Calcium Channels with RGS12 in Primary Neuronal CulturesChapter 6. Assaying Phosphatidylinositol Bisphosphate Regulation of Potassium Channels; Section IV: Adenylyl Cyclases; Chapter 7. Purification of Soluble Adenylyl Cyclase; Chapter 8. Calcium-Sensitive Adenylyl Cyclase/Aequorin Chimeras as Sensitive Probes for Discrete Modes of Elevation of Cytosolic Calcium; Chapter 9. Kinetic Analysis of the Action of P-Site Analogs 
505 8 |a Chapter 17. Construction of Soluble Adenylyl Cyclase from Human Membrane-Bound Type 7 Adenylyl CyclaseChapter 18. Genetic Selection of Regulatory Mutants of Mammalian Adenylyl Cyclases; Section V: Phospholipases and Lipid-Derived Products; Chapter 19. Expression and Characterization of Rat Brain Phospholipase D; Chapter 20. G-Protein-Coupled Receptor Regulation of Phospholipase D; Chapter 21. Analysis and Quantitation of Ceramide; Chapter 22. Assays for Phospholipase D Reaction Products 
505 8 |a Chapter 23. Determination of Strength and Specificity of Membrane-Bound G Protein-Phospholipase C Association Using Fluorescence SpectroscopyChapter 24. Assays and Characterization of Mammalian Phosphatidylinositol 4,5-Bisphosphate-Sensitive Phospholipase D; Chapter 25. Characterization and Purification of Phosphatidylinositol Trisphosphate 5-Phosphatase from Rat Brain Tissues; Section VI: Small GTP-Binding Proteins; Chapter 26. Assay of Cdc42, Rac, and Rho GTPase Activation by Affinity Methods; Chapter 27. Assays of ADP-Ribosylation Factor Function 
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650 0 |a Enzymes. 
650 0 |a Ion channels. 
650 0 |a Cellular signal transduction. 
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700 1 |a Iyengar, Ravi. 
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776 0 8 |i Print version:  |t G protein pathways. Part C, Effector mechanisms.  |d San Diego : Academic Press, �2002  |z 012182246X  |z 9780121822460  |w (OCoLC)48116848 
830 0 |a Methods in enzymology ;  |v v. 345. 
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