Cargando…

Bioluminescence and chemiluminescence. Part C /

Since the publication of Bioluminescence and Chemiluminescence, Part B, genes have been cloned that encode luciferases from an array of bioluminescent organisms, novel applications of these genes have been developed, and much has been learned of the fundamental chemistry, biochemistry, structural bi...

Descripción completa

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Ziegler, Miriam M., Baldwin, Thomas O.
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego : Academic Press, �2000.
Colección:Methods in enzymology ; v. 305.
Temas:
Acceso en línea:Texto completo
Texto completo

MARC

LEADER 00000cam a2200000 a 4500
001 SCIDIR_ocn164583772
003 OCoLC
005 20231117015041.0
006 m o d
007 cr cnu---unuuu
008 070815s2000 caua ob 001 0 eng d
040 |a N$T  |b eng  |e pn  |c N$T  |d YDXCP  |d OCLCQ  |d UBY  |d IDEBK  |d E7B  |d OCLCQ  |d COF  |d OCLCQ  |d OPELS  |d OCLCQ  |d OPELS  |d OCLCF  |d KUK  |d OCLCQ  |d BUF  |d OCLCQ  |d KIJ  |d BRX  |d ANS  |d WYU  |d ESU  |d YOU  |d SOI  |d OCLCO  |d LEAUB  |d OCLCQ  |d DCT  |d RDF  |d OCLCA  |d OCLCQ  |d OCLCO  |d S2H  |d OCLCO  |d OCLCQ  |d OCLCO  |d INARC  |d OCLCQ  |d OCLCO 
019 |a 505144742  |a 648270052  |a 742283805  |a 814451442  |a 823106952  |a 823827189  |a 823896656  |a 824088740  |a 824135124  |a 906429816  |a 1027332757 
020 |a 9780080496641  |q (electronic bk.) 
020 |a 0080496644  |q (electronic bk.) 
020 |a 0121822060  |q (electronic bk.) 
020 |a 9780121822064  |q (electronic bk.) 
020 |a 1281010936 
020 |a 9781281010933 
035 |a (OCoLC)164583772  |z (OCoLC)505144742  |z (OCoLC)648270052  |z (OCoLC)742283805  |z (OCoLC)814451442  |z (OCoLC)823106952  |z (OCoLC)823827189  |z (OCoLC)823896656  |z (OCoLC)824088740  |z (OCoLC)824135124  |z (OCoLC)906429816  |z (OCoLC)1027332757 
050 4 |a QH641  |b .B56 2000eb 
060 4 |a QU135  |b MET 
072 7 |a SCI  |x 007000  |2 bisacsh 
072 7 |a PSBZ  |2 bicssc 
082 0 4 |a 572.4358  |2 22 
245 0 0 |a Bioluminescence and chemiluminescence.  |n Part C /  |c edited by Miriam M. Ziegler, Thomas O. Baldwin. 
260 |a San Diego :  |b Academic Press,  |c �2000. 
300 |a 1 online resource (xxxv, 732 pages) :  |b illustrations (some color) 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
347 |a data file 
490 1 |a Methods in enzymology ;  |v v. 305 
504 |a Includes bibliographical references and index. 
588 0 |a Print version record. 
520 |a Since the publication of Bioluminescence and Chemiluminescence, Part B, genes have been cloned that encode luciferases from an array of bioluminescent organisms, novel applications of these genes have been developed, and much has been learned of the fundamental chemistry, biochemistry, structural biology, and biophysics of these intriguing enzymes. New strategies for application of chemiluminescence technology have been developed and refined, promising to further reduce the need to use radioisotopes in basic research and clinical laboratory settings. Methods for detection of low levels of light continue to push the limits of detection, allowing ready monitoring in real time of intricate subcellular processes within living cells. This book affords a glimpse of the state of the art of a rapidly advancing field, and presents to users of these methods a detailed reference of current activities in the field. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. Key Features @bul* Outlines the use of luminescent reporter technologies to monitor gene expression and protein trafficking * Describes the luminescence-based clinical assay technologies * Details the basic biochemistry, biophysics, and chemistry of light-emitting reactions that are critical for applications * Includes explanations of the instrumentation used for detection and quantification of low level light * Shows the new applications of luminescence-based technologies that result largely from broad advances in recombinant DNA technologies and nonscale methods. 
505 0 |a Front Cover; Bioluminescence and Chemiluminescence; Copyright Page; Table of Contents; Contributors to Volume 305; Preface; Volumes in Series; Section I: Chemiluminescence and Bioluminescence: Overviews; Chapter 1. Chemical Generation of Excited States: The Basis of Chemiluminescence and Bioluminescence; Chapter 2. Some Brief Notes on Nomenclature and Units and Standards Used in Bioluminescence and Chemiluminescence; Section II: Instrumentation; Chapter 3. Physics of Low Light Detectors; Chapter 4. Luminometer Design and Low Light Detection 
505 8 |a Chapter 5. Absolute Calibration of Luminometers with Low-Level Light StandardsChapter 6. Commercially Available Luminometers and Low-Level Light Imaging Devices; Chapter 7. Automated Recordings of Bioluminescence with Special Reference to the Analysis of Circadian Rhythms; Chapter 8. Chemiluminescence Imaging Systems for the Analysis of Macrosamples: Microtiter Format, Blot Membrane, and Whole Organs; Section III: Luciferases, Luminescence Accessory Proteins, and Substrates; Chapter 9. Overexpression of Bacterial Luciferase and Purification from Recombinant Sources 
505 8 |a Chapter 10. Purification of Luciferase Subunits from Recombinant SourcesChapter 11. A Rapid Chromatographic Method to Separate the Subunits of Bacterial Luciferase in Urea Containing Buffer; Chapter 12. Purification and Ligand Exchange Protocols for Antenna Proteins from Bioluminescent Bacteria; Chapter 13. Purification of Firefly Luciferase from Recombinant Sources; Chapter 14. Chemical Synthesis of Firefly Luciferin Analogs and Inhibitors; Chapter 15. Structural Basis for Understanding Spectral Variations in Green Fluorescent Protein 
505 8 |a Chapter 16. Large-Scale Purification of Recombinant GFP from Escherichia coliChapter 17. Recombinant Obelin: Cloning and Expression of cDNA, Purification, and Characterization as a Calcium Indicator; Chapter 18. Gonyaulax Luciferase: Gene Structure, Protein Expression, and Purification from Recombinant Sources; Chapter 19. Dinoflagellate Luciferin-Binding Protein; Section IV: Bacterial Autoinduction System and Its Applications; Chapter 20. Assay of Autoinducer Activity with Luminescent Escherichia coli Sensor Strains Harboring a Modified Vibrio fischeri lux Regulon 
505 8 |a Chapter 21. Detection, Purification, and Structural Elucidation of the Acylhomoserine Lactone Inducer of Vibrio fischeri Luminescence and Other Related MoleculesChapter 22. Chemical Synthesis of Bacterial Autoinducers and Analogs; Chapter 23. Overexpression of Foreign Proteins Using the Vibrio fischeri lux Control System; Section V: Luminescence-Based Assays in Vitro; Chapter 24. Application of Bioluminescence and Chemiluminescence in the Biomedical Sciences; Chapter 25. Use of Firefly Luciferase in ATP-Related Assays of Biomass, Enzymes, and Metabolites 
650 0 |a Bioluminescence. 
650 0 |a Chemiluminescence. 
650 1 2 |a Luciferases  |x metabolism  |0 (DNLM)D008156Q000378 
650 2 2 |a Luminescent Measurements  |0 (DNLM)D008163 
650 6 |a Bioluminescence.  |0 (CaQQLa)201-0020319 
650 6 |a Chimiluminescence.  |0 (CaQQLa)201-0034748 
650 7 |a chemiluminescence.  |2 aat  |0 (CStmoGRI)aat300191614 
650 7 |a SCIENCE  |x Life Sciences  |x Biochemistry.  |2 bisacsh 
650 7 |a Bioluminescence  |2 fast  |0 (OCoLC)fst00832517 
650 7 |a Chemiluminescence  |2 fast  |0 (OCoLC)fst00853328 
700 1 |a Ziegler, Miriam M. 
700 1 |a Baldwin, Thomas O. 
776 0 8 |i Print version:  |t Bioluminescence and chemiluminescence. Part C.  |d San Diego : Academic Press, �2000  |z 0121822060  |z 9780121822064  |w (OCoLC)44438820 
830 0 |a Methods in enzymology ;  |v v. 305. 
856 4 0 |u https://sciencedirect.uam.elogim.com/science/book/9780121822064  |z Texto completo 
856 4 0 |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/305  |z Texto completo