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Molecular imaging : FRET microscopy and spectroscopy /
The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and t...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor Corporativo: | |
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Oxford ; New York :
Published for the American Physiological Society by Oxford University Press,
2005.
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| Colección: | Methods in physiology series.
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| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 245 | 0 | 0 | |a Molecular imaging : |b FRET microscopy and spectroscopy / |c edited by Ammasi Periasamy, Richard N. Day. |
| 260 | |a Oxford ; |a New York : |b Published for the American Physiological Society by Oxford University Press, |c 2005. | ||
| 300 | |a 1 online resource (xv, 312 pages) : |b illustrations (some color) | ||
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| 490 | 1 | |a The American Physiological Society methods in physiology series | |
| 520 | |a The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuable information about the preparation of biological samples used for FRET image analysis is also provided. The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRET experiments and the selection of the most suitable experimental approaches to address specific biological questions. Up-to-date, consistently organized, and well-illustrated, this unique book will be welcomed by all researchers who wish to use FRET microscopy and spectroscopy techniques. | ||
| 505 | 0 | |a Proteins and the Flow of Information in Cellular Function; Basics of Fluorescence and FRET; An Introducrion to Filters and Mirrors for FRET; FRET Imaging in the Wide-field Microscope; Confocal FRET Microscopy -- Study of Clustered Distribution of Receptor-ligand Complexes in Endocytic Membranes; Multiphoton FRET Microscopy for Protein Localization in Tissue; Single-Molecule FRET; FRET Measurements using Multispectral Imaging; Real-time Fluorescence Lifetime Imaging and FRET using Fast Gated Image Intensifiers; Streak FLIM: A Novel Technology for Quantitative FRET Imaging; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization;Bioluminescence RET (BRET): Techniques and Potential; Quantifying Molecular Interactions with Fluorescence Correlation Spectrosocpy; Mapping Molecular Interactions and Transport in Cell Membranes by Image Correlation Spectroscopy; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization. | |
| 504 | |a Includes bibliographical references and index. | ||
| 588 | 0 | |a Print version record. | |
| 506 | |3 Use copy |f Restrictions unspecified |2 star |5 MiAaHDL | ||
| 533 | |a Electronic reproduction. |b [Place of publication not identified] : |c HathiTrust Digital Library, |d 2010. |5 MiAaHDL | ||
| 538 | |a Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. |u http://purl.oclc.org/DLF/benchrepro0212 |5 MiAaHDL | ||
| 583 | 1 | |a digitized |c 2010 |h HathiTrust Digital Library |l committed to preserve |2 pda |5 MiAaHDL | |
| 546 | |a English. | ||
| 650 | 0 | |a Fluorescence microscopy. | |
| 650 | 0 | |a Fluorescence spectroscopy. | |
| 650 | 2 | |a Microscopy, Fluorescence |0 (DNLM)D008856 | |
| 650 | 2 | |a Microscopy, Fluorescence |x methods |0 (DNLM)D008856Q000379 | |
| 650 | 2 | |a Spectrometry, Fluorescence |0 (DNLM)D013050 | |
| 650 | 2 | |a Spectrometry, Fluorescence |x methods |0 (DNLM)D013050Q000379 | |
| 650 | 2 | |a Fluorescence Resonance Energy Transfer |0 (DNLM)D031541 | |
| 650 | 6 | |a Microscopie de fluorescence. |0 (CaQQLa)201-0014904 | |
| 650 | 6 | |a Spectroscopie de fluorescence. |0 (CaQQLa)201-0066421 | |
| 650 | 7 | |a fluorescence microscopy. |2 aat |0 (CStmoGRI)aat300252489 | |
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| 653 | 0 | 0 | |a microscopie |
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| 653 | 0 | 0 | |a biological techniques |
| 653 | 0 | 0 | |a celbiologie |
| 653 | 0 | 0 | |a cellular biology |
| 653 | 0 | 0 | |a beeldvormende spectroscopie |
| 653 | 0 | 0 | |a imaging spectroscopy |
| 653 | 1 | 0 | |a Cellular Biology |
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| 653 | 2 | 0 | |a Biologische technieken |
| 700 | 1 | |a Periasamy, Ammasi. | |
| 700 | 1 | |a Day, Richard N. | |
| 710 | 2 | |a American Physiological Society (1887- ) | |
| 776 | 0 | 8 | |i Print version: |t Molecular imaging. |d Oxford ; New York : Published for the American Physiological Society by Oxford University Press, 2005 |z 0195177207 |z 9780195177206 |w (DLC) 2004061702 |w (OCoLC)57406048 |
| 830 | 0 | |a Methods in physiology series. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780195177206 |z Texto completo |