Human stem cell manual : a laboratory guide /

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Stem cells are self-replicating and undifferentiated, meaning their function is not yet cell, tissue, or organ-specific. Due to the unique nature of these cells, research into their biology and function holds great promise for therapeutic applications through replacement or repair of diseased and da...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Loring, Jeanne Frances, 1950-, Wesselschmidt, Robin L., Schwartz, Philip H.
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Amsterdam ; New York : Academic Press, 2007.
Temas:
Stem cells > Laboratory manuals.
Embryonic stem cells.
Biology > Research > Methodology.
Adult Stem Cells
Biomedical Research > methods
Cellules souches > Manuels de laboratoire.
Cellules souches embryonnaires.
Biologie > Recherche > M�ethodologie.
MEDICAL > Research.
Biology > Research > Methodology
Embryonic stem cells
Stem cells
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Foreword by James Battey
  • Foreword by Jeannie Fontana
  • Preface
  • Part I: Basic methods: Maintenance of stem cell cultures
  • 1. Human Embryonic Stem Cell Culture
  • 2. Feeder layers and feeder-free culture
  • 3. Mouse Embryonic Fibroblast Feeder Cells
  • 4. Cryopreservation of hESC
  • Part II: Basic methods: Characterization of stem cells
  • 5. Classical Cytogenetics: Karyotyping
  • 6. Spectral Karyotyping and Fluorescent in situ Hybridization
  • 7. Using SNP Genotyping to Identify Cell Lines, Determine Genomic Abnormalities and Monitor DNA Methylation Patterns
  • 8. FACS/Cell sorting
  • 9. Fluorescence Immunocytochemical Analysis of Stem Cells
  • 10. Characterization of Stem Cells Using RT-PCR
  • 11. Gene Expression Profiling of Stem Cells by Microarray
  • 12. Teratomas produced from Human Embryonic Stem Cells Xenografted into Immunodeficient Mice
  • 13. Generation of HESC-Derived Teratomas
  • Part III: Differentiation of stem cells
  • 14. Embryoid Body Formation and Neuroepithelia Differentiation
  • 15. Motor Neuron and Dopamine Neuron Differentiation
  • 16. Oligodendrocyte Differentiation from hESC
  • 17. Cardiac
  • 18. Hematopoietic
  • Part IV: Genetic manipulation of stem cells
  • 19. Genetic Manipulation of hESC: Lentivirus Vectors
  • 20. Methods developed for mouse ES cells
  • Part V: Advanced methods
  • 21. Derivation of human ES cells
  • 22. Neural Stem Cell Culture
  • 23. Transplantation of stem cells
  • 24. In vitro fertilization
  • 25. Functional characterization-neural cells
  • Part VI: Practical issues in establishing a human ES cell laboratory
  • 26. Setting Up a Facility for hESC Research
  • Part VII: Essays on stem cell biology
  • 27. Intellectual Property: Owning the Stem Cell
  • 28. Ethical Concerns for Stem Cell Research
  • 29. Guidelines for hESC Research Oversight (ESCRO) Committees.
  • Basic methods in stem cell culture
  • Characterization of stem cells
  • Differentiation of human embryonic cells
  • Genetic manipulation of stem cells
  • Advanced methods
  • Setting up a laboratory
  • Stem cells and society.

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