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Viral gene techniques /

Viral Gene Techniques is a practical laboratory guide to current techniques of molecular biology and genetics. The volume is concerned with methods for the analysis of viral genes and chromosomes: DNA viruses and RNA viruses including HIV are discussed. * Methods presented for ease of use and reasdy...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Adolph, Kenneth W., 1944-
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego : Academic Press, �1995.
Colección:Methods in molecular genetics ; v. 7.
Temas:
Acceso en línea:Texto completo
Texto completo
Texto completo

MARC

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245 0 0 |a Viral gene techniques /  |c edited by Kenneth W. Adolph. 
260 |a San Diego :  |b Academic Press,  |c �1995. 
300 |a 1 online resource (xvii, 416 pages) :  |b illustrations 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in molecular genetics,  |x 1067-2389 ;  |v v. 7 
520 |a Viral Gene Techniques is a practical laboratory guide to current techniques of molecular biology and genetics. The volume is concerned with methods for the analysis of viral genes and chromosomes: DNA viruses and RNA viruses including HIV are discussed. * Methods presented for ease of use and reasdy adaptation to new systems * Detailed experimental protocols included for: * Viral vectors - construction and use of DNA virus vectors (adenovirus, adeno-associated virus, vaccinia virus, Epstein - Barr virus) * DNA viruses - virus/host interactions, viral chromosomes, transcription regulation (viruses discussed include herpes simplex, hepatitis B, SV40, JC, Epstein-Barr, adenovirus) * Human Immunodeficiency Virus / retroviruses -quantitation of HIV-1 virus stock and RNA, retrovirus reverse transcription / integration, retrovirus-mediated cell fusion, use as cell lineage markers * RNA viruses - RNA virus assembly, analysis of RNA genomes, assays for RNA-binding proteins (viruses discussed include poliovirus, influenza virus, hepatitis delta virus). 
505 0 |a Viral Vectors: Adeno-Associated Virus: Integrating Vectors for Human Gene Therapy. Techniques for Human Adenovirus Vector Construction and Characterization. Construction and Isolation of Recombinant Adenoviruses with Gene Replacements. Construction and Use of Recombinant Vaccinia Virus Vectors. Epstein-Barr Viral Plasmid Vectors and Their Amplifiable Derivatives. DNA Viruses: Methods to Study Epstein-Barr Virus and P53 Status in Human Cells. The SV40 Minichromosome. Site-Specific Integration of Multigenic Shuttle Plasmids Into the Herpes Simplex Virus Type 1 (HSV. 1). Genome Using a Cell-Free Cre-Lax Recombination System. Strategies Transcription Regulation of Neurotropic Viruses in CNS: Lesson from JC Virus. Analysis of the Nuclear DNA-Binding Activity in Cells Overexpressing Hepatitus B Viral X and 3'-Truncated S Transactivators. Investigations on Virus-Host Interations: An Abortive Systems. Human Immunodeficiency Virus/Retroviruses: Quantitation of Virus Stocks Produced from Cloned Human Immunodeficiency Virus DNA. Quantitation of HIV-1 RNA in Plasma or Serum Samples. Detection of Cell Fusion Mediated By the Envelopes of Human Retroviruses By Transactivation of a Reporter Gene. Assembly of Recombinant Retroviral Gag Precursors Into Pseudovirions in the Baculovirus-Insect Cell Expression System. Retrovirus Reverse Transcription and Integration. In Vitro Binding and Transcription Assays Using the Human T-Cell Leukemia Type I Tax Protein. PCR-Based Techniques for Utilizing Retroviruses as Cell Lineage Markers. RNA Viruses: New Methods to Study Poliovirus Assembly and Encapsidation of Genomic RNA. Molecular Studies of Hepatitis Delta Virus Rnas. Systems to Express Recombinant RNA Molecules By the Influenza a Virus Polymerase in Vivo. Analysis of Membrane -Association in Vivo and Targeting of Viral Proteins in Polarized Epithelial Cells. Cloning of Viral Dsrna Genomes By Single Primer Amplification. Using the RNA-Capture Assay to Assess the RNA-Binding Activity of Viral Proteins. Protein RNA-Binding Activity Measured By Northwestern Blot Analysis: The Interferon-Inducible, RNA-Dependent Protein Kinase PKR. Index. 
504 |a Includes bibliographical references and index. 
588 0 |a Print version record. 
506 |3 Use copy  |f Restrictions unspecified  |2 star  |5 MiAaHDL 
533 |a Electronic reproduction.  |b [Place of publication not identified] :  |c HathiTrust Digital Library,  |d 2010.  |5 MiAaHDL 
538 |a Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.  |u http://purl.oclc.org/DLF/benchrepro0212  |5 MiAaHDL 
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546 |a English. 
650 0 |a Viral genetics. 
650 0 |a Genetics  |x Technique. 
650 2 |a Genes, Viral  |x genetics  |0 (DNLM)D005814Q000235 
650 2 |a Genetic Techniques  |0 (DNLM)D005821 
650 2 |a Adenoviridae  |x genetics  |0 (DNLM)D000256Q000235 
650 2 |a DNA Viruses  |x genetics  |0 (DNLM)D004267Q000235 
650 2 |a RNA Viruses  |x genetics  |0 (DNLM)D012328Q000235 
650 2 |a Retroviridae  |x genetics  |0 (DNLM)D012190Q000235 
650 6 |a G�en�etique virale.  |0 (CaQQLa)201-0008932 
650 6 |a G�en�etique  |x Technique.  |0 (CaQQLa)201-0068339 
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653 0 |a Viruses  |a Genetics 
655 4 |a Electronic books. 
700 1 |a Adolph, Kenneth W.,  |d 1944- 
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