Molecular and diagnostic procedures in mycoplasmology /

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This book and its companion, Volume II, concentrate on new procedures--especially those based on the new molecular methodology--developed within the past decade. This volume outlines the approaches, techniques, and procedures applied to cell and molecular biology studies of mycoplasmas. Volume II de...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Razin, Shmuel, Tully, Joseph G.
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego : Academic Press, �1995-�1996.
Temas:
Mycoplasma diseases > Diagnosis.
Mycoplasma diseases > Molecular aspects.
Mycoplasma > physiology
Mycoplasma > pathogenicity
Mycoplasma Infections > diagnosis
Molecular Biology > methods
Mycoplasmoses > Aspect mol�eculaire.
Mycoplasma diseases > Diagnosis
Mycoplasma diseases > Molecular aspects
Electronic books.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • S. Razin, Molecular Properties of Mollicutes: A Synopsis.
  • Cultivation and Morphology:
  • J.G. Tully, Introductory Remarks.
  • J.G. Tully, Culture Medium Formulation for Primary Isolation and Maintenance of Mollicutes.
  • K.J. Hackett and R.F. Whitcomb, Cultivation of Spiroplasmas in Undefined and Defined Media.
  • K.J. Hackett and D.E. Lynn, Insect Cell Culture Approaches in Cultivating Spiroplasmas.
  • J.A. Robertson and G.W. Stemke, Measurement of Mollicutes Growth by ATP-Dependent Luminometry.
  • D. Taylor-Robinson, Intracellular Location of Mycoplasmas.
  • D.J. Wear and S.-C. Lo, Localization of Mycloplasmas in Tissues.
  • D.C. Krause and M.K. Stevens, Localization of Antigens on Mycoplasma Cell Surface and Tip Structures.
  • Genome Characterization and Genetics:
  • S. Razin, Introductory Remarks.
  • B.C. Kirkpatrick, N.A. Harrison, I.-M. Lee, H. Neimark, and B.B. Sears, Isolation of Mycoplasma-Like Organism DNA from Plant and InsectHosts.
  • H. Neimark and P. Carle, Mollicute Chromosome Size Determination and Characterization of Chromosomes from Uncultured Mollicutes.
  • T. Proft and R. Herrmann, Physical and Genetic Mapping.
  • K. Dybvig, Characterization of Virus Genomesand Extrachromosomal Elements.
  • J. Renaudin and J.-M. Bove, Plasmid and Viral Vectors for Gene Cloning and Expression in Sprioplasma citri.
  • K. Dybvig, G.E. Gasparich, and K.W. King, Artificial Transformation of Mollicutes via Polyethylene Glycol- and Electroporation-Mediated Methods.
  • A. Razin and P. Renbaum, DNA Methylation Analysis.
  • B. Bhugra and K. Dybvig, Identification and Characterization of Genome Rearrangements.
  • P. Renbaum and A. Razin, Expression of Mycoplasmal Genesin E. Coli.
  • Membrane Characterization:
  • S. Razin, Introductory Remarks.
  • A. Wieslander, S. Nystrom, and A. Dahlqvist, Posttranslational Modification of Membrane Proteins.
  • K.S. Wise, M.F. Kim, and R. Watson-McKown, Variant Membrane Proteins.
  • S. Rottem and M. Tarshis, Membrane Fusion.
  • U. Schummer and H.G. Schiefer, Mycoplasma Membrane Potentials.
  • S. Rottem, Ion Flow and Cell Volume.
  • Cell Metabolism:
  • S. Razin, Introductory Remarks.
  • J.D. Pollack, Methods for Testing Metabolic Activities in Mollicutes.
  • R.J. Miles, Rapid Microcalorimetric and Eletroanalytical Measurements of Metabolic Activities.
  • C.C. Dascher and J. Maniloff, Characterization of Heat Shock Proteins.
  • F.C. Minion and K.J. Jarvill-Taylor, Nucleolytic Activities of Mycoplasmas.
  • T. Watanabe and K.-I. Shibata, Proteolytic Activities.
  • S. Rottem and M. Salman, Phospholipase Activity in Mycoplasmas.
  • Taxonomy and Phylogeny:
  • S. Razin, Introductory Remarks.
  • J.G. Tully and R.F. Whitcomb, Minimal Standards for Description of New Species of Class Mollicutes.
  • W.G. Weisburg, Ribosomal RNA Sequencing and Construction of Mycoplasma Phylogenies.
  • S. Razin and D. Yogev, Restriction Endonuclease Analysis.
  • D. Yogev and S. Razin, Southern Blot Analysis and Ribotyping.
  • B. Schneider, E. Seemueller, C.D. Smart, and B.C. Kirkpatrick, Phylogenetic Classification of Plant Pathogenic Mycoplasma-like Organisms or Phytoplasmas.
  • J.G. Tully, Determination of Cholesterol and Polyoxyethylene Sorbitan Growth Requirements of Mollicutes.
  • Pathogenicity:
  • S. Razin, Introductory Remarks.
  • I. Kahane and E. Jacobs, Mycoplasma Adherence to Host Cells: Methods of Quantifying Adherence.
  • E. Jacobs, Mycoplasma Adherence to Host Cells: Epitope Mapping of Adhesins.
  • I. Kahane, Oxidative Damage Induced by Mycoplasmas.
  • R. Gallily, A. Avron, G. Jahns-Streubel, and P.F. Muhlradt, Activation of Macrophages and Monocytes by Mycoplasmas.
  • B.C. Cole and C.L. Atkin, Identification, Characterization, and Purification of Mycoplasmal Superantigens.
  • Y. Naot, Mycoplasmal B-Cell Mitogens.
  • P.M. Stuart and J.G. Woodward, Modulation of Expression of Major HistocompatibilityComplex Molecules by Mycoplasmas.
  • W.C. Lai and M. Bennett, Interaction of Mycoplasmas with Natural Killer Cells.
  • Subject Index.
  • v. 1. Molecular characterization
  • v. 2. Diagnostic procedures.

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