Cargando…
Techniques in protein chemistry VIII /
Praise for the Series: "The mainly sharp scientific focus of this set of snapshots is a credit to both the contricutors and the editorial team."--Biotechnology and Applied Biochemistry Techniques in Protein Chemistry VIII is the latest volume in this successful series. As a valuable bench-...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor Corporativo: | |
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego ; London :
Academic Press,
�1997.
|
| Colección: | Techniques in Protein Chemistry.
|
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
Tabla de Contenidos:
- Foreword.
- Preface.
- Acknowledgments.
- Primary Structural Analysis: Protein Sequencing Using Microreactors and Capillary Electrophoresis with Thermo-optical Absorbance Detection:
- Enhancement of Concentration Limits of Detection in Capillary Electrophoresis: Examples of On-Line Sample Preconcentration, Cleanup, and Microreactor Technology in Protein Characterization.
- Sequencing MHC Class I Peptides Using Membrane Preconcentration-Capillary Electrophoresis Tandem Mass Spectrometry (mPC-CE-MS/MS).
- Nano-electrospray Mass Spectrometry and Edman Sequencing of Peptides and Proteins Collected from Capillary Electrophoresis.
- Characterization of a Recombinant Hepatitis E Protein Vaccine Candidate by Mass Spectrometry and Sequencing Techniques.
- Comparison of the High Sensitivity and Standard Versions of Applied Biosystems Procise <V>TM</V> 494 N-Terminal Protein Sequencers Using Various Sequencing Supports.
- Evaluation of ABRF-96SEQ: A Sequence Assignment Exercise.
- Internal Protein Sequencing of SDS-PAGE-Separated Proteins: Optimization of an In Gel Digest Protocol.
- A Strategy to Obtain Internal Sequence Information from Blotted Proteins after Initial N-terminal Sequencing.
- Internal Protein Sequencing of SDS PAGE-Separated Proteins: A Collaborative ABRF Study.
- Physical and Chemical Analysis:
- Chromatographic Determination of Extinction Coefficients of Non-Glycosylated Proteins Using Refractive Index (RI) and UV Absorbance (UV) Detectors: Applications for Studying Protein Interactions by Size Exclusion Chromatography with Light-Scattering, UV, and RI Detectors.
- Single Alkaline Phosphatase Molecule Assay by Capillary Electrophoresis Laser-Induced Fluorescence Detection.
- A New Centrifugal Device Used in Sample Clean-up and Concentration of Peptides.
- Sample Preparation Using Synthetic Membrances for the Study of Biopolymers by Matrix Assisted Laser Desorption/Ionization Mass Spectrometry.
- Use of LC/MS Peptide Mapping for Characteriation of Isoforms in <V>15</V>N-Labeled Recombinant Human Leptin.
- Hyphenated HPLC Methodology for the Resolution and Elucidation of Peptides from Proteolytic Digests.
- Detecting and Identifying Active Compounds from a Combinatorial Library Using Iasys and Electrospray Mass Spectrometry.
- Amino Acid Analysis of Unusual and Complex Samples Based on 6-Aminoquinolyl-N-hydroxysuccinimidyl Carbamate Derivatization.
- Development of a Method for Analysis of Free Amino Acids from Physiological Samples Using a 420A ABI/PE Amino Acid Analyzer.
- Quantitation and Identification of Proteins by Amino Acid Analysis: ABRF-96AAA Collaborative Trial
- Chemical Modification:
- Nonaqueous Chemical Modification of Lyophilized Proteins
- Reaction of HIV-1 NC p7 Zinc Fingers with Electrophilic Reagents.
- The Identification and Isolation of Reactive Thiols in Ricin A-Chain and Blocked Ricin Using 2-(4'-Maleimidylanilino)naphthalene-6-sulfonic Acid.
- Inactivation of the Human Cytomegalovirus Protease by Diisopropylfluorophosphate.
- Studies on the Status of Arginine Residues in Phospholipase A<->2 from Naja naja atra (Taiwan cobra) Snake Venom.
- Selective Reduction of the Intermolecular Disulfide Bridge in Human Glial Cell Lined-Derived Neurotrophic Factor Using Tris-(2-Carboxyethyl)Phosphine.
- Effects of Surface Hydrophobicity on the Structural Properties of Insulin.
- The Effects of in Vitro Methionine Oxidation on the Bioactivity and Structure of Human Keratinocyte Growth Factor.
- Posttranslational and Other Modifications:
- Effects of Enzyme Glycosylation on the Chemical Step of Catalysis, as Probed by Hydrogen Tunneling and Enthalpy of Activation.
- Profile Analysis of Oligosaccharides from Glycoproteins by PMP Labeling.
- Comparison of Chemical and Enzymatic Release Methods Using RP-HPLC and Mass Spectrometry.
- Positive Identification of Glycosylation Sites in Proteins and Peptides Using a Modified Beckman LF 3600 N-Terminal Protein Sequencer.
- Deamidation and Isoaspartate Formation during in Vitro Aging of a Recombinant Hepatitis E Vaccine Candidate.
- The Isolation and Characterization of Active Site Peptides in Lysyl Oxidase.
- Complement Activation in EDTA Blood/Plasma Samples May Be Caused by Coagulation Proteases.
- Disulfide-Linked Human Stem Cell Factor Dimer: Method of Identification and Molecular Comparison to the Noncovalent Dimer.
- Autocatalytic Reduction of a Humanized Antibody.
- Interactions of Protein with Ligands:
- Oxygen and Ascorbate Mediated Modification of a Recombinant Hemoglobin.
- Metal Activation and Regulation of E. coli Rnase H. Crystal Structure of Avian Sarcoma Virus Integrase with Bound Essential Cations.
- Multidimensional NMR Studies of an Exchangeable Apolipoprotein and Its Interactions with Lipids.
- NMR Methods for Analysis of CRALBP Retinoid Binding.
- Novel Method for Measuring the Binding Properties of the Site-Directed Mutants of the Proteins that Bind Hydrophobic Ligands: Application to Cellular Retinoic Acid Binding Proteins.
- A Strategy for Predicting the Ligand Binding Competence of Recombinant Orphan Nuclear Receptors Using Biophysical Characterization.
- Protein-Protein Interactions:
- Detection of Intra-Cellular Protein-Protein Intractions: Penicillin Interactive Proteins and Morphogene Proteins.
- Use of Synthetic Peptides in Mapping the Binding Sites for hsp70 in a Mitochondrial Protein.
- Interfacing Biomolecular Interaction Analysis with Mass Spectrometry and the Use of Bioreactive Mass Spectrometer Probe Tips in Protein Characterization.
- Transition-State Theory and Secondary Forces in Antigen-Antibody Complexes.
- Thermodynamic Investigation of Enzyme and Inhibitor Interactions with High Affinity.
- Development and Characterization of a Fab Fragment as a Surrogate for the IL-1 Receptor.
- Macromolecular Assemblies:
- Topogology of Membrane Proteins in Native Membranes Using Matrix-Assisted Laser Desorption Ionization/Mass Spectrometry.
- Role of D-Ser<V>46</V> in the P-type Calcium Channel Blocker, w-Agatoxin-TK.
- Involvement of Basic Amphiphilic a-Helical Domain in the Reversible Membrane Interaction of Amphitropic Proteins: Structural Studies by Mass Spectrometry, Circular Dichroism, and Nuclear Magnetic Resonance.
- One-Dimensional Diffusion of a Protein along a Single-Stranded Nucleic Acid.
- Metal-Dependent Structure and Self Association of the RAG1 Zinc-Binding Domain.
- Localizing Flexibility within the Target Site of DNA-Bending Proteins.
- Assembly of the Multifunctional EcoKI DNA Restriction Enzyme in Vitro.
- Three Dimensional Structure:
- Strategies for NMR Assignment and Global Fold Determinations Using Perdeuterated Proteins.
- <V>1</V>H-NMR Evidence for Two Buried ASN Side-Chains in the c-MYC-MAX Heterodimeric a-Helical Coiled-Coil.
- NMR Confirms the Presence of the Amino-Terminal Helix of Group II Phospholipase A<->2 in Solution.
- The Crystallographic Analysis of Glycosylation-Inhibiting Factor
- Structure of the D30N Active Site Mutant of FIV Proteinase Complexed with a Statine-Based Inhibitor.
- A Homology-Based Model of Juvenile Hormone Esterase from the Crop Pest, Heliothis virescens.
- Analysis of Linkers of Regular Secondary Structures in Proteins.
- Structural and Functiona Roles of Tyrosine-50 of Yeast Guanylate Kinase.
- Dynamics and Folding:
- Flexibility of Serine Protease in Nonaqueous Solvent.
- Internal Dynamics of Human Ubiquitin Revealed by <V>13</V>C-Relaxation Studies of Randomly Fractionally Labeled Protein.
- Detection of Protein Unfolding and Fluctuations by Native State Hydrogen Exchange.
- Laser Temperature Jump for the Study of Early Events in Protein Folding.
- Biophysical and Structural Analysis of Human Acidic Fibroblast Growth Factor.
- A Thermodynamic Analysis Discriminating Loop Backbone.
- The Equilibrium Ensemble of Conformational States in Staphylococcal Nuclease.
- An Evaluation of Protein Secondary Structure Prediction Algorithms.
- Biological and Chemical Design:
- Designing Water Soluble b-Sheet Peptides with Compact Structure.
- Engineering Secondary Structure to Invert Coenzyme Specificity in Isopropylmalate Dehydrogenase.
- A Method for Determining Domain Binding Sites in Proteins with Swapped Domains: Implications for bA3- and bB2- Crystallins.
- Complete Mutagenesis of the Gene Encoding TEM-1 b-Lactamase.
- -- Characterization of Truncated Kirsten-Ras Purified from Baculovirus Infected Insect Cells Indicates Heterogeneity due to N-terminal Processing and Nucleotide Dissociation.
- Isolation and Characterization of Multiple-Methionine Mutants of T4 Lysozyme with Simplified Cores.
- Synthesis of Alzheimer's (1-42) Ab-Amyloid Peptide with Preformed Fmoc-Aminoacyl Fluorides.
- Analysis of Racemization during "Standard" Solid Phase Peptide Synthesis: A Multicenter Study.
- Index.