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DNA microarrays. Volume 411, Part B, Databases and statistics /
Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist th...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam ; San Diego :
Elsevier/Academic Press,
�2006.
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| Colección: | Methods in enzymology ;
volume 411, pt. B. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
Tabla de Contenidos:
- Cover Page; Table of Contents; Contributors to Volume 411; Volumes in Series; Chapter 1: RNA Extraction for Arrays; Introduction; Blood as a Biological Specimen; Overview of the LeukoLOCK Procedure for Isolation of RNA from Whole Blood Samples; Use of Solid Tissues for Gene Expression Analysis; RNA Quality Measurements for Microarray Analysis; Conclusion; Acknowledgments; References; Chapter 2: Analyzing Micro-RNA Expression Using Microarrays; Introduction; Micro-RNAs Are Important Factors in Human Cancer; Focus of This Chapter; Microarray Platforms.
- MiRNA Preparation for Analysis on MicroarraysAnalysis of miRNA Microarray Results; Methods of Normalization for miRNA Microarray Experiments; Case Study: miRNA Microarray Expression Analysis of Human Lung and Placental Tissues; Scanning and Data Extraction; Sample Size Calculation; Calculation of Array-Specific Thresholds; Global Normalization; Statistical Differential Analysis; Hierarchical Clustering; Conclusion; Acknowledgments; References; Chapter 3: Troubleshooting Microarray Hybridizations; Introduction; General Considerations; Printing; Sample Preparation and Labeling.
- Background FluorescenceHybridization Quality Assessment; Concluding Remarks; Acknowledgments; Internet Resources; References; Chapter 4: Use of External Controls in Microarray Experiments; Introduction; Description and Availability of External Controls; Assessment of Array Performance Using External RNA Controls; Methods for Synthesis and Utilization of External RNA Controls; Evaluation of Data Analysis Methodology Using Spike-In Data Sets; Concluding Remarks; Acknowledgments; References; Chapter 5: Standards in Gene Expression Microarray Experiments; Introduction; Variability.
- A Digression: Traceability, Validation, and UncertaintyStandards in Traceability, Validation, and Uncertainty for DNA Microarray Gene Expression Profiles; Data Exchange Standards; Standards in the Gene Expression Process Model; The Future; References; Chapter 6: Scanning Microarrays: Current Methods and Future Directions; Introduction; Overview of the Scanning Process; User-Controlled Parameters; Instrumentation/Hardware Effects; Alternative Scanning Technologies Provide Advantages; Specific Considerations for Multiple Slide, Multiple Scanner, and/or Multiple Laboratory Experiments.
- ConclusionsAcknowledgments; References; Chapter 7: An Introduction to BioArray Software Environment; Introduction; Getting Started; The Basics of BASE; Working with BASE; References; Chapter 8: Bioconductor: An Open Source Framework for Bioinformatics and Computational Biology; Introduction: Bioconductor in Brief; Technical Details; Array Preprocessing; Addressing Multiple Comparisons; Conclusions: Data Analysis for High-Throughput Biology and Bioconductor; Acknowledgment; References; Chapter 9: TM4 Microarray Software Suite; Introduction; MADAM; Spotfinder; Spotfinder Protocol Description.