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Amyloid, prions, and other protein aggregates. Part C /
The ability of polypeptides to form alternatively folded, polymeric structures such as amyloids and related aggregates is being increasingly recognized as a major new frontier in protein research. This new volume of Methods in Enzymology along with Part C (volume 413) on Amyloid, Prions and other Pr...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam ; Boston :
Elsevier/Academic,
�2006.
|
| Colección: | Methods in enzymology ;
v. 413. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo Texto completo Texto completo |
Tabla de Contenidos:
- Cover; Copyright page; Table of Contents; Contributors to Volume 413; Preface; Volume in series; Chapter 1: Purification of Polyglutamine Proteins; Abstract; Introduction; Ataxin-3 Constructs Used; Manipulation of the Polyglutamine Tract Length Using Cassette Mutagenesis; Expression and Purification of Ataxin-3; Quality Control and Analysis of Purified Ataxin-3; Conclusion; References; Chapter 2: Preparation of Amyloid beta-Protein for Structural and Functional Studies; Abstract; Background; Theoretical and Practical Aspects of Amyloid Protein Preparation.
- Preparing Abeta for Biophysical and Biological StudyAcknowledgments; References; Chapter 3: Kinetics and Thermodynamics of Amyloid Assembly Using a High-Performance Liquid Chromatography-Based Sedimentation Assay; Abstract; Introduction; Disaggregation of Peptides; Preparation of Stocks of Aggregate Seeds; An HPLC Sedimentation Assay for Amyloid Transformations; Titration of Fibril Growing Ends; Elongation Kinetics; Nucleation Kinetics Analysis; Thermodynamics of Amyloid Fibril Elongation; Concluding Remarks; Acknowledgment; References.
- Chapter 4: Protein Aggregation Starting From The Native Globular StateAbstract; Introduction; Basic Features of Our Approach; Aggregation Mechanism of HypF-N; Aggregation Mechanism of Sso AcP; Conclusions; References; Chapter 5: Direct Observation of Amyloid Growth Monitored by Total Internal Reflection Fluorescence Microscopy; Abstract; Introduction; TIRFM; Direct Observation of Amyloid beta (1-40) Fibrils; Conclusion; Acknowledgments; References; Chapter 6: Characterization of Amyloid Structures at the Molecular Level by Solid State Nuclear Magnetic Resonance Spectroscopy; Abstract.
- IntroductionDetermination of Secondary Structure; Determination of Tertiary Structure; Determination of Quaternary Structure; Construction of Molecular Models Based on Solid State NMR Data; Sample Preparation for Solid State NMR; Acknowledgments; References; Chapter 7: Spin Labeling Analysis of Amyloids and Other Protein Aggregates; Abstract; Introduction; Application to Amyloids and Other Protein Aggregates; Outlook; Acknowledgments; References; Chapter 8: Hydrogen/Deuterium Exchange Mass Spectrometry Analysis of Protein Aggregates; Abstract; Introduction.
- Technological Considerations in H/D Exchange-MSH/D Exchange into Abeta(1-40) Fibrils; Achieving Higher Spatial Resolution by On-Line H/D Exchange-MS with Proteolysis; Proteolysis of Abeta(1-40) Amyloid Fibrils; Conclusions; Acknowledgments; References; Chapter 9: Hydrogen-Deuterium Exchange Analyzed by Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry and the HET-s Prion Model; Abstract; Introduction; Materials and Methods; Results; Future Perspective: Improving the Resolution of HXMS Experiments; Conclusion; Acknowledgments; References.