DNA microarrays /

Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist th...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Kimmel, Alan R. (Editor ), Oliver, Brian, 1959- (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego, California : Elsevier/Academic Press, �2006.
Colección:Methods in enzymology ; v. 410.
Methods in enzymology ; v. 411.
Temas:
DNA microarrays.
DNA.
Oligonucleotide Array Sequence Analysis > methods
DNA
Gene Expression Profiling > methods
Puces �a ADN.
ADN.
DNA microarrays
dna
dna libraries
dna probes
genexpressie
gene expression
rna
Nucleic Acids
Acceso en línea:Texto completo
Texto completo
Texto completo
Tabla de Contenidos:
  • Part A (v. 410) Array platforms and wet-bench protocols
  • Part B (v. 411) Databases and statistics.
  • Section I Array Platforms
  • Chapter 1: The Affymetrix GeneChipreg Platform: An Overview
  • Introduction
  • GeneChip Microarrays, a Flexible Platform
  • Array Manufacturing
  • Array Design
  • Target Preparation
  • GeneChip Instrument Components and Associated Assay Steps
  • Image and Data Analysis
  • Current Applications
  • Advancing the Future of Genomics
  • Acknowledgments
  • References
  • Chapter 2: The Agilent In Situ-Synthesized Microarray Platform
  • Introduction
  • Technology
  • Applications
  • Methods Descriptions
  • Array Design
  • Sample Isolation, Labeling, and Quality Control
  • Array Hybridization and Scanning
  • Data Extraction
  • Array Design
  • Process Description
  • Sample Isolation, Labeling, and Quality Control
  • Array Hybridization and Scanning
  • Data Extraction
  • References
  • Chapter 3: Illumina Universal Bead Arrays
  • Introduction
  • Material and Methods
  • Results and Discussion
  • Conclusion
  • Acknowledgments
  • References
  • Chapter 4: Microarray Oligonucleotide Probes
  • The Case for Oligonucleotide Probes
  • Considerations for Oligonucleotide Probe Design
  • Microarray Production and Hybridization Protocols
  • Practical Considerations in Probe Sequence Design, a Case Study
  • Employment and Postprocessing
  • Conclusion
  • Locations of Probe Sequence Target Regions: Discrimination of Highly Similar Targets
  • In Situ Synthesis vs Deposition of Presynthesized Oligonucleotides
  • Thermodynamic Modeling of Microarray Probe Hybridization
  • Outlook
  • Supplement
  • Acknowledgments
  • References
  • Chapter 5: Automated Liquid Handling and High-Throughput Preparation of Polymerase Chain Reaction-Amplified DNA for Microarray Fabrication
  • Introduction
  • Overview
  • Methods
  • Summary
  • Acknowledgments
  • References
  • Chapter 6: The Printing Process: Tips on Tips
  • Introduction
  • The Printing Process and Equipment
  • Printing Pin Technology Options
  • Critical Parameters and Troubleshooting
  • Quality Control Testing
  • Future Developments
  • Acknowledgment
  • References
  • Chapter 7: Making and Using Spotted DNA Microarrays in an Academic Core Laboratory
  • Introduction
  • Technologies and Services Provided by the Keck Microarray Resource
  • Generic Glass Slide Microarray Printing
  • Genomic Solutions OmniGrid 100 Microarrayer Print Settings
  • Materials and General Settings for cDNA and Oligonucleotide Printing on In-House PLL-Coated Slides
  • Quality Control Parameters for DNA Microarray Printing and Analysis Using Spotted Glass Slides
  • Labeling and Hybridization Protocols Employed by the Keck Microarray Resource
  • General Considerations Regarding RNA Samples, Labeling, and Hybridization Strategies
  • Indirect Amino-Allyl dUTP Target Labeling, Monofunctional Dye Conjugation, and cDNA and Oligonucleotide Array Hybridization Protocols
  • Genisphere 3DNA Dendrimer Labeling and Oligonucleotide Array Hybridization
  • tidtid6.

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