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245 0 0 |a RNA interference /  |c edited by David R. Engelke, John J. Rossi. 
260 |a San Diego :  |b Elsevier Academic Press,  |c �2005. 
300 |a 1 online resource (xxxvii, 453 pages, 13 unnumbered pages of plates) :  |b illustrations (some color). 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
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380 |a Handbook 
490 1 |a Methods in enzymology,  |x 0076-6879 ;  |v v. 392 
550 |a Made available through: Science Direct. 
504 |a Includes bibliographical references and indexes. 
505 0 |a Cover -- front cover -- copyright -- Table of Contents -- Contributors to Volume 392 -- Methods in Enzymology -- [1] Transgene-Induced RNA Interference as a Tool for Plant Functional Genomics -- [2] Constructs and Methods for Hairpin RNA-Mediated Gene Silencing in Plants -- [3] RNA Interference in Caenorhabditis elegans -- [4] High-Throughput RNA Interference Screens in Drosophila Tissue Culture Cells -- [5] Mechanistic Insights Aid Computational Short Interfering RNA Design -- [6] Novel Methods for Expressing RNA Interference in Human Cells -- [7] Delivery of Small Interfering RNA to Mammalian Cells in Culture by Using Cationic Lipid/Polymer-Based Transfection Reagents -- [8] Subcellular Distribution of Small Interfering RNA: Directed Delivery Through RNA Polymerase III Expression Cassettes and Localization by In Situ Hybridization -- [9] Viral Delivery of Recombinant Short Hairpin RNAs -- [10] Targeting Cellular Genes with PCR Cassettes Expressing Short Interfering RNAs -- [11] Use of Short Hairpin RNA Expression Vectors to Study Mammalian Neural Development -- [12] Analysis of T-Cell Development by Using Short Interfering RNA to Knock Down Protein Expression -- [13] Lentiviral Vector Delivery of Recombinant Small Interfering RNA Expression Cassettes -- [14] Paradigms for Conditional Expression of RNA Interference Molecules for Use Against Viral Targets -- [15] High-Throughput RNA Interference Strategies for Target Discovery and Validation by Using Synthetic Short Interfering RNAs: Functional Genomics Investigations of Biological Pathways -- [16] RNA Interference In Vivo: Toward Synthetic Small Inhibitory RNA-Based Therapeutics -- [17] Labeling and Characterization of Small RNAs Associated with the RNA Interference Effector Complex RITS -- [18] RNA Interference Spreading in C. elegans -- [19] Human Dicer: Purification, Properties, and Interaction with PAZ PIWI Domain Proteins -- [20] Delivery of siRNA and siRNA Expression Constructs to Adult Mammals by Hydrodynamic Intravascular Injection -- [21] Analysis of Short Interfering RNA Function in RNA Interference by Using Drosophila Embryo Extracts and Schneider Cells -- [22] Use of RNA Polymerase II to Transcribe Artificial MicroRNAs -- [23] Adeno-Associated Virus Vectors for Short Hairpin RNA Expression -- [24] Simple, Robust Strategies for Generating DNA-Directed RNA Interference Constructs -- Author Index -- Subject Index -- Last Page. 
520 |a The newest volume in the critically acclaimed laboratory standard, Methods in Enzymology. 
546 |a English. 
650 0 |a RNA  |v Handbooks, manuals, etc. 
650 0 |a RNA editing  |v Handbooks, manuals, etc. 
650 0 |a Gene silencing  |v Handbooks, manuals, etc. 
650 0 |a Small interfering RNA  |v Handbooks, manuals, etc. 
650 0 |a RNA interference. 
650 0 |a RNA. 
650 0 |a RNA editing. 
650 0 |a Gene silencing. 
650 2 |a RNA Interference  |0 (DNLM)D034622 
650 2 |a RNA  |0 (DNLM)D012313 
650 6 |a ARN.  |0 (CaQQLa)201-0024711 
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700 1 |a Engelke, David R.  |q (David Ross),  |e editor. 
700 1 |a Rossi, John J.,  |e editor. 
776 0 8 |i Print version:  |t RNA interference.  |d San Diego : Elsevier Academic Press, �2005  |z 0121827976  |w (OCoLC)57676191 
830 0 |a Methods in enzymology ;  |v v. 392.  |x 0076-6879 
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