Regulators of G-protein signaling. Volume 389-390, Part A-B /

This volume is an in-depth treatment of G-Protein Signaling, Part A, and will cover general methods of analysis of RGS protein analysis, including Expression and post-translational modification, Assays of GAP activity and allosteric control, Electrophysiological methods and RGS-insensitive Ga subuni...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Siderovski, David P. (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego, California : Elsevier Academic Press, 2004.
Colección:Methods in enzymology ; volume 389.
Methods in enzymology ; volume 390.
Temas:
G proteins.
Intracellular Signaling Peptides and Proteins
GTP-Binding Protein Regulators
RGS Proteins > analysis
GTP-Binding Proteins
Prot�eines G.
G proteins
G-prote�inen.
Signalen.
Acceso en línea:Texto completo
Texto completo
Texto completo
Tabla de Contenidos:
  • Cover
  • Table of Contents
  • Contributors to Volume 389
  • Preface
  • Volumes in Series
  • Section I: General Methods of RGS Protein Analysis Subsection A. Expression and Post-translational Modification
  • Chapter 1. Quantitative Real-Time Polymerase Chain Reaction Measurement of Regulators of G-Protein Signaling mRNA Levels in Mouse Tissues
  • Chapter 2. Regulation of Chemokine-Induced Lymphocyte Migration by RGS Proteins
  • Chapter 3. Role of Palmitoylation in RGS Protein Function
  • Subsection B: Assays of RGS Box Activity and Allosteric Control
  • Chapter 4. Fluorescence-Based Assays for RGS Box Function
  • Chapter 5. Application of RGS Box Proteins to Evaluate G-Protein Selectivity in Receptor-Promoted Signaling
  • Chapter 6. Allosteric Regulation of GAP Activity by Phospholipids in Regulators of G-Protein Signaling
  • Chapter 7. Assays of RGS Protein Modulation by Phosphatidylinositides and Calmodulin
  • Subsection C. Electrophysiological Methods and RGS-Insensitive Galpha Subunits
  • Chapter 8. Analyses of RGS Protein Control of Agonist- Evoked Ca2+ Signaling
  • Chapter 9. Measuring the Modulatory Effects of RGS Proteins on GIRK Channels
  • Chapter 10. Assays for G-Protein-Coupled Receptor Signaling Using RGS-Insensitive Galpha Subunits
  • Chapter 11. Use of RGS-Insensitive Galpha Subunits to Study Endogenous RGS Protein Action on G-Protein Modulation of N-Type Calcium Channels in Sympathetic Neurons
  • Chapter 12. Endogenous RGS Proteins Regulate Presynaptic and Postsynaptic Function: Functional Expression of RGS-Insensitive Galpha Subunits in Central Nervous System Neurons
  • Subsection D. Mouse Models of RGS Protein Action
  • Chapter 13. In Situ Hybridization Analysis of RGS mRNA Regulation and Behavioral Phenotyping of RGS Mutant Mice
  • Chapter 14. RGS-Insensitive G-Protein Mutations to Study the Role of Endogenous RGS Proteins
  • Subsection E. Methods of RGS Protein Inhibition
  • Chapter 15. Ribozyme- and siRNA-Mediated Suppression of RGS-Containing RhoGEF Proteins
  • Chapter 16. Structure-Based Design, Synthesis, and Activity of Peptide Inhibitors of RGS4 GAP Activity
  • Chapter 17. Yeast-Based Screening for Inhibitors of RGS Proteins
  • Section II: G-Protein Regulators of Model Organisms
  • Chapter 18. Genetic Analysis of RGS Protein Function in Caenorhabditis elegans
  • Chapter 19. Purification and In Vitro Functional Analysis of the Arabidopsis thaliana Regulator of G-Protein Signaling-1
  • Chapter 20. AtRGS1 Function in Arabidopsis thaliana
  • Chapter 21. Identification and Functional Analysis of the Drosophila Gene loco
  • Chapter 22. Analysis of the Roles of Pins and Heterotrimeric G Proteins in Asymmetric Division of Drosophila Neuroblasts
  • Chapter 23. Mathematical Modeling of RGS and G-Protein Regulation in Yeast
  • Chapter 24. Identification of Yeast Pheromone Pathway Modulators by High-Throughput Agonist Response Profiling of a Yeast Gene Knockout Strain Collection
  • Author Index
  • Subject Index
  • Last Page.

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