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|a Atlas of cilia bioengineering and biocomputing /
|c editors, Richard Mayne, Jaap den Toonder.
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|a Gistrup, Denmark :
|b River Publishers,
|c [2018]
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|a 1 online resource
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|a River publishers series in biomedical engineering.
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|a Includes bibliographical references and index.
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|a Front Cover; Half Title Page; RIVER PUBLISHERS SERIES IN BIOMEDICALENGINEERING; Title Page; Copyright Page; Contents; Editor Foreword; List of Contributors; List of Figures; List of Tables; List of Abbreviations; PART I : Biology; Chapter 1 -- Biological Preliminaries for Cilia Study; 1.1 Introduction; 1.2 Primary Cilia; 1.3 Motile Cilia; 1.3.1 Structure; 1.3.2 The Ciliary Beat; References; Chapter 2 -- Genetic Engineering of Ciliated Cells; 2.1 Introduction; 2.2 Cell Selection and Growth; 2.2.1 Cells with Primary Cilia; 2.2.2 Cells with Motile Cilia; 2.3 Transfection of Cells.
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|a 2.4 RNA Interference2.5 Fluorescent Protein Labeling; 2.6 Optogenetics; 2.7 Proximity-Dependent Biotinylation (BioID); 2.8 Tandem Affinity Purification (TAP); 2.9 Genome Editing Using TALENs, Zn Finger Nucleases, CRISPR/Cas9, and Base Editing; Acknowledgments; References; Chapter 3 -- Programming Ciliary Object Manipulation; 3.1 Introduction; 3.2 Sorting by Paramecium Cilia; 3.3 Reprogramming Paramecium Cilia; Reference; PART II : Engineering; Chapter 4 -- Robotic Cilia for Autonomous Parallel Distributed Sorting Platforms; 4.1 Introduction; 4.2 Engineering Sorting Ciliary Platforms; References.
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|a Chapter 5 -- Artificial Pneumatic Cilia5.1 Introduction; 5.2 Base Actuated Pneumatic Cilia; 5.3 Body Actuated Pneumatic Cilia; 5.4 Conclusion; Acknowledgments; References; Chapter 6 -- Blinking-Vortex Inspired Mixing with Cilia; 6.1 Cilia Actuation Scheme; 6.2 Experiments and Simulations; 6.3 Results; References; Chapter 7 -- Magnetic Thin-film Cilia for Microfluidic Applications; 7.1 Introduction; 7.2 Fabrication of Magnetic Cilia; 7.3 Fluid Transport by An Array of Beating Magnetic Cilia; 7.4 Kinematics of Magnetic Cilia; 7.4.1 Counter-Clockwise Rotation of Magnetic Field.
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|a 7.4.2 Clockwise Rotation of Magnetic Field7.5 Metachronal Motion; 7.6 Microfluidic Applications of Artificial Cilia; 7.6.1 Microfluidic Mixing; 7.6.2 Bacteria Capture; 7.7 Summary; Acknowledgments; References; Chapter 8 -- Artificial Cilia: Fabrication, Actuation, and Flow Generation; 8.1 Introduction; References; PART III : Theoretical and Computer Modelling; Chapter 9 -- Numerical Simulations of Fluid Transport by Magnetically Actuated Artificial Cilia; 9.1 Introduction; References; Chapter 10 -- Modeling Gel-based Artificial Cilia; 10.1 Introduction.
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|a 10.2 Designing Chemo-responsive Cilial Systems10.2.1 Methodology: Gel Lattice Spring Model; 10.2.2 Self-oscillating Cilia; 10.3 Photo-responsive Gel-Fiber Systems; 10.4 Capturing Hydrodynamic Interactions in Complex Fluids; 10.4.1 Methodology; 10.4.2 Utilizing Oscillating Rods to "Catch and Release" Targeted Nanoparticles; 10.5 Artificial Cilia Based on Biological Aptamers; 10.5.1 Methodology; 10.5.2 Catch and Release with Aptamer-mimicking Cilia; 10.6 Conclusions; References; Index; About the Editors; Back Cover.
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|a Online resource; title from digital title page (viewed on December 04, 2018).
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|a Cilia are microscopic finger-like cell-surface organelles possessed by a great many eukaryotic organisms, including humans, whose purposes include generating local fluid movements via rhythmic whip-like beating and environmental sensing. Despite intense research efforts since their discovery by van Leeuwenhoek in the 1670's, several key questions regarding ciliary functions, experimental manipulation and in silico imitation remain unanswered. Major justifications for cilia research lie in their involvement in various forms of human disease (ciliopathies) and their ability to instantiate decentralised, asynchronous sensorial-actuation of adjacent matter through modulation of beating characteristics. Further elucidation of these characteristics, which is a problem requiring the combined expertise of mathematicians, computer scientists, engineers and life scientists, will lead to novel biomedical therapies, creation of 'smart' actuating surfaces for microfluidics/lab-on-chip applications and a greater understanding of fluid mechanics in real-world scenarios. This lavishly-illustrated anthology presents recent advances in the fields of ciliary investigation, manipulation, emulation, mimesis and modelling from key researchers in their fields: its goal is to explain the state-of-the-art in cilia bioengineering and bio-computation in a uniquely creative, accessible manner, towards encouraging further transdisciplinary work in the field as well as educating a broad spectrum of scientists and lay people. The volume is split into three distinct but interwoven themes: 1. Biology: Biological preliminaries for the study of cilia; the state-of-the-art in genetic engineering of ciliated cells for biomedical purposes; reprogramming of cilia dynamics in live cells. 2. Engineering: Creation of macro cilia robots for object sorting applications; pneumatic cilia for the optimization of fluid motion; electrostatic, magnetic and MEMS cilia for microfluidic mixing; reviews in artificial cilia fabrication, actuation and flow induction methods. 3. Numerical and computational modelling. Analyses of thin film cilia for `lab on chip' microfluidic mixing applications; modelling of gel-based artificial cilia towards simulating dynamic behaviors of responsive cilia layers in complex fluids across a wide range of potential applications.
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|a Richard Mayne, Jaap den Toonder
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|a Cilia and ciliary motion
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