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Concepts and models for drug permeability studies : cell and tissue based In vitro culture models /
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam :
Elsevier Ltd.,
2016.
|
| Colección: | Woodhead Publishing series in biomedicine ;
no. 79. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Related titles; Concepts and Models for Drug Permeability Studies; Copyright; Contents; List of contributors; List of figures; List of tables; 1
- Introduction; 1.1 Introduction; References; 2
- Importance and applications of cell- and tissue-based in vitro models for drug permeability screening in early stages o ... ; 2.1 Introduction; 2.2 General considerations; 2.3 Drug transport; 2.3.1 Transport mechanisms; 2.4 Permeability-absorption models; 2.4.1 Physicochemical methods; 2.4.1.1 Physicochemical factors; 2.4.1.2 Immobilized artificial membrane chromatography
- 2.4.1.3 Parallel artificial membrane permeability assay2.4.2 In vitro cell and tissue methods; 2.4.2.1 Cell-based methods; 2.4.2.2 Tissue-based methods; Diffusion chambers; Franz cells; Everted sacs; Isolated membrane vesicles; 2.5 Methods for permeability calculation; 2.6 Standardization of protocols for in vitro methods; 2.7 The "three Rs" principle; 2.8 Biosecurity systems; References; 3.1
- Cell-based in vitro models for buccal permeability studies; 3.1.1 Introduction; 3.1.2 Physiology of the buccal mucosa; 3.1.3 Different in vitro models; 3.1.3.1 Hamster cheek pouch cells
- 3.1.3.2 TR146 cell line3.1.3.2.1 Protocol; Cell culture conditions; Permeability studies; 3.1.3.3 Human oral keratinocytes; 3.1.3.3.1 Protocol; Oral keratinocyte culture conditions; Culturing on dead de-epidermized dermis (DDED); Permeability studies; 3.1.3.4 MatTek EpiOral™; 3.1.3.4.1 Protocol; Cell culture conditions; Permeability studies; 3.1.4 Conclusions; References; 3.2
- Cell-based in vitro models for gastric permeability studies; 3.2.1 The stomach as a natural barrier to absorption; 3.2.2 Gastric drug delivery; 3.2.2.1 Molecular absorption in the stomach
- 3.2.2.2 Physicochemical factors mediating stomach absorptive permeability3.2.3 Cellularized models of gastric permeability; 3.2.3.1 Protocol for establishing cellularized artificial models of the gastric wall; 3.2.4 Conclusions; Acknowledgments; References; 3.3
- Cell-based in vitro models for intestinal permeability studies; 3.3.1 Anatomy and physiology of human small intestine; 3.3.1.1 Stromal-epithelial cross-talk; 3.3.2 Mechanisms of transport; 3.3.3 Intestinal barriers; 3.3.4 Intestinal in vitro models; 3.3.4.1 Caco-2 model; 3.3.4.1.1 Accelerated Caco-2 models
- 3.3.4.1.2 Alternatives to the Caco-2 model3.3.4.2 Caco-2/HT29-MTX model; 3.3.4.3 Caco-2/Raji B model; 3.3.4.4 Caco-2/HT29-MTX/Raji B; 3.3.4.5 Novel 3D in vitro models; 3.3.5 Validation studies; 3.3.6 Conclusions; References; 3.4
- Cell-based in vitro models for nasal permeability studies; 3.4.1 Introduction; 3.4.2 Nasal primary cell culture models; 3.4.2.1 Sampling approaches and procedures; 3.4.2.2 HNE cell preparation and culture initiation; 3.4.3 Immortalized nasal cell lines; 3.4.3.1 RMPI 2650 cell line; 3.4.3.2 BT cell line; 3.4.3.3 Human lung carcinoma cell line