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Methods in Bioengineering : Microdevices in Biology and Medicine.
Offering a practical look into the field, this volume presents the science behind microscale device design and the engineering of its fabrication. Supported with dozens of full-color illustrations, this book offers you clear, step-by-step methods for the cell capture from whole blood, high-throughpu...
| Clasificación: | Libro Electrónico |
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| Autor principal: | |
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Norwood :
Artech House,
2009.
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| Colección: | Methods in bioengineering.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Methods in Bioengineering: Microdevices in Biology and Medicine; Contents; Preface; Chapter 1 Immunoaffinity Capture of Cells from Whole Blood; 1.1 Introduction; 1.2 Experimental Design; 1.3 Materials; 1.4 Methods; 1.4.1 Device fabrication; 1.4.2 Fluidic port punching; 1.4.3 Surface modification; 1.4.4 Cell capture; 1.4.5 Injecting blood into cassette; 1.4.6 Washing noncaptured cells with PBS; 1.4.7 Postcapture processing; 1.4.8 Immunofluorescence staining; 1.4.9 Giemsa staining protocol; 1.4.10 Cell lysis for genomic applications; 1.5 Data Acquisition, Anticipated Results, and Interpretation.
- 1.6 Discussion and Commentary1.7 Application Notes; 1.8 Summary Points; Acknowledgments; References; Chapter 2 Dynamic Gene-Expression Analysis in a Microfluidic Living Cell Array (mLCA); 2.1 Introduction; 2.2 Materials; 2.2.1 Reagents; 2.2.2 Fabrication facilities; 2.2.3 Imaging equipment; 2.2.4 Perfusion components; 2.3 Methods; 4.