Single molecule biophysics and poisson process approach to statistical mechanics /

This is an overview of single molecule physics, the study of both equilibrium and non-equilibrium properties at the single molecule level. It begins with an introduction to this fascinating science and includes a chapter on how to build the most popular instrument for single molecule biophysics, the...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: Sarkar, Susanta K. (Autor)
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Rafael [California] (40 Oak Drive, San Rafael, CA, 94903, USA) : Morgan & Claypool Publishers, [2016]
Colección:IOP (Series). Release 2.
IOP concise physics.
Temas:
Molecular Biology > methods.
Biophysics.
Instruments and instrumentation engineering.
SCIENCE / Life Sciences / Biophysics.
TECHNOLOGY & ENGINEERING / Measurement.
Biomolecules.
Biomolecules > Analysis.
Biophysics > methods.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Preface
  • 1. Single molecule biophysics
  • 1.1. Introduction
  • 1.2. A brief history of single molecule study
  • 1.3. Single molecule methods
  • 1.4. Single molecule data analysis
  • 1.5. Brief overview of thermodynamics and statistical mechanics
  • 1.6. Future outlook
  • 1.7. Conclusion
  • 2. The total internal reflection fluorescence microscope
  • 2.1. Introduction
  • 2.2. Design considerations
  • 2.3. Microscope frame and associated parts
  • 2.4. Microscope objectives
  • 2.5. Detection path and camera
  • 2.6. Excitation path
  • 2.7. Computer, computer software, and other computing hardware
  • 2.8. Experimental protocol for slide preparation
  • 2.9. Specifications of the TIRF microscope
  • 2.10. Objective-type TIRFM
  • 2.11. Multichannel detection and FRET
  • 2.12. Circular dichroism spectroscopy and microscope enclosure
  • 2.13. Acknowledgments
  • 3. Poisson process approach to statistical mechanics
  • 3.1. Introduction
  • 3.2. Diffusion
  • 3.3. Inter-conversion of the states of molecules
  • 3.4. Photon statistics from lasers
  • 3.5. Statistics of fluorescent centers on a surface
  • 3.6. Location of stars in the sky
  • 3.7. Quantifying the heterogeneity of the locations of cosmic ray sources
  • 3.8. Conclusion
  • 3.9. Acknowledgments
  • Appendices.
  • A. Building a total internal reflection fluorescence microscope: parts, vendors, and costs
  • B. The Poisson process.

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