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Total internal reflection fluorescence (TIRF) and evanescence microscopies /
This book offers a complete presentation of the physics, math, and experimental setups for both TIRF and related evanescence microscopies. It covers evanescence in both fluorescence excitation or emission. It also discusses, in detail, the theory, setups, and practical biological/biochemical applica...
| Clasificación: | Libro Electrónico |
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| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Bristol [England] (Temple Circus, Temple Way, Bristol BS1 6HG, UK) :
IOP Publishing,
[2022]
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| Colección: | IOP (Series). Release 22.
Biophysical Society-IOP series. IOP ebooks. 2022 collection. |
| Temas: | |
| Acceso en línea: | Texto completo |
| Sumario: | This book offers a complete presentation of the physics, math, and experimental setups for both TIRF and related evanescence microscopies. It covers evanescence in both fluorescence excitation or emission. It also discusses, in detail, the theory, setups, and practical biological/biochemical applications for combinations of evanescence microscopies with other optical techniques such as polarization, photobleaching, correlation spectroscopy, scattering, image enhancement, optical force, two-photon, energy transfer, structured illumination, scanning, quenching, and single molecule detection. Physical and qualitative discussions augment the rigorous math, making the book accessible and interesting to a wide range of audiences with backgrounds from biology to chemistry to physics. The book also contains useful step-by-step guides to building, modifying, and aligning TIRF microscopy systems for specialized purposes. Part of Biophysical Society-IOP series. |
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| Notas: | "Version: 20220901"--Title page verso. |
| Descripción Física: | 1 online resource (various pagings) : illustrations (some color). Also available in print. |
| Público: | Researchers who work with TIRF and near field optics as a standard technique, and those who want to develop the techniques farther. |
| Bibliografía: | Includes bibliographical references. |
| ISBN: | 9780750333511 9780750333504 |