Introduction to pharmaceutical biotechnology. Volume 1, Basic techniques and concepts /

Animal biotechnology is a broad field including polarities of fundamental and applied research, as well as DNA science, covering key topics of DNA studies and its recent applications. In Introduction to Pharmaceutical Biotechnology, DNA isolation procedures followed by molecular markers and screenin...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autores principales: Bhatia, Saurabh (Autor), Goli, Divakar (Autor)
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Bristol [England] (Temple Circus, Temple Way, Bristol BS1 6HG, UK) : IOP Publishing, [2018]
Colección:IOP (Series). Release 5.
IOP expanding physics.
Temas:
Pharmaceutical biotechnology.
Biopharmaceutics.
Drug Therapy > trends.
Drug Industry > trends.
Genetic Phenomena.
Genetic engineering.
SCIENCE / Life Sciences / Biophysics.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • 1. History, scope and development of biotechnology
  • 1.1. Introduction
  • 1.2. Branches of biotechnology
  • 1.3. Biotechnology and its various stages of development
  • 1.4. Scope and importance of biotechnology
  • 1.5. Biotechnology techniques
  • 1.6. Applications of biotechnology
  • 1.7. Biotech research : 2015-2016
  • 2. Modern DNA science and its applications
  • 2.1. Introduction
  • 2.2. The Human Genome Project
  • 2.3. DNA synthesis begins at replication origins
  • 2.4. Gene expression
  • 2.5. Structure of DNA
  • 2.6. DNA replication
  • 2.7. DNA supercoiling
  • 2.8. Repair and recombination
  • 2.9. Types of DNA damage
  • 2.10. DNA recombination
  • 2.11. DNA isolation is the commencement of molecular marker analysis
  • 2.12. Types of molecular markers
  • 2.13. Genomic library screening methods
  • 2.14. Triplex DNA, TFOS, PNAs, RNA-DNA hybrids and DSRNA/RNAI
  • 2.15. Isolation, sequencing and synthesis of genes
  • 2.16. Synthesis of genes
  • 2.17. Genomics and proteomics research
  • 3. Introduction to genetic engineering
  • 3.1. Introduction
  • 3.2. Gene transfer technologies
  • 3.3. Plasmids
  • 3.4. Different hosts and protein expression technologies
  • 3.5. Gene cloning
  • 3.6. Transfection methods and transgenic animals
  • 3.7. Applications of genetic engineering in biotechnology
  • 3.8. Mammalian cell line characterization
  • 3.9. In vitro fertilization (IVF) and embryo transfer in humans and domestic animals
  • 3.10. IVF in humans and embryo transfer
  • 3.11. Embryo transfer (ET) in humans
  • 3.12. Superovulation, IVF and embryo culture in farm animals
  • 3.13. ET in cattle
  • 4. Applications of stem cells in disease and gene therapy
  • 4.1. Introduction
  • 4.2. Types of gene therapy
  • 4.3. Gene therapy strategies
  • 4.4. Methods of gene therapy
  • 4.5. Gene therapy approaches
  • 4.6. Vectors for gene therapy
  • 4.7. Target sites for gene therapy
  • 4.8. Gene therapy strategies for cancer
  • 4.9. Gene therapy for AIDS
  • 4.10. Oligonucleotide therapies : antigene and antisense therapy
  • 4.11. Antisense oligonucleotides as therapeutic agents
  • 4.12. Chimeric oligonucleotides in gene correction
  • 4.13. Aptamers as therapeutic agents
  • 4.14. Ribozymes as therapeutic agents
  • 4.15. The future of gene therapy
  • 4.16. Stem cell research
  • 5. Transgenic animals in biotechnology
  • 5.1. Introduction
  • 5.2. Major objectives of gene transfer
  • 5.3. Cloning vectors
  • 5.4. Efficient and versatile mammalian virus vectors
  • 5.5. Mammalian artificial chromosome (MAC) vectors for somatic gene therapy
  • 5.6. DNA constructs
  • 5.7. Transfection methods : an approach towards mammalian cell transfection
  • 5.8. Xenopus oocytes as a heterologous expression system
  • 5.9. ES cell-mediated gene transfer
  • 5.10. Targeted gene transfer or gene therapy in mammals
  • 5.11. Transgene integration, organization and expression
  • 5.12. Transgene recovery in mammalian cells
  • 5.13. Cloned protein expression in mammalian cells.

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