Microbial Biotechnology in the Laboratory and Practice Theory, Exercises, and Specialist Laboratories.

This book examines fundamental issues in microbial biotechnology, such as microorganism culturing and uses in industry and environmental protection. It details modern analytical techniques, known as omics, as well as digital techniques used to record adverse changes in the environment resulting from...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: Długoński, Jerzy
Formato: Electrónico eBook
Idioma:Inglés
Publicado: La Vergne : Jagiellonian University Press, 2022.
Temas:
Microbial biotechnology.
Biotechnologie microbienne.
Microbial biotechnology
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Intro
  • Table of contents
  • Foreword
  • Foreword to the handbook Microbiological Biotechnology. Exercises and specialist laboratories from 1997
  • 1. Methods of screening, culturing, improvement and storage of microorganisms of industrial...
  • 1.1. General characteristics of microorganisms used in biotechnological processes
  • 1.2. Screening of microorganisms useful in microbiological processes
  • 1.2.1. Suitability of various environments for isolation of microorganisms used in industrial processes
  • 1.2.2. Soil as a source of potential producers of biologically active compounds
  • 1.2.3. Microbial screening
  • 1.2.4. Soil of polluted environments as a source of microorganisms used in environmental protection processes
  • 1.3. Methods of culturing and stabilisation of microorganisms under aerobic conditions (including bioreactors and immobilisation in gels)
  • 1.3.1. Batch culture
  • 1.3.2. Fed-batch culture
  • batch with continuous dosing of nutrient to the fermenter
  • 1.3.3. Continuous culture
  • 1.3.4. Bioreactors for submerged cultures
  • 1.3.5. Stabilisation of microorganisms by immobilisation
  • 1.4. Microorganisms culture in anaerobic conditions
  • 1.5. Fungal protoplasts: release, properties and application
  • 1.6. Strain development
  • mutagenesis, fusion and electroporation of protoplasts
  • 1.7. Storage of industrial strains
  • Literature
  • 2. Fundamentals of modern analytical techniques used in microbial biotechnology and related...
  • 2.1. Confocal, fluorescent microscopy and spectrofluorimetry
  • 2.1.1. Fluorescence phenomenon
  • 2.1.2. Spectrofluorimetry
  • 2.1.3. Fluorescent and confocal microscopy
  • comparison
  • 2.1.4. Autofluorescence and fluorescent markers
  • 2.1.5. Fluorescent proteins
  • 2.2. Isotopic techniques (radioactive isotopes)
  • 2.3. Chromatography
  • 2.3.1. Basic parameters measured in chromatography
  • 2.3.2. Liquid chromatography
  • 2.3.3. Gas chromatography
  • 2.4. Mass spectrometry
  • 2.4.1. Principle of mass spectrometer operation
  • 2.4.2. Ion sources and ion types in mass spectrometry
  • 2.4.3. Mass analysers
  • 2.4.4. Basic scanning modes
  • 2.4.5. Ion detection
  • 2.4.6. Applications of mass spectrometry
  • 2.5. Atomic absorption spectroscopy
  • 2.6. Modern digital techniques used to record changes in the environment
  • 2.6.1. Satelitte imagery in analyzing historical and last landuse
  • 2.6.2. Principles of detection selected remote sensing systems
  • 2.6.3. Airplane scanning in terrain measurement
  • Literature
  • 3. Determining the taxonomic affiliation of microorganisms
  • 3.1. Genotypic techniques for differentiation and identification of bacteria
  • 3.1.1. Isolation from the soil and identification of anaerobes by multiplex PCR
  • 3.1.2. Taxonomic classification of the genus Streptomyces based on PCR 16S rRNA method
  • 3.2. Fungi of the phyla Mucoromycota, Ascomycota and Basidiomycota
  • morphological, biochemical features and genetic analysis

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