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| LEADER |
00000cam a2200000Mu 4500 |
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EBSCO_on1231609943 |
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OCoLC |
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20231017213018.0 |
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m o d |
| 007 |
cr cnu---unuuu |
| 008 |
210116s2020 xx o ||| 0 eng d |
| 040 |
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|a EBLCP
|b eng
|c EBLCP
|d UKAHL
|d N$T
|d OCLCF
|d OCLCO
|d K6U
|d OCLCQ
|d OCLCO
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| 020 |
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|a 1774078945
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| 020 |
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|a 9781774078945
|q (electronic bk.)
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| 035 |
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|a (OCoLC)1231609943
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| 050 |
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|a QP519.9.I43
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| 082 |
0 |
4 |
|a 660.6
|2 23
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| 049 |
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|a UAMI
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| 245 |
0 |
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|a Overview of blotting techniques
|h [electronic resource].
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| 260 |
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|a Ashland :
|b Delve Publishing,
|c 2020.
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| 300 |
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|a 1 online resource (255 p.)
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| 500 |
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|a Description based upon print version of record.
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|a Cover -- Copyright -- DECLARATION -- ABOUT THE EDITORS -- TABLE OF CONTENTS -- List of Contributors -- List of Abbreviations -- Preface -- Chapter 1 Western Blotting-the Works -- 1.1 Concept -- 1.2 The Details -- 1.3 General Protocol For Western Blotting -- 1.4 Troubleshooting -- 1.5 Advances In Western Blot -- 1.6 Final Word -- 1.7 References -- Chapter 2 Southern Blotting and Associated Techniques -- 2.1 Introduction -- 2.2 Interesting Insights: The Role of Dr. Southern In Science -- 2.3 Technology Types -- 2.4 The Southern Blotting Principle -- 2.5 Recent Alterations To Southern Blotting
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|a 2.6 Dna Pre-Preparation Before Southern Blotting -- 2.7 Techniques For Dna Blotting -- 2.8 The Southern Blot Prehybridization -- 2.9 Washing Steps and Hybridization -- 2.10 Role of Southern Blotting In Identification of Clones -- 2.11 Polymorphism Analysis of Restriction Fragment Length -- 2.12 Final Words -- 2.13 References -- Chapter 3 Northern Blotting: A Brief Overview -- 3.1 Introduction -- 3.2 The Procedure In Brief -- 3.3 Applications Of The Northern Blotting Technique -- 3.4 Drawbacks Of The Northern Blotting Technique (Taniguchi Et Al., 2001 -- Karen Reue, 1998)
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| 505 |
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|a 3.5 The Reverse Northern Blot Technique -- 3.6 The Northwestern Blot -- 3.7 Summary -- 3.8 References -- Chapter 4 Eastern Blotting -- 4.1 Outline -- 4.2 Case Study 1: Eastern Blotting For A Herb Vs. Tlc (Thin Layer Chromatography) -- 4.3 Case Study 2: Eastern Blotting For Bioactive Compounds -- 4.4 References -- Chapter 5 Misleading Westerns: Common Quantification Mistakes In Western Blot Densitometry And Proposed Corrective Measures -- Abstract -- Introduction -- Materials And Methods -- Results -- Discussion -- Conclusions -- Data Availability -- Conflicts Of Interest -- Acknowledgments
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| 504 |
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|a References -- Chapter 6 Application of Monoclonal Antibodies Against Bioactive Natural Products: Eastern Blotting And Preparation of Knockout Extract -- Abstract -- Introduction -- Preparation of Mab Against Natural Products -- Conclusions -- References -- Chapter 7 Evaluating Strategies To Normalise Biological Replicates of Western Blot Data -- Abstract -- Introduction -- Materials And Methods -- Results -- Discussion -- Supporting Information -- Acknowledgments -- Funding Statement -- References -- Chapter 8 The Design of A Quantitative Western Blot Experiment -- Abstract -- Introduction.
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| 505 |
8 |
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|a Careful Experimental Design Produces Reliable And Reproducible Data -- Sample Preparation -- Determine The Linear Dynamic Range of Protein Loading -- Determine The Appropriate Reference Signal For Data Normalization -- Data Analysis: The Background Subtraction Problem -- Computational Analysis of Densitometric Data -- Conclusions -- Abbreviations -- Conflict Of Interests -- References -- Chapter 9 Characterization Of Nora Virus Structural Proteins Via Western Blot Analysis -- Abstract -- Introduction -- Materials And Methods -- Discussion -- Conclusion -- Competing Interests
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| 500 |
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|a Authors' Contributions.
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| 590 |
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|a eBooks on EBSCOhost
|b EBSCO eBook Subscription Academic Collection - Worldwide
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| 650 |
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|a Immunoblotting.
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| 650 |
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|a Western immunoblotting.
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| 650 |
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|a Macromolecules.
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| 650 |
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|a Technique des immunoempreintes.
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| 650 |
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|a Buvardage de western.
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| 650 |
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|a Immunoblotting
|2 fast
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| 650 |
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|a Macromolecules
|2 fast
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| 650 |
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|a Western immunoblotting
|2 fast
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| 700 |
1 |
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|a Sripathi, Shiv Sanjeevi.
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| 700 |
1 |
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|a Pandey, Prerna.
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| 776 |
0 |
8 |
|i Print version:
|a Sripathi, Shiv Sanjeevi
|t Overview of blotting techniques
|d Ashland : Delve Publishing,c2020
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| 856 |
4 |
0 |
|u https://ebsco.uam.elogim.com/login.aspx?direct=true&scope=site&db=nlebk&AN=2725292
|z Texto completo
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| 938 |
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|a Askews and Holts Library Services
|b ASKH
|n AH38250116
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| 938 |
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|a ProQuest Ebook Central
|b EBLB
|n EBL6453533
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| 938 |
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|a EBSCOhost
|b EBSC
|n 2725292
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| 994 |
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|a 92
|b IZTAP
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