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Surface plasmon resonance /
SPR is real-time, label-free measurements of binding kinetics and affinity. This has distinct advantage over radioactive or fluorescent labeling methods, in terms of 1) ligand-analyte binding kinetics, that can be probed without the costly and time-consuming labeling process that may interfere with...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
New York :
Novinka
[2014]
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| Colección: | Nanotechnology science and technology series.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- ""SURFACE PLASMON RESONANCE""; ""SURFACE PLASMON RESONANCE""; ""Library of Congress Cataloging-in-Publication Data""; ""CONTENTS""; ""PREFACE""; ""Chapter 1: INTRODUCTION""; ""OVERVIEW OF SURFACE PLASMON RESONANCE (SPR)""; ""1.1. PHYSICAL BASIS OF SURFACE PLASMON RESONANCE""; ""1.2. STEP BY STEP WORKING OF SPR""; ""Chapter 2: PRINCIPLES AND MECHANISM BEHIND SPR""; ""2.1. PHYSICAL BASIS OF SPR""; ""2.2. LOCALIZED SURFACE PLASMON RESONANCE (LSPR)""; ""2.3. SPR EMISSION""; ""Chapter 3: INSTRUMENTS BASED ON SPR PHENOMENA""; ""3.1. HISTORICAL OVERVIEW""
- ""3.2. PRINCIPLES AND MECHANISM BEHIND WORKING OF SPR BASED INSTRUMENTS""""Chapter 4: APPLICATIONS""; ""4.1. OPTICAL SENSOR BASED ON SPR OPERATING IN THE MID-INFRARED RANGE""; ""4.2. ADVANTAGES OF SPR""; ""4.3. BINDING KINETICS OF A MODEL ANTIBODY-ANTIGENSYSTEM, HSA BINDING TO ANTI-HSA IGG.ANTI-HSA IS BIOTINYLATED WITH APPROXIMATELY6 BIOTIN GROUPS AND CAPTURED ONA PLANAR NEUTR-AVIDIN SENSOR SLIDE""; ""4.4. SPR BINDING EXPERIMENT BETWEEN CAII ANDAN INHIBITOR, 4-CRBOXYBENZENESULFONAMIDE(4-CBS); A SMALL MOLECULE WITH A MOLECULARWEIGHT OF 201 DA""
- ""4.5. THERMODYNAMIC INVESTIGATION OFAN ENZYME-INHIBITOR PAIR""""4.6. SMALL VOLUME INJECTIONS WITH SR7500SYRINGE PUMP""; ""4.7. USING COMBINED ELECTROCHEMISTRY AND SPR TOMONITOR THE ELECTRO-POLYMERIZATION OF ANILINE""; ""4.8. ANISOTROPIC SURFACE PLASMON RESONANCEIMAGING BIOSENSOR""; ""4.9. ELECTROSTATIC / ELECTROCHEMICAL SPR""; ""4.10. SPR FOR DETECTING SINGLE NUCLEOTIDEPOLYMORPHISM (SNP)""; ""4.11. LOCALISED SURFACE PLASMONRESONANCE (LSPR)""; ""4.12. MAGNETIC PLASMON RESONANCE""; ""4.13. EQUILIBRIUM MEASUREMENTS(AFFINITY AND ENTHALPY)""; ""4.14. KINETIC MEASUREMENTS""
- ""4.15. ANALYSIS OF MUTANT PROTEINS""""4.16. LIMITATIONS OF SPR""; ""Chapter 5: DATA INTERPRETATION""; ""5.1. FRESNEL FORMULA""; ""5.2. BINDING CONSTANT DETERMINATION""; ""Chapter 6: GENERAL PRINCIPLES OF SPR EXPERIMENTS""; ""6.1. A TYPICAL EXPERIMENT""; ""6.2. PREPARATION OF MATERIALS AND BUFFERS""; ""6.3. MONITORING THE DIPS""; ""Chapter 7: LIGAND""; ""7.1. DIRECT VERSUS INDIRECT IMMOBILIZATION""; ""7.2. COVALENT IMMOBILISATION""; ""7.3. NON-COVALENT IMMOBILISATION (LIGAND CAPTURE)""; ""7.4. USING AN EXISTING STRATEGY""; ""7.5. DEVELOPING A NEW STRATEGY""
- ""7.6. ACTIVITY OF IMMOBILISED LIGAND""""7.7. CONTROL SURFACES""; ""7.8. RE-USING SENSOR CHIPS""; ""Chapter 8: ANALYTE""; ""8.1. PURITY, ACTIVITY AND CONCENTRATION""; ""8.2. VALENCY""; ""8.3. REFRACTIVE INDEX EFFECT AND CONTROL ANALYTES""; ""8.4. LOW MOLECULAR WEIGHT ANALYTES""; ""Chapter 9: QUALITITATIVE ANALYSIS; DO THEY INTERACT?""; ""9.1. POSITIVE AND NEGATIVE CONTROLS""; ""9.2. QUALITATIVE COMPARISONS USING A MULTIVALENT ANALYTE""; ""9.3. QUANTITATIVE MEASUREMENTS""; ""Chapter 10: AFFINITY""; ""10.1. CONCEPTS""; ""10.2. EXPERIMENTAL DESIGN""; ""10.3. PRELIMINARY STEPS""