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Genetic engineering : principles and advancements /
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Oakville, ON :
Delve Publishing,
2019.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Cover; Half Title Page; Title Page; Copyright Page; Declaration; About the Editor; Table of Contents; List of Contributors; List of Abbreviations; Preface; SECTION I GENETIC ENGINEERING IN ANIMALS; Chapter 1 Birth of Clones of The World's First Cloned Dog; Abstract; Introduction; Results and Discussion; Conclusion; Methods; References; Chapter 2 Generation of Cloned Mice and Nuclear Transfer Embryonic Stem Cell Lines From Urine-Derived Cells; Introduction; Results; Discussion; Methods; Acknowledgements; References
- Chapter 3 A One-Step Cloning Method For The Construction of Somatic Cell Gene Targeting Vectors: Application to Production of Human Knockout Cell LinesAbstract; Background; Conclusions; Methods; Acknowledgements; Authors' Contributions; References; Chapter 4 Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination; Abstract; Importance; Introduction; Results; Discussion; Materials and Methods; Acknowledgments; References; SECTION II GENETIC ENGINEERING IN PLANTS
- Chapter 5 A Modified Gateway Cloning Strategy for Overexpressing Tagged Proteins in PlantsAbstract; Background; Results; Discussion; Conclusion; Methods; Acknowledgements; Authors' Contributions; References; Chapter 6 A Simple, Flexible and Efficient PCR-Fusion/Gateway Cloning Procedure For Gene Fusion, Site-Directed Mutagenesis, Short Sequence Insertion and Domain Deletions and Swaps; Abstract; Background; Methods; Results and Discussion; Conclusion; Acknowledgements; Authors' Contributions; References
- Chapter 7 Colorful-Circuit: A Platform For Rapid Multigene Assembly, Delivery, And Expression In PlantsIntroduction; Materials And Methods; Results And Discussion; Author Contributions; Acknowledgments; References; Chapter 8 CRISPR-Based Tools For Plant Genome Engineering; Abstract; Introduction; Conclusions; Summary; References; SECTION III GENETIC ENGINEERING IN DROSOPHILA; Chapter 9 Single Vector Non-Leaky Gene Expression System For Drosophila Melanogaster; Abstract; Introduction; Results; Discussion; Methods; Acknowledgements; References
- Chapter 10 Systematic Comparison of 2A Peptides For Cloning Multi-Genes in a Polycistronic VectorAbstract; Introduction; Results And Discussion; Methods; Acknowledgements; References; Chapter 11 An Entry/Gateway® Cloning System For General Expression of Genes With Molecular Tags In Drosophila Melanogaster; Abstract; Background; Results; Discussion; Conclusion; Methods; Acknowledgements; Authors' Contributions; References; Chapter 12 Gene Tagging Strategies To Assess Protein Expression, Localization, And Function In Drosophila; Abstract; Gene Tagging Via Transgenes; Tagging Endogenous Loci In Vivo.