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Evaluation of Cellular Processes by in Vitro Assays /

Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: Gul, Taseen
Otros Autores: Balkhi, Henah Mehraj, Haq, Ehtishamul
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Sharjah : Bentham Science Publishers, 2018.
Temas:
Acceso en línea:Texto completo

MARC

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245 1 0 |a Evaluation of Cellular Processes by in Vitro Assays /  |c Taseen Gul, Henah Mehraj Balkhi & Ehtishamul Haq. 
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505 0 |a Intro; CONTENTS; FOREWORD; PREFACE; ABBREVIATIONS; ABBREVIATIONS; Basics of In Vitro Cell Culture; INTRODUCTION; 1.1. Tissue Culture and Its Types; 1.2. Cell Line; 1.3. Types of Culture; 1.4. Equipments for Cell Culture Lab; 1.5. Materials for Cell Culture; 1.6. Cryopreservation of Cell Lines; 1.7. Cell Line Authentication; 1.8. Contamination in Cell Lines; CONCLUDING REMARKS; CONSENT FOR PUBLICATION; CONFLICT OF INTEREST; ACKNOWLEDGEMENTS; REFERENCES; Methods of Transfection; INTRODUCTION; 2.1. Chemical Methods of Transfection; 2.2. Physical Methods of Gene Delivery. 
505 8 |a 2.3. Biological Methods of TransfectionCONCLUDING REMARKS; CONSENT FOR PUBLICATION; CONFLICT OF INTEREST; ACKNOWLEDGEMENTS; REFERENCES; Apoptosis; INTRODUCTION; 3.1. Mediators of Apoptosis; 3.2. Activation of Apoptosis; 3.3. Methods for Detecting Apoptosis; 3.3.1. Analysing Cytomorphological Alterations; 3.3.2. DNA Fragmentation for Detecting Apoptosis; 3.3.3. Mitochondrial Markers for Detecting Apoptosis; 3.3.4. Measuring Changes in Cell Membrane to Detect Apoptosis; 3.3.5. Measuring Caspase Activity for the Detection of Apoptosis; CONCLUDING REMARKS; CONSENT FOR PUBLICATION. 
505 8 |a CONFLICT OF INTERESTACKNOWLEDGEMENTS; REFERENCES; Cell Cytotoxicity, Viability and Proliferation; INTRODUCTION; 4.1. Trypan Blue Assay; 4.2. Neutral Red (3-Amino-7-Dimethyamino-2-Methylphenazine Hydrochlo- ride); 4.3. Calcein AM; 4.4. CFSE; 4.5. Sulphorhodamine B Assay; 4.6. Assays Based on Metabolic Activity; 4.7. Lactate Dehydrogenase Assay; 4.8. ATP Bioluminescent Assays; 4.9. Clonogenic Assay; 4.10. DNA Synthesis as a Marker for Cell Proliferation; CONCLUDING REMARKS; CONSENT FOR PUBLICATION; CONFLICT OF INTEREST; ACKNOWLEDGEMENTS; REFERENCES; Reactive Oxygen Species (ROS); INTRODUCTION. 
505 8 |a 5.1. Detection of Lipid Peroxidation5.2. Glutathione Assay; 5.3. Superoxide Assay; 5.4. Nitric Oxide Detection Assay; 5.5. Hydrogen Peroxide Detection Assay; CONCLUDING REMARKS; CONSENT FOR PUBLICATION; CONFLICT OF INTEREST; ACKNOWLEDGEMENTS; REFERENCES; Protein-Protein Interactions; INTRODUCTION; 6.1. Co-Immunoprecipitation; 6.2. Pull Down Assays; 6.3. Far-Western Blotting; CONCLUSION; CONSENT FOR PUBLICATION; CONFLICT OF INTEREST; ACKNOWLEDGEMENTS; REFERENCES; Protein-Nucleic Acid Interactions; INTRODUCTION; 7.1. Electrophoretic Mobility Shift Assay (EMSA). 
505 8 |a 7.2. Chromatin Immunoprecipitation (ChIP) Assay7.3. Affinities Capture Methods; 7.4. Pull-Down Methods; CONCLUDING REMARKS; CONSENT FOR PUBLICATION; CONFLICT OF INTEREST; ACKNOWLEDGEMENTS; REFERENCES; GLOSSARY; SUBJECT INDEX. 
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700 1 |a Balkhi, Henah Mehraj. 
700 1 |a Haq, Ehtishamul. 
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