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Microarray analysis : biochips and eradication of all diseases /
Microarray Analysis is a natural outgrowth of the author's teaching and research into the field of microarray processing, a fast growing and developing field. The book highlights the development of various microarray analysis tools and the applications of the technology towards disease preventi...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
New York [New York] (222 East 46th Street, New York, NY 10017) :
Momentum Press,
2015.
|
| Colección: | Biomedical engineering collection.
|
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- 1. Genominomics and prospects
- Chapter objectives
- Human genome project completed
- Biochips
- Next-generation sequencing
- Applications of biochips
- Consanguinous marriages
- Ashkenazi Jews
- Cystic fibrosis
- Tuberculosis
- Cancer
- Sickle-cell anemia
- Small pox
- Paleo genomes
- Colony collapse disorder
- Schizophrenia
- Prenatal testing
- DNA for forensic investigation
- Genomic surveillance
- Bio-corrosion
- Human immunodeficiency syndrome
- Microarray analysis
- Pioneers in double-helix structure of DNA
- Genominomics, economics of sequencing, $1,000
- Genome is within reach
- Genetically modified crops
- Glossary
- Summary.
- 2. Microarray analysis
- Chapter objectives
- Molecular basis for disease
- Microarray technology development
- Specifications
- Nanoprint microarrayer
- Ten tips for five-step process
- Step 1 formulation of a biologic question
- Step 2 sample preparation
- Step 3 biochemical reaction
- Step 4 microarray detection
- Step 5 microarray data analysis and modeling
- Microarray fluorescence detection
- Confocal scanning microscope
- Quality of substrate surface
- Phosphoramadite synthesis
- Summary.
- 3. Sequencing technology advances
- Chapter objectives
- Genomes completed
- Next-generation sequencers
- Gene mapping
- Electrophoretic methods
- Microfluidic separations
- Polymer liquids
- Transport parameters
- Transient concentration profile predicted using the a capite ad calcem concentration non-Fick diffusion equation in a semi-infinite medium
- Convection and diffusion
- Summary.
- 4. Applications
- Chapter objectives
- Cancer
- Kinetic model for progression of cancer
- Proteomics and Michaelis and Menten kinetics
- Immune action mechanism
- DNA hybridization kinetics-diffusion effects
- Gene modifications
- DNA-melting temperature
- Genetic disorders and microarray analysis
- Tissue microarrays and cell microarrays
- Bio-based polymers
- Gene silencing
- Metabolomics
- Recombinant DNA technology and genetically
- Modified crops
- Biodiesel
- Consecutive-competitive reactions
- Centrifugal separation of fame and glycerol: torque requirements
- Shear flow theory
- Results
- Gene therapy
- Gene activity in songbirds similar to humans
- Glossary
- Summary.
- 5. Next-generation sequencing
- Chapter objectives
- Blotting techniques
- Sanger sequencing
- Sequencing by synthesis
- Sequencing by ligation
- Pyrosequencing
- Single-molecule sequencing
- DNA sequencing through nanopore
- Glossary
- Summary.
- 6. Biochip manufacturing
- Chapter objectives
- Three approaches
- Ex situ manufacturing
- Commercial instruments
- Time to print
- Summary.
- 7. Statistical characterization and normalization
- Chapter objectives
- Housekeeping genes and normalization
- Clustering
- Supervised
- Variation filter
- Unsupervised
- Pearson's correlation coefficient
- Principal component analysis
- Cluster determination
- Nearest neighbor clustering
- Unsupervised classification
- Silhouette method
- Dunn's validation index
- Davies-Bouldin index
- C-index
- Self-organizing maps
- K-means clustering
- Agglomerative clustering
- Dendrograms
- Two-dimensional dendrograms
- Division or partition clustering
- Bayesian clustering
- Boolean networks
- Bayesian networks
- Relevance networks
- Glossary
- Summary.