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Metagenomics : methods, applications and perspectives /
Metagenomic analysis has extraordinary potential to improve our understanding of microbial populations in their natural environment and identify novel genes of interest. The key feature of such analyses is that they are performed using metagenomic libraries constructed from total DNA isolated from a...
| Clasificación: | Libro Electrónico |
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| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
New York :
Nova Publishers,
[2014]
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| Colección: | Microbiology research advances series.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- METAGENOMICS: METHODS, APPLICATIONS AND PERSPECTIVES; METAGENOMICS: METHODS, APPLICATIONS AND PERSPECTIVES; Library of Congress Cataloging-in-Publication Data; CONTENTS; PREFACE; Chapter 1: POTENTIAL AND LIMITATIONS OF METAGENOMIC FUNCTIONAL ANALYSES; ABSTRACT; 1. MICROBIAL DIVERSITY; 2. OVERVIEW OF METAGENOMIC LIBRARY CONSTRUCTION; 3. METAGENOMIC LIBRARY ANALYSES; 4. FUNCTIONAL SCREENS; 5. METAGENOMIC GENE EXPRESION; 6. FUTURE PROSPECTS FOR IDENTIFICATION OF METAGENOMIC FUNCTIONS; REFERENCES; Chapter 2: METAGENOMICS: LIBRARY CONSTRUCTION AND SCREENING METHODS; ABSTRACT; 1. INTRODUCTION.
- 2. EXTRACTION OF NUCLEIC ACIDS FROM ENVIRONMENTAL SAMPLES3. ENRICHMENT OF ENVIRONMENTAL SAMPLES; 4. CONSTRUCTION OF A METAGENOMIC LIBRARY; 5. SCREENING OF METAGENOMIC DATA; CONCLUSION; REFERENCES; Chapter 3: THE USE OF ION TORRENT PGM FOR BACTERIAL DIVERSITY ANALYSES: THE STUDY CASE OF FIVE BRAZILIAN HYDROELECTRIC RESERVOIRS; ABSTRACT; INTRODUCTION; CONCLUSION; REFERENCES; Chapter 4: METAGENOMIC PROFILING FOR ASSESSING ENVIRONMENTAL HEALTHY; ABSTRACT; 1. INTRODUCTION; 2. EVALUATING ANTHROPOGENIC IMPACTS THROUGH METAGENOMICS; 3. APPLICATIONS OF METAGENOMICS TO PUBLIC HEALTH; REFERENCES.
- Chapter 5: MINING NOVEL GENES AND ENZYMES OF UNCULTURED EUKARYOTIC MICROORGANISMS BY AN RNA-BASED APPROACHABSTRACT; METAGENOMICS: ACCESS TO THE UNCULTURED MICROBIAL RESOURCES; TARGET OF METAGENOMIC ANALYSIS; APPROACHES TO METAGENOMIC SCREENING; EUKARYOTIC CELLS AS A TARGET RESOURCE FOR METAGENOMIC ANALYSIS; EXPLORING THE FUNCTIONAL EUKARYOTIC GENES BY A METATRANSCRIPTOMIC APPROACH; CONCLUDING REMARKS AND FUTURE PROSPECTS; REFERENCES; Chapter 6: DEVELOPMENTS IN THE RETRIEVAL OF NOVEL BIOCATALYSTS BY METAGENOMIC APPROACHES; ABSTRACT; INTRODUCTION.
- OVERCOMING THE PROBLEMS IN THE EXTRACTION AND OBTAINING HUMUS-FREE PURE ENVIRONMENTAL DNAPROBLEMS IN SCREENING METAGENOMIC LIBRARIES; PROGRESS ACHIEVED IN DISCOVERING A VARIETY OF BIOCATALYSTS; OTHER INDUSTRIALLY IMPORTANT ENZYMES; SUCCESS STORIES AND COMMERCIALIZED METAGENOMIC BIOCATALYSTS; CONCLUSION; REFERENCES; Chapter 7: ISOLATION OF SOIL METAGENOMIC DNA: CHALLENGES AND SOLUTIONS; ABSTRACT; INTRODUCTION; 1. ORIGIN OF METAGENOMICS; 2. SOIL
- THE ECOSYSTEM OF CHOICE FOR METAGENOMIC STUDIES; 3. ISOLATION OF METAGENOMIC DNA; CONCLUSION; REFERENCES.
- Chapter 8: MICROBIAL EXPLORATION IN EXTREMECONDITIONS: METAGENOMIC ANALYSISAND FUTURE PERSPECTIVESABSTRACT; INTRODUCTION; LIMITATIONS OF CONVENTIONAL MICROBIAL METHODS; SANGER AND NEXT GENERATION SEQUENCING; THE METAGENOMICS PIPELINE; BIOINFORMATICS AND THERMOPHILE METAGENOMICS; ANNOTATION OF METAGENOMIC SEQUENCES; DIFFERENT METAGENOMIC APPROACHES FOR MICROBIOLOGY; SEQUENCE-DRIVEN METAGENOMICS; FUNCTION-DRIVEN METAGENOMICS; EXTREME ECOLOGICAL ENVIRONMENTS; HOT WATER SPRINGS; THERMOPHILIC MICROORGANISMS IN HOT SPRINGS; THERMOPHILIC HOT SPRINGS BACTERIA; THERMOPHILIC VIRUSES.