Synthetic biology : a lab manual /

Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autores principales: Liljeruhm, Josefine (Autor), Gullberg, Erik (Autor), Forster, Anthony C. (Autor)
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Hackensack, NJ : World Scientific Publishing Company, 2014.
Temas:
Synthetic biology > Laboratory manuals.
Biologie synthétique > Manuels de laboratoire.
SCIENCE > Chemistry > Industrial & Technical.
TECHNOLOGY & ENGINEERING > Chemical & Biochemical.
Synthetic biology
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • 1. Introduction. What is synthetic biology, exactly? The iGEM outbreak. A synthetic biology lab manual
  • 2. Genes, chromoproteins and antisense RNAs. E. coli DNA: Chromosomes, plasmids and copy number. Coupling of transcription and translation in bacteria. Promoter and terminator for transcription. Ribosome binding site (RBS). Codon bias. Chromoproteins. Small regulatory RNAs (sRNAs)
  • 3. Lab rooms and equipment. The physical lab spaces. Equipment
  • 4. Safety is priority #1. Fires. Chemicals. Biological safety and disposal. Dangerous equipment
  • 5. Lab course projects. Time and resources. Project overview and learning objectives. The lab notebook. Lab section 1. Preparation of chemical solutions and agar plates. Lab section 2. Coloring bacteria by adding a promoter to a chromoprotein gene. Lab section 3: Rational engineering of chromoprotein expression level. Lab section 4. Other experiments. The "dreaded" exam
  • 6. Protocols. Introduction. Protocol 1. Preparation of solutions and agar plates. Protocol 2. Overnight cultures with antibiotics, and glycerol stocks. Protocol 3. BioBrick 3A assembly and gel analysis. Protocol 4. Agarose gel electrophoresis. Protocol 5. Preparation of competent E. coli cells using CaCl2. Protocol 6. Transformation of CaCl2-competent E. coli cells. Protocol 7. Bacterial re-streaking techniques. Protocol 8. Lysis of E. coli cells with lysozyme. Protocol 9. Polymerase chain reaction (PCR). Protocol 10. Inverse PCR mutagenesis. Protocol 11. Colony PCR. Protocol 12. Gibson assembly
  • 7. Advanced methods. Flow cytometry and cell sorting. Recombination in plasmids and the chromosome. Electrocompetent cells
  • 8. The International Genetically Engineered Machine (iGEM) Competition. How to start an iGEM team. Uppsala iGEM 2011
  • Show color with color. Uppsala iGEM 2012
  • Resistance is futile. Uppsala iGEM 2013
  • Lactonutritious
  • it's delicious
  • 9. Appendices.

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