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Biophysical techniques /
Many current questions in biology are probed by using a range of biophysical methods, often in tandem. These increasingly powerful and sophisticated tools allow us to study the structure and dynamics of the complicated mixture of interacting molecules that make up the living cell. Biophysical Techni...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Oxford :
Oxford University Press,
[2012]
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Cover; Contents; Some frequently used abbreviations; 1 Introduction; What are "biophysical techniques"?; What questions can biophysical techniques answer?; Which technique to use?; Organization of this book; 2 Molecular principles; 2.1 Molecules and interactions; Introduction; Box 2.1 Atoms and elements; Box 2.2 Kinetic model of gases; Non-covalent interactions; Box 2.3 Electrostatics, dielectrics, and polarity; Box 2.4 Molecular biology tools and base pairing; Binding; 3 Transport and heat; 3.1 Diffusion, osmosis, viscosity, and friction; Introduction; Diffusion; Osmosis.
- Application of a force to a molecule in solutionViscosity; Box 3.1 The frictional coeffi cient, f, depends on molecular shape; 3.2 Analytical centrifugation; Introduction; Some basic principles of sedimentation; Sedimentation velocity; Sedimentation equilibrium; Density gradient sedimentation; 3.3 Chromatography; Introduction; Theory; Chromatography techniques; Quantitative chromatography; 3.4 Electrophoresis; Introduction; Theory; Experimental; Some electrophoresis systems; 3.5 Mass spectrometry; Introduction; Ionization; Ion sorting/analysis; Applications of mass spectrometry.
- 3.6 ElectrophysiologyIntroduction; Membrane potential; Action potentials; Experimental; Propagation of an action potential in a neuron; 3.7 Calorimetry; Introduction; Isothermal titration calorimetry; Differential scanning calorimetry; Box 3.2 Heat capacity; 4 Scattering, refraction, and diffraction; 4.1 Scattering of radiation; Introduction; Scattering theory; Box 4.1 Weight average molecular weight; Turbidity; Solution scattering and molecular shape; Box 4.2 Radii of gyration, R[sub(G)], and hydration, R[sub(H)]; Dynamic light scattering; Box 4.3 Correlation functions.
- 4.2 Refraction, evanescent waves, and plasmonsIntroduction; Box 4.4 Classical optics; Evanescent waves; Surface plasmon resonance; Box 4.5 The streptavidin/biotin complex; 4.3 Diffraction; Introduction; Principles of diff raction; Box 4.6 Single particle imaging with X-ray laser; Diffraction experiments; Interpretation of diffraction data; Other crystallography techniques; Achievements of crystallography; 5 Electronic and vibrational spectroscopy; 5.1 Introduction to absorption and emission spectra; Introduction; Energy states; Absorption.
- Box 5.1 Transition dipole moments and transition probabilityEmission; Box 5.2 The laser; 5.2 Infrared and Raman spectroscopy; Introduction; IR spectra and applications; Raman scattering; Applications of Raman spectroscopy; 5.3 Ultraviolet/visible spectroscopy; Introduction; Measurement of electronic spectra; Electronic energy levels and transitions; Absorption properties of some key chromophores; Applications of UV/visible spectra; Box 5.3 Isosbestic points; Properties associated with the direction of the transition dipole moment; Monitoring rapid reactions; 5.4 Optical activity; Introduction.