Cargando…
Biophysical techniques /
Many current questions in biology are probed by using a range of biophysical methods, often in tandem. These increasingly powerful and sophisticated tools allow us to study the structure and dynamics of the complicated mixture of interacting molecules that make up the living cell. Biophysical Techni...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Oxford :
Oxford University Press,
[2012]
|
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
| LEADER | 00000cam a2200000 i 4500 | ||
|---|---|---|---|
| 001 | EBSCO_ocn867050461 | ||
| 003 | OCoLC | ||
| 005 | 20231017213018.0 | ||
| 006 | m o d | ||
| 007 | cr mn||||||||| | ||
| 008 | 140102t20122012enka ob 001 0 eng d | ||
| 040 | |a N$T |b eng |e rda |e pn |c N$T |d IDEBK |d OCLCO |d OCLCF |d YDXCP |d EBLCP |d DEBSZ |d OCLCO |d OCLCQ |d OCLCO |d OCLCQ |d OCLCO |d OCLCA |d YDX |d OSU |d OCLCO |d CRU |d OCLCO |d AGLDB |d OCLCO |d OCLCQ |d IGB |d OCLCO |d OCLCA |d D6H |d OCLCO |d VNS |d OCLCO |d OCLCQ |d VTS |d OCLCO |d OCLCQ |d OCLCO |d STF |d S2H |d OCLCA |d VLY |d UKAHL |d OCLCO |d OCLCQ | ||
| 019 | |a 866840770 |a 867929645 |a 1117105139 |a 1162056371 |a 1241857127 |a 1300506176 | ||
| 020 | |a 9780191666148 |q (electronic bk.) | ||
| 020 | |a 0191666149 |q (electronic bk.) | ||
| 020 | |a 9781306263337 |q (ebk) | ||
| 020 | |a 1306263336 |q (ebk) | ||
| 020 | |z 9780199642144 |q (pbk.) | ||
| 020 | |z 0199642141 |q (pbk.) | ||
| 029 | 1 | |a DEBSZ |b 399652019 | |
| 029 | 1 | |a DEBSZ |b 442983700 | |
| 029 | 1 | |a DEBSZ |b 493135871 | |
| 035 | |a (OCoLC)867050461 |z (OCoLC)866840770 |z (OCoLC)867929645 |z (OCoLC)1117105139 |z (OCoLC)1162056371 |z (OCoLC)1241857127 |z (OCoLC)1300506176 | ||
| 037 | |a 557584 |b MIL | ||
| 050 | 4 | |a QH505 |b .C35 2012eb | |
| 060 | 4 | |a 2013 E-452 | |
| 060 | 4 | |a QU 34 | |
| 072 | 7 | |a SCI |x 056000 |2 bisacsh | |
| 082 | 0 | 4 | |a 571.4 |2 23 |
| 084 | |a WC 2050 |2 rvk | ||
| 084 | |a CHE 802f |2 stub | ||
| 049 | |a UAMI | ||
| 100 | 1 | |a Campbell, Iain D., |e author. | |
| 245 | 1 | 0 | |a Biophysical techniques / |c Iain D. Campbell. |
| 264 | 1 | |a Oxford : |b Oxford University Press, |c [2012] | |
| 264 | 4 | |c ©2012 | |
| 300 | |a 1 online resource (ix, 353 pages) : |b illustrations (some color) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 520 | |a Many current questions in biology are probed by using a range of biophysical methods, often in tandem. These increasingly powerful and sophisticated tools allow us to study the structure and dynamics of the complicated mixture of interacting molecules that make up the living cell. Biophysical Techniques explains in a readily-accessible way the basics of the various methods available - including those used to study molecular structure, cell structure, and dynamic interactions - so that students can understand the principles behind the different methods used, and begin to appreciate which tools can be used to probe different biological questions, and the pros and cons of each. Exploring the latest enhancements to classical techniques, and introducing computational techniques that have emerged relatively recently, the book provides a broad survey of the range of techniques now at the disposal of an investigator. The biophysical techniques introduced can be explained in physical and mathematical terms; instead of assuming a detailed knowledge of physics or maths, however, the book uses a series of Tutorials and Boxes to provide essential background guidance on these topics, giving the reader extra support in fully understanding the subject. Learning and understanding is supported still further with thought-provoking end-of-chapter problems, and a range of online Journal Clubs, to illustrate to the reader how a range of biophysical techniques have been used in contemporary research. In an age in which we are probing the working of biological systems in more detail, and with more refinement, than ever before, Biophysical Techniques is the ideal guide for any student who needs to understand the power of biophysical techniques in biological research. Online Resource Centre For students: Journal Clubs for a number of chapters help the reader to explore how biophysical techniques are used in active research For registered adopters of the text: Figures from the book in electronic format | ||
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | |a Cover; Contents; Some frequently used abbreviations; 1 Introduction; What are "biophysical techniques"?; What questions can biophysical techniques answer?; Which technique to use?; Organization of this book; 2 Molecular principles; 2.1 Molecules and interactions; Introduction; Box 2.1 Atoms and elements; Box 2.2 Kinetic model of gases; Non-covalent interactions; Box 2.3 Electrostatics, dielectrics, and polarity; Box 2.4 Molecular biology tools and base pairing; Binding; 3 Transport and heat; 3.1 Diffusion, osmosis, viscosity, and friction; Introduction; Diffusion; Osmosis. | |
| 505 | 8 | |a Application of a force to a molecule in solutionViscosity; Box 3.1 The frictional coeffi cient, f, depends on molecular shape; 3.2 Analytical centrifugation; Introduction; Some basic principles of sedimentation; Sedimentation velocity; Sedimentation equilibrium; Density gradient sedimentation; 3.3 Chromatography; Introduction; Theory; Chromatography techniques; Quantitative chromatography; 3.4 Electrophoresis; Introduction; Theory; Experimental; Some electrophoresis systems; 3.5 Mass spectrometry; Introduction; Ionization; Ion sorting/analysis; Applications of mass spectrometry. | |
| 505 | 8 | |a 3.6 ElectrophysiologyIntroduction; Membrane potential; Action potentials; Experimental; Propagation of an action potential in a neuron; 3.7 Calorimetry; Introduction; Isothermal titration calorimetry; Differential scanning calorimetry; Box 3.2 Heat capacity; 4 Scattering, refraction, and diffraction; 4.1 Scattering of radiation; Introduction; Scattering theory; Box 4.1 Weight average molecular weight; Turbidity; Solution scattering and molecular shape; Box 4.2 Radii of gyration, R[sub(G)], and hydration, R[sub(H)]; Dynamic light scattering; Box 4.3 Correlation functions. | |
| 505 | 8 | |a 4.2 Refraction, evanescent waves, and plasmonsIntroduction; Box 4.4 Classical optics; Evanescent waves; Surface plasmon resonance; Box 4.5 The streptavidin/biotin complex; 4.3 Diffraction; Introduction; Principles of diff raction; Box 4.6 Single particle imaging with X-ray laser; Diffraction experiments; Interpretation of diffraction data; Other crystallography techniques; Achievements of crystallography; 5 Electronic and vibrational spectroscopy; 5.1 Introduction to absorption and emission spectra; Introduction; Energy states; Absorption. | |
| 505 | 8 | |a Box 5.1 Transition dipole moments and transition probabilityEmission; Box 5.2 The laser; 5.2 Infrared and Raman spectroscopy; Introduction; IR spectra and applications; Raman scattering; Applications of Raman spectroscopy; 5.3 Ultraviolet/visible spectroscopy; Introduction; Measurement of electronic spectra; Electronic energy levels and transitions; Absorption properties of some key chromophores; Applications of UV/visible spectra; Box 5.3 Isosbestic points; Properties associated with the direction of the transition dipole moment; Monitoring rapid reactions; 5.4 Optical activity; Introduction. | |
| 588 | 0 | |a Print version record. | |
| 546 | |a English. | ||
| 590 | |a eBooks on EBSCOhost |b EBSCO eBook Subscription Academic Collection - Worldwide | ||
| 650 | 0 | |a Biophysics |v Textbooks. | |
| 650 | 0 | |a Biological systems |x Mathematical models. | |
| 650 | 0 | |a Molecular biology |x Technique. | |
| 650 | 2 | 2 | |a Molecular Diagnostic Techniques |x methods |
| 650 | 6 | |a Systèmes biologiques |x Modèles mathématiques. | |
| 650 | 6 | |a Biologie moléculaire |x Technique. | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Anatomy & Physiology. |2 bisacsh | |
| 650 | 7 | |a Biological systems |x Mathematical models. |2 fast |0 (OCoLC)fst00832340 | |
| 650 | 7 | |a Biophysics. |2 fast |0 (OCoLC)fst00832656 | |
| 650 | 7 | |a Molecular biology |x Technique. |2 fast |0 (OCoLC)fst01024758 | |
| 650 | 7 | |a Biophysik |2 gnd | |
| 650 | 7 | |a Methode |2 gnd | |
| 655 | 7 | |a Textbooks. |2 fast |0 (OCoLC)fst01423863 | |
| 776 | 0 | 8 | |i Print version: |a Campbell, Iain D. |t Biophysical techniques. |d Oxford : Oxford University Press, ©2012 |z 9780199642144 |w (DLC) 2011944050 |w (OCoLC)757930885 |
| 856 | 4 | 0 | |u https://ebsco.uam.elogim.com/login.aspx?direct=true&scope=site&db=nlebk&AN=678131 |z Texto completo |
| 938 | |a Askews and Holts Library Services |b ASKH |n AH25955221 | ||
| 938 | |a EBSCOhost |b EBSC |n 678131 | ||
| 938 | |a ProQuest MyiLibrary Digital eBook Collection |b IDEB |n cis27167092 | ||
| 938 | |a YBP Library Services |b YANK |n 11441727 | ||
| 994 | |a 92 |b IZTAP | ||