Cargando…
Single-molecule cellular biophysics /
"Recent advances in single molecule science have presented a new branch of science: single molecule cellular biophysics, combining classical cell biology with cutting-edge single molecule biophysics. This textbook explains the essential elements of this new discipline, from the state-of-the-art...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Cambridge :
Cambridge University Press,
2013.
|
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Preface; Life, from the bottom up; References; 1 Once upon a (length and) time (scale); 1.1 Introduction; 1.2 There are already many informative `multi-molecule ́ methods; 1.2.1 Calorimetry; 1.2.2 Chromatography and dialysis; 1.2.3 Circular dichroism and optical rotation; 1.2.4 Electron microscopy; 1.2.5 Electrophysiology; 1.2.6 Fluorimetry; 1.2.7 Gel eletrophoresis; 1.2.8 Mass spectrometry; 1.2.9 NMR and ESR spectroscopy; 1.2.10 Optical interferometry; 1.2.11 Optical microscopy; 1.2.12 Radioactivity; 1.2.13 Spectrophotometry; 1.2.14 Sedimentation methods.
- 1.2.15 X-ray, neutron and electron diffraction1.3 American versus European coffee; 1.4 Scales of length, force, energy, time and concentration; 1.4.1 Length; 1.4.2 Force; 1.4.3 Energy; 1.4.4 Time; 1.4.5 Concentration; 1.5 Some basic thermodynamics of life; 1.6 The concept of 'functionality'; 1.7 Test tube or cell?; References; General; Advanced; Questions; For the life scientists; For the physical scientists; For those who have not made up their mind; 2 The molecules of life
- an idiot's guide; 2.1 Introduction; 2.2 The atomic components of single biological molecules.
- 2.3 Cell structure and sub-cellular architecture2.4 Amino acids, peptides and proteins; 2.5 Sugars; 2.6 Nucleic acids; 2.7 Lipids; 2.8 Miscellaneous 'small' molecules; 2.9 The 'central dogma' of molecular biology; 2.10 Molecular simulations; 2.11 Importance of non-covalent forces; References; General; Advanced; Questions; For the life scientists; For the physical scientists; For those who have not made up their mind; 3 Making the invisible visible: part 1
- methods that use visible light; 3.1 Introduction; 3.2 Magnifying images.
- 3.3 Generating optical contrast using scattered light or fluorescence3.4 Organic dyes, FlAsH/ReAsH, fluorescent amino acids and quantum dots; 3.5 Fluorescent proteins, SNAP/CLIP-Tags and HaloTags; 3.6 Illuminating and detecting fluorescent tags; 3.6.1 Widefield modes of epifluorescence and oblique epifluorescence; 3.6.2 Slimfield and narrow-field epifluorescence; 3.6.3 Confocal microscopy; 3.6.4 Multi-photon excitation; 3.6.5 Optical lock-in detection; 3.6.6 Light sheet microscopy
- SPIM; 3.6.7 Adaptive optics; 3.7 Fluorescence correlation spectrosopy (FCS).
- 3.8 Fluorescence lifetime imaging (FLIM)3.9 'Super-resolution' techniques; 3.9.1 Iterative fitting (FIONA-type) approaches; 3.9.2 Sub-stoichiometric labelling; 3.9.3 Total internal reflection fluorescence (TIRF); 3.9.4 Stochastic activation, switching and blinking of fluorescent dyes; 3.9.5 Shrinking the point spread function; 3.9.6 Near-field approaches; 3.9.7 Structured illumination; 3.9.8 Förster resonance energy transfer; 3.10 'Multi-dimensional' imaging; References; General; Advanced; Questions; For the life scientists; For the physical scientists.