Recent trends in gene expression /
Clasificación: | Libro Electrónico |
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Otros Autores: | |
Formato: | Electrónico eBook |
Idioma: | Inglés |
Publicado: |
New York :
Nova Biomedical,
c2013.
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Colección: | Genetics--research and issues series.
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Temas: | |
Acceso en línea: | Texto completo |
Tabla de Contenidos:
- RECENT TRENDS IN GENE EXPRESSION ; Library of Congress Cataloging-in-Publication Data ; Contents; Preface; Chapter I Mechanism of Transcription in Prokaryotes ; Introduction; RNA Polymerase ; RNAP Core ; RNAP Holoenzyme ; Closed Complex ; Open Complex ; Abortive Initiation ; Elongation; Termination; Intrinsic Termination ; Rho Dependent Termination ; References; Chapter II How Noisy Is RNA Production in Escherichia Coli When Seen in Vivo, One Event at a Time ; Abstract; Introduction; Methods ; Strains and Plasmids.
- Genetic Constructs to Observe the Dynamics of Expression of the Tet Promoter Growth Conditions of the Cells ; Inducing the Expression of the RNA Target for MS2-GFP ; Time-Lapse Fluorescence Microscopy with Auto-Focusing ; qPCR Analysis of Target RNA ; Fluorescent Microplate Reader Measurement for Mean Protein Levels ; Synchronizing Cell Divisions by Heat Shock and Optical Density Measurements; Image Processing of Live Cell Imaging Results ; Inferring the Number of Sequential Steps in Transcription Initiation.
- Modeling Populations of Cells With Stochastic Gene Expression and Probabilistic RNA Partitioning During Cell Division Kinetics of Transcript Production ; The Lar Promoter ; The Tet Promoter ; Probabilistic RNA Partitioning ; Conclusion; References; Chapter III The Biochemistry of F420 Cofactor Biosynthesis ; Abstract; Introduction; Phylogenetic Distribution of the F 420 Cofactor ; Biosynthesis of the F420 Cofactor ; Enzymes: Relevance of Cofactor through Enzymes ; F420 Reducing Hydrogenase (FRH) ; F420-Dependent N,N-Methylenetetrahydromethanopterin Dehydrogenase (MTD)
- F420-Dependent N5,N10-Methylenetetrahydromethanopterin Reductase (MER) F420-Dependent NADP+ Oxidoreductase (FNO) ; Formate Dehydrogenase (FDH) ; F420-Dependent Alcohol Dehydrogenase ; F420-Dependent Glucose-6-Phosphate Dehydrogenase (FGD) ; Conclusion; Acknowledgments; References; Chapter IV Regulation of Gene Expression through mRNA Stability: Implications in Cancer Development and Therapeutics ; Abstract; Abbreviations; Introduction; mRNA Stability and Degradation ; Trans-Acting RNA Binding Proteins ; Trans-Acting miRNAs ; mRNA Stability Involved in Cancer.
- MRNA Stability and Cancer Therapeutics Conclusion; References; Chapter V Roles of Small RNAs in Regulation of Signaling and Adaptive Responses in Plants ; Abstract; Introduction: Adaptable Phenotypes Are the Key to Survival ; smRNA Pathway in Plants ; Three Pillars of smRNA Pathways: RdRS, DCLS, and AGOS ; Approaches to Identify smRNA Targets ; Experimental Approaches to smRNA Target Identification ; smRNAs in Signaling and Defense in Plants; Conclusion and Future Perspective ; Acknowledgments; References; Chapter VI Nuclear Organization of Cancer Cells ; Abstract; Introduction.
- Cancer
- A Unique Model System to Study Nuclear Structure-Function Relationships.