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Update on Production of Recombinant Therapeutic Protein : Transient Gene Expression.
Over the past decade, the transient gene expression (TGE) technology platform has been actively pursued to produce a wide range of therapeutic proteins, monoclonal antibodies, and vaccines for mainly preclinical assessment, due to its short development times and low overall cost. This book updates t...
| Clasificación: | Libro Electrónico |
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| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Shrewsbury :
ISmithers Rapra Publishing,
2013.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Acknowledgements ; Preface ; Contributors ; 1 Transient Gene Expression in Different Expression Systems ; 1.1 Introduction ; 1.2 Transient Gene Expression versus Stable Gene Expression ; 1.3 Transient Gene Expression in Different Systems ; 1.3.1 Mammalian Cell Systems ; 1.3.2 Plant Systems ; 1.3.3 Insect Cell Systems ; 1.3.4 Stem Cell Systems ; References ; 2 Recent Advances in Transient Gene Expression Protocol ; 2.1 Vectors ; 2.1.1 Viral Vector ; 2.1.1.1 Adenovirus ; 2.1.1.2 Lentiviruses ; 2.1.1.3 Baculovirus ; 2.1.1.4 Vaccinia Virus
- 2.1.1.5 Alphavirus 2.1.2 Nonviral Vectors ; 2.2 Construction for Expression ; 2.2.1 Promoter ; 2.2.2 Other Construction Components ; 2.2.3 Plasmid Preparation and Quality ; 2.3 Nonviral Gene Delivery ; 2.3.1 Electroporation Methods ; 2.3.2 Chemical Methods ; 2.3.2.1 Calcium Phosphate ; 2.3.2.2 Cationic Lipids ; 2.3.2.3 Polythylenimine ; 2.4 Cell Lines used in Transient Gene Expression ; 2.4.1 Human Embryonic Kidney 293 Cells ; 2.4.2 Chinese Hamster Ovary Cells ; 2.4.3 Other Cell Lines ; 2.4.3.1 Hybrid of Human Kidney and B Cells-11 ; 2.4.3.2 CAP-T Cell Line
- 2.4.3.3 CAP-T versus Chinese Hamster Ovary versus Human Embryonic Kidney 293-6E and Hybrid of Huma 2.4.3.4 Huh-7 Cell Line ; 2.4.3.5 VERO Cell Line ; 2.4.3.6 PER.C6 Cell Line ; 2.5 Current Transient Gene Expression Protocols ; 2.5.1 Shake Flask Protocol for Volumes of Normal and High Density Cell Cultures Greater than One Lit; 2.5.2 Protocol for Large-scale Transient Transfection in the Wave Bioreactor [71, 110] ; 2.5.2.1 Large scale (20 L) Chinese Hamster Ovary Cell Transfection in Wave Bioreactor [110]
- 2.5.2.2 Large scale (20 L) Human Embryonic Kidney 293 Cell Transfection in Wave Bioreactor [71] 2.5.3 High Density Large-scale Transfection of Mammalian Cells [109] ; 2.5.3.1 Cell Cultivation ; 2.5.3.2 Cell Expansion for Transfection ; 2.5.4 100 L Transient Gene Expression Protocol [4] ; 2.5.5 Purification of Products from Transient Gene Expression ; 2.5.5.1 Purification of a Monoclonal Antibody from Transient Gene Expression ; 2.5.5.2 Purification of a Recombinant Protein from Transient Gene Expression ; References
- 3 Optimisation of Transient Gene Expression for Therapeutic Protein Production 3.1 Optimisation of the Transient Gene Expression Conditions ; 3.1.1 Medium Optimisation ; 3.1.1.1 Peptones ; 3.1.1.2 Valproic Acid ; 3.1.1.3 Other Additives ; 3.1.2 Optimisation of Transient Gene Expression Conditions and Procedures ; 3.1.2.1 Process Design ; 3.1.2.2 Optimisation of Culture Conditions ; 3.1.3 Construction Optimisation ; 3.1.3.1 Promoter ; 3.1.3.2 Codon Optimisation ; 3.1.3.3 Leader Sequence ; 3.1.3.4 Other Genetic Elements ; 3.1.3.4.1 Post-transcriptional Regulatory Elements