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Insect molecular genetics : an introduction to principles and applications /
This book summarizes and synthesizes two rather disparate disciplines-entomology and molecular genetics. It provides an introduction to the techniques and literature of molecular genetics; defines terminology; and reviews concepts, principles, and applications of these powerful tools.
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam :
Academic Press,
[2013]
|
| Edición: | Third edition. |
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 100 | 1 | |a Hoy, Marjorie A., |e author. | |
| 245 | 1 | 0 | |a Insect molecular genetics : |b an introduction to principles and applications / |c Marjorie A. Hoy. |
| 250 | |a Third edition. | ||
| 264 | 1 | |a Amsterdam : |b Academic Press, |c [2013] | |
| 264 | 4 | |c ©2013 | |
| 300 | |a 1 online resource (xxvii, 808 pages) : |b illustrations (some color) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 504 | |a Includes bibliographical references and index. | ||
| 588 | 0 | |a Print version record. | |
| 505 | 0 | |6 880-01 |a Note continued: 9.3.P Elements and Hybrid Dysgenesis -- 9.4.P-Element Structure Varies -- 9.5. Transposition Method of P Elements -- 9.6. Origin of P Elements in D. melanogaster -- 9.7.P Vectors and Germ-Line Transformation -- 9.7.1. Protocols -- 9.7.2. Characterizing Transformants -- 9.8. Using P-Element Vectors -- 9.8.1. Transposon Tagging -- 9.8.2. Expressing Exogenous Genes -- 9.8.3. Evaluating Position Effects -- 9.8.4. Targeted Gene Transfer -- 9.9. Transformation of Other Insects with P Vectors -- 9.10. Evolution of Resistance to P Elements -- 9.11. Using P to Drive Genes into Populations -- 9.12. Relationship of P to Other Transposable Elements (TEs) -- 9.13. Other TEs Can Transform D. melanogaster -- 9.14. Improved Transformation Tools for Drosophila -- 9.15. TE Vectors to Transform Insects other than Drosophila -- 9.15.1.piggyBac -- 9.15.2. Hermes and Herves -- 9.15.3. Minos -- 9.15.4.mariner -- 9.15.5.hobo -- 9.16. Cross Mobilization of TE Vectors -- 9.17. Conversion of Inactive TE Vectors to Activity -- 9.18. Suppression of Transgene Expression -- 9.19. Other Transformation Methods -- 9.19.1. JcDNV Gene Vectors for Somatic Transformations v -- 9.19.2. RNAi for Drosophila -- 9.19.3. Zinc-Finger Nucleases (ZFNs) -- 9.19.4. Transcription Activator-Like Effector Nucleases (TALENs) -- 9.19.5. Meganucleases (or Homing Endonucleases) -- 9.19.6. Cell-Penetrating Peptides -- 9.19.7. Nanotechnology Approaches -- 9.20. Conclusions -- General References -- References Cited -- pt. III APPLICATIONS IN ENTOMOLOGY -- ch. 10 Sex Determination in Insects -- 10.1. Overview -- 10.2. Introduction -- 10.3. Costs and Benefits of Sexual Reproduction -- 10.3.1. Sexual Reproduction Has Costs -- 10.3.2. Advantages of Sex Must Be Large -- 10.3.3. Origin of Sex -- 10.4. Sex Determination Involves Soma and Germ-Line Tissues -- 10.5. Sex Determination in Drosophila melanogaster -- 10.5.1. Dosage Compensation of X Chromosomes -- 10.5.2. Somatic-Sex Determination -- 10.5.3. Germ-Line Determination -- 10.6. Are Sex-Determination Mechanisms Diverse? -- 10.6.1. Intraspecific Variability -- 10.6.2. Environmental Effects -- 10.6.3. Postzygotic Sex Determination -- 10.7.A Single Model? -- 10.8. Meiotic Drive Can Distort Sex Ratios -- 10.8.1. Segregation Distorter (SD) -- 10.8.2. Distorter in Mosquitoes -- 10.8.3. Female-Biased Sex Ratios in Stalk-Eyed Flies -- 10.8.4. Meiotic Drive as a Pest-Management Tool? -- 10.9. Hybrid Sterility -- 10.10. Medea in Tribolium -- 10.11. Cytoplasmic Agents Distort Normal Sex Ratios -- 10.11.1. Spiroplasma Strains -- 10.11.2.L-Form Bacteria -- 10.11.3. Rickettsia -- 10.11.4. Wolbachia -- 10.11.5. Cardinium -- 10.12. Paternal Sex-Ratio Chromosomes and Cytoplasmic Incompatibility in Nasonia -- 10.13. Male Killing in the Coccinellidae -- 10.14. Sex and the Sorted Insects -- 10.14.1. Genetic Control -- 10.14.2. Genetic Improvement of Parasitoids -- 10.15. Conclusion -- References Cited -- ch. 11 Molecular Genetics of Insect Behavior -- 11.1. Overview -- 11.2. Introduction -- 11.3. The Insect Nervous System -- 11.4. Traditional Genetic Analyses of Behavior -- 11.4.1. Crossing Experiments -- 11.4.2. Selection Experiments -- 11.4.3. Some Polygenically Determined Behaviors -- 11.5. Molecular-Genetic Analyses of Insect Behavior -- 11.5.1. The Photoperiodic Clock -- 11.5.2. Learning in Drosophila -- 11.5.3. Functional Genomics of Odor Behavior in Drosophila -- 11.5.4. Behavior of Apis mellifera -- 11.5.5. Pheromones in Insects -- 11.5.6. Neurobiochemistry of Drosophila -- 11.5.7. Divergent Functions of Est-6 and Est-5 in Two Drosophila Species: A Cautionary Tale of Homologs -- 11.5.8. Courtship Behavior in Drosophila -- 11.5.9. Speciation Genes in Drosophila and Other Insects -- 11.5.10. Personality in Insects: Tribolium confusum, Apis mellifera, Acyrthosiphon pisum, and Pyrrhocoris apterus -- 11.6. Symbionts and Insect Behavior -- 11.7. Human Neurodegenerative Diseases and Addictions in Drosophila -- 11.8. High-Throughput Ethomics -- 11.9. Systems Genetics of Complex Traits in Drosophila -- 11.10. Social Behavior in Bees and Ants -- 11.11. Conclusions -- References Cited -- ch. 12 Molecular Systematics and the Evolution of Arthropods -- 12.1. Overview -- 12.2. Introduction -- 12.3. Controversies in Molecular Systematics and Evolution -- 12.3.1. Molecular versus Morphological Traits -- 12.3.2. The Molecular Clock -- 12.3.3. The Neutral (or Nearly Neutral) Theory of Evolution -- 12.3.4. Homology and Similarity -- 12.4. Molecular Methods for Molecular Systematics and Evolution -- 12.4.1. Protein Electrophoresis -- 12.4.2. Molecular Cytology -- 12.4.3. Restriction Fragment Length Polymorphism (RFLP) Analysis -- 12.4.4. DNA and Genome Sequencing -- 12.4.5. Fragment Analyses of Genomic DNA -- 12.5. Targets of DNA Analysis -- 12.5.1. Mitochondria -- 12.5.2. Ribosomal RNA -- 12.5.3. Satellite DNA -- 12.5.4. Introns -- 12.5.5. Nuclear Protein-Coding Genes -- 12.5.6. Rare Genomic Changes -- 12.5.7. MicroRNAs -- 12.6. Steps in Phylogenetic Analysis of DNA Sequence Data -- 12.6.1. Gene Trees or Species Trees -- 12.6.2. Rooted or Unrooted Trees -- 12.6.3. Tree Types -- 12.6.4. Project Goals and Appropriate DNA Sequences -- 12.6.5. Sequence Comparisons with BLAST -- 12.6.6. Aligning Sequences -- 12.6.7. Constructing Phylogenies -- 12.6.8. Artifacts -- 12.6.9. Software Packages -- 12.7. The Universal Tree of Life -- 12.7.1. Two Domains -- 12.7.2. Three Domains -- 12.7.3. Origin of Eukaryota -- 12.8. The Fossil Record of Arthropods -- 12.9. Molecular Analyses of Arthropod Phylogeny -- 12.9.1. Evolution of the Ecdysozoa -- 12.9.2. Relationships among the Arthropoda -- 12.9.3. The Phylogeny of the Holometabola -- 12.9.4. Congruence Between Morphology- and Molecular-Based Trees -- 12.9.5. Genomes and Arthropod Phylogenies -- 12.10. Molecular Evolution and Speciation -- 12.10.1. Species Concepts -- 12.10.2. How Many Genes are Involved in Speciation? -- 12.10.3. Detecting Cryptic Species -- 12.11. Some Conclusions -- Relevant Journals -- References Cited -- ch. 13 Insect Population Ecology and Molecular Genetics -- 13.1. Overview -- 13.2. Introduction -- 13.3. What is Molecular Ecology? -- 13.4. Collecting Arthropods in the Field for Analysis -- 13.5. Molecular Ecological Methods -- 13.5.1. Allele-Specific PCR -- 13.5.2. Allozymes (Protein Electrophoresis) -- 13.5.3. Amplified Fragment Length Polymorphisms (AFLP-PCR) -- 13.5.4. Double-Strand Conformation Polymorphism (DSCP) -- 13.5.5. Heteroduplex Analysis (HDA) -- 13.5.6. Microarrays -- 13.5.7. Microsatellites -- 13.5.8. RFLP Analysis -- 13.5.9. PCR-RFLP -- 13.5.10. RAPD-PCR -- 13.5.11. Sequencing -- 13.5.12. Single Nucleotide Polymorphism (SNP) Markers -- 13.6. Analysis of Molecular Data -- 13.6.1. Allozymes -- 13.6.2. Microsatellites -- 13.6.3. RAPD-PCR -- 13.6.4. RFLPs -- 13.6.5. Sequencing -- 13.7. Case Studies in Molecular Ecology and Population Biology -- 13.7.1. Genetic Variability in the Fall Army worm: Incipient Species or Multiple Species? -- 13.7.2. Analyses of Natural Enemies -- 13.7.3. Population Isolation and Introgression in Periodical Cicadas -- 13.7.4. Eradicating Medflies in California? -- 13.7.5. Plant Defenses to Insect Herbivory -- 13.7.6. Origins of Insect Populations -- 13.8. Applied Pest Management -- 13.8.1. Monitoring Biotypes, Species, and Cryptic Species -- 13.8.2. Monitoring Vectors of Disease -- 13.8.3. Pesticide Resistances and Pest Management -- 13.8.4. Monitoring Pest-Population Biology -- 13.8.5. The "So What?" Test -- Relevant Journals -- References Cited -- ch. 14 Genetic Modification of Pest and Beneficial Insects for Pest-Management Programs -- 14.1. Overview -- 14.2. Introduction -- 14.3. Why Genetically Modify Insects? -- 14.3.1. Beneficial Insects -- 14.3.2. Pest Insects -- 14.4. Why Use Molecular-Genetic Methods? -- 14.5. What Genetic Modification Methods are Available? -- 14.5.1. Transposable-Element (TE) Vectors and Transgenesis -- 14.5.2. Paratransgenesis (Genetic Modification of Symbionts) -- 14.5.3. Viral Vectors -- 14.5.4. Transfer of Wolbachia from Another Arthropod -- 14.5.5. Site-Specific Modifications -- 14.5.6. No Vectors -- 14.5.7. RNAi to Control Pests -- 14.6. Methods to Deliver Exogenous Nucleic Acids into Arthropod Tissues -- 14.7. What Genes are Available? -- 14.8. Why are Regulatory Signals Important? -- 14.9. How are Modified Arthropods Identified? -- 14.10. How to Deploy Genetically Modified Pest and Beneficial Arthropods -- 14.11. | |
| 505 | 0 | |a Potential Risks Associated with Releases of Genetically Modified Arthropods -- 14.11.1. Could Gene Silencing Reduce Program Effectiveness? -- 14.11.2. Relative Risks -- 14.11.3. General Risk Issues -- 14.11.4. Horizontal Transfer (HT) -- 14.12. Permanent Releases of Genetically Modified Arthropods into the Environment -- 14.12.1. Models to Predict? -- 14.13. Regulatory Issues: Releases of Genetically Modified Arthropods -- 14.14. Conclusions -- References Cited. | |
| 520 | |a This book summarizes and synthesizes two rather disparate disciplines-entomology and molecular genetics. It provides an introduction to the techniques and literature of molecular genetics; defines terminology; and reviews concepts, principles, and applications of these powerful tools. | ||
| 590 | |a eBooks on EBSCOhost |b EBSCO eBook Subscription Academic Collection - Worldwide | ||
| 650 | 0 | |a Insects |x Molecular genetics. | |
| 650 | 0 | |a Arthropoda. | |
| 650 | 0 | |a Insects. | |
| 650 | 0 | |a Molecular structure. | |
| 650 | 2 | |a Arthropods | |
| 650 | 2 | |a Chemical Phenomena | |
| 650 | 2 | |a Phenomena and Processes | |
| 650 | 2 | |a Biochemical Phenomena | |
| 650 | 2 | |a Invertebrates | |
| 650 | 2 | |a Animals | |
| 650 | 2 | |a Eukaryota | |
| 650 | 2 | |a Organisms | |
| 650 | 2 | |a Insecta | |
| 650 | 2 | |a Molecular Structure | |
| 650 | 2 | |a Genetic Phenomena | |
| 650 | 4 | |a Zoology. | |
| 650 | 4 | |a Health & Biological Sciences. | |
| 650 | 4 | |a Invertebrates & Protozoa. | |
| 650 | 6 | |a Insectes |x Génétique moléculaire. | |
| 650 | 6 | |a Arthropodes. | |
| 650 | 6 | |a Insectes. | |
| 650 | 6 | |a Structure moléculaire. | |
| 650 | 7 | |a molecular structure. |2 aat | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Molecular Biology. |2 bisacsh | |
| 650 | 7 | |a Molecular structure. |2 fast |0 (OCoLC)fst01024846 | |
| 650 | 7 | |a Insects. |2 fast |0 (OCoLC)fst00974074 | |
| 650 | 7 | |a Arthropoda. |2 fast |0 (OCoLC)fst00817139 | |
| 650 | 7 | |a Insects |x Molecular genetics. |2 fast |0 (OCoLC)fst00974149 | |
| 776 | 0 | 8 | |i Print version: |a Hoy, Marjorie A. |t Insect molecular genetics. |b Third edition. |d Amsterdam : Academic Press, 2013 |z 9780124158740 |w (OCoLC)829055538 |
| 856 | 4 | 0 | |u https://ebsco.uam.elogim.com/login.aspx?direct=true&scope=site&db=nlebk&AN=486205 |z Texto completo |
| 880 | 0 | 0 | |6 505-01/(S |g 4.17. |t Evo-Devo and the Revolution in Developmental Studies -- |t References Cited -- |g pt. II |t MOLECULAR GENETIC TECHNIQUES -- |g ch. 5 |t Some Basic Tools: How to Cut, Paste, Copy, Measure, Visualize, and Clone DNA -- |g 5.1. |t Overview -- |g 5.2. |t Introduction to a Basic Molecular Biology Experiment -- |g 5.2.1. |t The Pros and Cons of Kits -- |g 5.2.2. |t A Simple Cloning Experiment -- |g 5.3. |t Extracting DNA from Insects -- |g 5.3.1. |t DNA Extraction Resulting in Loss of the Specimens -- |g 5.3.2. |t DNA Extraction That Does Not Require Destroying the Specimens -- |g 5.3.3. |t Assessing the Quality of Extracted DNA -- |g 5.4. |t Precipitating Nucleic Acids -- |g 5.5. |t Shearing DNA -- |g 5.6. |t Cutting DNA with Restriction Endonucleases -- |g 5.7. |t Joining DNA Molecules -- |g 5.8. |t Growth, Maintenance, and Storage of E. coli -- |g 5.9. |t Plasmids for Cloning in E. coli -- |g 5.10. |t Transforming E. coli with Plasmids -- |g 5.11. |t Purifying Plasmid DNA from E. coli -- |g 5.12. |t Electrophoresis in Agarose or Acrylamide Gels -- |g 5.13. |t Detecting, Viewing, and Photographing Nucleic Acids in Gels -- |g 5.14. |t Identifying Specific DNA by Southern Blot Analysis -- |g 5.15. |t Labeling DNA or RNA Probes -- |g 5.16. |t Removing DNA from Agarose Gels after Electrophoresis -- |g 5.17. |t Restriction-Site Mapping -- |t General References -- |t References Cited -- |g ch. 6 |t Some Additional Tools for the Molecular Biologist -- |g 6.1. |t Overview -- |g 6.2. |t Introduction -- |g 6.3. |t The Perfect Genomic Library -- |g 6.3.1. |t Lambda (λ) Phage as a Vector -- |g 6.3.2. |t Cloning with Cosmids -- |g 6.3.3. |t Cloning in the Filamentous Phage M13 -- |g 6.3.4. |t Phagemids -- |g 6.3.5. |t BACs -- |g 6.4. |t cDNA Cloning -- |g 6.5. |t Enzymes Used in Molecular Biology Experiments -- |g 6.6. |t Isolating a Specific Gene from a Library if Whole-Genome Sequencing is Not Done -- |g 6.7. |t Labeling Probes by a Variety of Methods -- |g 6.7.1. |t Synthesis of Uniformly Labeled DNA Probes by Random Primers -- |g 6.7.2. |t Synthesis of Probes by Primer Extension -- |g 6.7.3. |t End-Labeled Probes -- |g 6.7.4. |t Single-Stranded Probes -- |g 6.7.5. |t Synthetic Probes -- |g 6.8. |t Baculovirus Vectors Express Foreign Polypeptides in Insect Cells -- |g 6.9. |t Expression Microarray Analysis -- |t General References -- |t References Cited -- |g ch. 7 |t DNA Sequencing and the Evolution of the "-Omics" -- |g 7.1. |t Overview -- |g 7.2. |t Introduction -- |g 7.3. |t The Dideoxy or Chain-Termination (Sanger) Method -- |g 7.4. |t The Maxam and Gilbert Sequencing Method -- |g 7.5. |t Shotgun Strategies for Genomes -- |g 7.6. |t Sequencing DNA by the Polymerase Chain Reaction (PCR) -- |g 7.7. |t Automated Sanger Sequencers -- |g 7.7.1. |t Decreasing Costs of Sanger Sequencing -- |g 7.8. |t Analyzing DNA Sequence Data -- |g 7.9. |t DNA-Sequence Data Banks -- |g 7.10. |t A Brief History of the Drosophila Genome Project -- |g 7.10.1. |t The Original Drosophila Genome Project -- |g 7.10.2. |t The Actual Drosophila Genome Project -- |g 7.10.3. |t Drosophila Genome Analysis -- |g 7.10.4. |t Surprises in the Drosophila Genome -- |g 7.11. |t Next-Generation Sequencing Methods and Beyond -- |g 7.11.1. |t Next-Generation (NextGen or Second-Generation) Sequencing -- |g 7.11.2. |t Third-Generation Sequencing -- |g 7.12. |t Bioinformatics -- |g 7.12.1. |t Gene Ontology -- |g 7.13. |t Genome Analyses of Other Arthropods -- |g 7.13.1. |t Interesting Findings from Completed Genomes -- |g 7.13.2. |t What Do You Need to Do to Sequence Your Favorite Insect's Genome-- |g 7.14. |t Transposable Elements (TEs) as Agents of Genome Evolution -- |g 7.15. |t Transcriptomics -- |g 7.15.1. |t Tiling Microarrays -- |g 7.16. |t Metagenomics -- |g 7.17. |t Proteomics: Another "-Omic" -- |g 7.18. |t Functional Genomics -- |g 7.19. |t Structural Genomics---Another New Horizon-- |g 7.20. |t Comparative Genomics -- |g 7.21. |t Interactomes or Reactomes -- |g 7.22. |t The Post-Genomic Era: Systems Genetics -- |t General References -- |t References Cited -- |g ch. 8 |t DNA Amplification by the Polymerase Chain Reaction: Molecular Biology Made Accessible -- |g 8.1. |t Overview -- |g 8.2. |t Introduction -- |g 8.3. |t The Basic Polymerase Chain Reaction (PCR) -- |g 8.3.1. |t The First Few Cycles are Critical -- |g 8.3.2. |t PCR Power -- |g 8.3.3. |t Standard PCR Protocols -- |g 8.3.4. |t DNA Polymerases -- |g 8.3.5. |t Other Thermostable DNA Polymerases -- |g 8.3.6. |t Primers are Primary -- |g 8.3.7. |t Storing Insects for the PCR -- |g 8.3.8. |t Preparing DNA Samples -- |g 8.3.9. |t PCR Automation -- |g 8.3.10. |t Specificity of the PCR -- |g 8.3.11. |t Detecting Primer Artifacts -- |g 8.3.12. |t How Many Cycles Does a PCR Need-- |g 8.3.13. |t Reducing the Evils of Contamination -- |g 8.4. |t Some Modifications of the PCR -- |g 8.4.1. |t AFLP for DNA Fingerprinting -- |g 8.4.2. |t Anchored PCR -- |g 8.4.3. |t Arbitrary Primers -- |g 8.4.4. |t Asymmetric PCR -- |g 8.4.5. |t Degenerate Primers -- |g 8.4.6. |t Hot-Start PCR -- |g 8.4.7. |t Inverse PCR -- |g 8.4.8. |t Long PCR or High-Fidelity PCR -- |g 8.4.9. |t Multiplex PCR -- |g 8.4.10. |t Nested PCR -- |g 8.4.11. |t PCR-RFLP -- |g 8.4.12. |t Quantitative PCR -- |g 8.4.13. |t Random Primers. |
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