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Advances in genetics research. Volume 8 /
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
New York :
Nova Science Publishers, Inc.,
2012.
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| Colección: | Advances in Genetics Research.
|
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- ADVANCES IN GENETICS RESEARCH; VOLUME 8; ADVANCES IN GENETICS RESEARCH; ADVANCES IN GENETICS RESEARCH; VOLUME 8; Library of Congress Cataloging-in-Publication Data; CONTENTS; PREFACE; CHAPTER 1. GENETIC MODIFICATION OF STEM CELLSIN EX VIVO GENE THERAPY; ABSTRACT; 1. METHODS OF GENETIC MODIFICATIONS OF STEM CELLS; 1.1. Vector Constructions; 1.2. Viral Transduction of Stem Cells; Retroviruses; Lentiviruses; Adenoviruses; Adeno-Associated Viruses (AAV); 1.3. Non-Viral Stem Cell Modifications; 2. TRENDS IN GENETIC MODIFICATION OF STEM CELLS; 2.1. Directional Differentiation.
- 2.2. The Survival of Stem Cells in in vivo Conditions2.3. Delivery of Therapeutic Agents and Organ Regeneration in situ; 2.4. Anticancer Therapy; 3. SUMMARY; REFERENCES; CHAPTER 2. VIRAL RNA POLYMERASES; ABSTRACT; INTRODUCTION; BIOCHEMICAL FEATURES OF VIRALRNA POLYMERASES; INFLUENZA VIRUS RNA POLYMERASE HETEROTRIMER; HCV NS5B POLYMERASE; ENTEROVIRUS 71 3D POLYMERASE; REFERENCES; CHAPTER 3. POLYMERASE CHAIN REACTION: TYPES, UTILITIES AND LIMITATIONS; ABSTRACT; 1. TYPES, UTILITIES AND LIMITATIONS OF PCR; Multiplex PCR; Nested PCR; Reverse Transcriptase PCR (RT-PCR); Semiquantitative PCR.
- Real Time PCR2. APPLICATIONS OF PCR AND IMPACT ON SCIENCE; Medicine; Forensic Science; Agricultural Sciences and Environment; 3. CONCLUSIONS; REFERENCES; CHAPTER 4. GENETIC MODIFICATION TO IMPROVE THE THERAPEUTIC POTENTIAL OF ONCOLYTIC CLOSTRIDIA; ABSTRACT; 1. INTRODUCTION; 2. RECENT UNDERSTANDING OF THE PATHO-PHYSIOLOGY OF SOLID TUMOURS; 2.1. Tumour Angiogenesis and Hypoxia; 2.2. Tumour Structure, Cell Heterogeneity, and Anaerobic Metabolism; 3. ANAEROBIC CLOSTRIDIA AS ANTI-CANCER AGENTS; 3.1. Clostridia Selectively Colonise and Multiply in Solid Tumour.
- 3.2. Genetic Modification of Clostridial Strains4. CONCLUSION; 5. FUTURE DIRECTIONS; ACKNOWLEDGMENTS; REFERENCES; CHAPTER 5. TYPES AND APPLICATIONS OF DNA POLYMERASES; ABSTRACT; TAQ (THERMUS AQUATICUS) DNA POLYMERASE; 9 N M DNA POLYMERASE; BST (BACILLUS STEAROTHERMOPHILUS) DNA POLYMERASE; PFU (PYROCOCCUS FURIOSUS) DNA POLYMERASE; TTH (THERMUS THERMOPHILUS) DNA POLYMERASE; T4 DNA POLYMERASE; VENT DNA POLYMERASE; DEEP VENTR DNA POLYMERASE; DNA POLYMERASE I AND KLENOW FRAGMENT; HERCULASE DNA POLYMERASE; PHI29 DNA POLYMERASE; SEQUENASE DNA POLYMERASE; THERMINATOR DNA POLYMERASE; REFERENCES.
- CHAPTER 6. THERMOSTABLE DNA POLYMERASES: MOLECULAR CLONING AND EVOLUTIONABSTRACT; INTRODUCTION; MATERIALS AND METHODS; Bacterial Strains, Vectors and Culture Conditions; Genomic DNA Extraction; 16S rDNA Analysis; Molecular Phylogenetic Analysis and Primer Design; Cloning and Sequencing of DNA Polymerase I Genes; Sequence Analysis; RESULTS AND DISCUSSION; Phylogenetic Analysis; Cloning of DNA Pol I Gene; Bioinfomatic Analysis; ACKNOWLEDGMENT; REFERENCES; CHAPTER 7. CLONING OF THE b2-MICROGLOBULIN FROM SEA BASS (DICENTRARCHUS LABRAX L.): MOLECULAR CHARACTERISATION AND EXPRESSION STUDIES.