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Fluorescence probes in oncology /
A comprehensive description of fluorescence probes and the methodology for the study and diagnostics of oncology. The material is drawn directly from the work of pioneer researchers in cell biology and pathology, and offers a perspective of their crucial investigations and lifetime experiences; it a...
| Clasificación: | Libro Electrónico |
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| Autor principal: | |
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
London : River Edge, NJ :
Imperial College Press ; Distributed by World Scientific Pub.,
©2002.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Ch. I. The nature of light. 1.1. Origins. 1.2. Refraction. 1.3. Fermat's principle. 1.4. The speed of light. 1.5. The wave nature of light. 1.6. Diffraction and resolution limits. 1.7. The quantum theory and photons. 1.8. The production of light. 1.9. The Planck equation and photochemical reactions
- ch. II. Introduction to photophysics. 2.1. From atoms to molecules: the formation of chemical bonds. 2.2. Electronic transitions. 2.3. Excitation and deactivation of molecules. 2.4. Factors influencing the fluorescence quantum yield. 2.5. Quenching of the first excited singlet state by energy transfer
- ch. III. Basic approaches to the experimental study of chemical and biological luminescence. 3.1. Long-lifetime probes: metal-ligand complexes as a new class of long-lived fluorophores for protein hydrodynamics. 3.2. Chemiluminescence. 3.3. Bioluminescence. 3.4. Green fluorescent protein (GFP)
- ch. IV. Methods and instrumentation. 4.1. Spectrofluorometers and cell sorters. 4.2. Microspectrofluorometers. 4.3. Detectors used in fluorescence imaging. 4.4. Fluorescence imaging with CCD cameras. 4.5. Digital imaging. 4.6. Ratio fluorescence microscopy. 4.7. Multiparameter imaging. 4.8. Multiprobe approach to the study of intercellular communication. 4.9. Photodiode array fluorescence spectroscopy. 4.10. Confocal fluorescence microscopy. 4.11. Fluorescence lifetime imaging (FLIM). 4.12. Frequency-domain spectroscopy and tomography of tissues. 4.13. Fluorescence photobleaching recovery (FPR, also FRAP) and green fluorescent protein (GFP) chimeras. 4.14. Time-resolved fluorescence resonance energy transfer (FRET). 4.15. Fluorescence polarization in the microscope. 4.16. Polarization fluorescence photobleaching recovery (PFPR). 4.17. Picosecond fluorescence kinetics. 4.18. Raman fluorescence spectroscopy in single living cells. 4.19. Experimental time-resolved methods and photon migration in tissues studied by time-resolved spectroscopy. 4.20. Fourier transform spectroscopy in the infrared. 4.21. Secondary ion mass spectrometry (SIMS).
- Ch. V. New methods. Theoretical basis and potential applications to biology. 5.1. Total internal reflection fluorescence microscopy (TIRF) in cells. 5.2. Surface fluorescence detection by a microscope. 5.3. Intracellular sensing and optical imaging beyond the diffraction limit: scanning near-field optical microscopy (SNOM). 5.4. Optical forces, optical traps, optical tweezers in combination with microspectroscopy and fluorescent DNA intercalating dyes. 5.5. Fourier interferometric stimulation (FIS) and spectral image analysis. 5.6. Fourier transform multiplex spectroscopy and spectral imaging of protoporphyrin in single melanoma cells. 5.7. Fluorescence in situ hybridization (FISH). 5.8. Beyond FISH: multicolor spectral karyotyping of human chromosomes (SKY). 5.9. Combinatorial multi-fluor FISH. 5.10. Two-photon excitation induced fluorescence. 5.11. Three-photon induced fluorescence. 5.12. Light fluorescence quenching (LQ) and stimulate emission (SE). 5.13. Related topics: infrared imaging spectroscopy of breast
- ch. VI. Fluorescent probes. 6.1. Ion probes. 6.2. Membrane potential probes. 6.3. Cell organelle probes. 6.4. Probes of cell metabolism in the living cell, as a receptacle of smart probes. 6.5. Probes of cyclic Amp (cAMP) probing, signaling and oscillations. 6.6. Nucleic acid probes. 6.7. Probes of oxygenation and anoxia. 6.8. Fluorescent probes of reduced glutathione and thiol groups. 6.9. Merocyanines. 6.10. Caged substrates and photoactivated probes. 6.11. Optical thermometry in single living cells
- ch. VII. Applications of fluorescence techniques to study biological processes in normal and pathological cells. 7.1. Cell metabolism. 7.2. Signal transduction
- ch. VIII. Cell physiopathology. 8.1. Brain tumors. 8.2. Pituitary tumors. 8.3. Breast tumors. 8.4. Head and neck tumors. 8.5. Cancer of the cervix. 8.6. Lung and lung tumors. 8.7. Prostate cancer. 8.8. Cancer of the colon. 8.9. Sarcoma. 8.10. Immunoperoxidase studies of tumors. 8.11. Tumors with images of the characteristic immunoperoxidase reaction. 8.12. Viral pathology. 8.13. AIDS.