GFP whole cell microbial biosensors : scale-up and scale-down effects on biopharmaceutical processes /

Two strategies are usually considered for the optimization of microbial bioprocesses. The first one involves genetic or metabolic engineering of the target microbial strains in order to improve its production efficiency or its tolerance to adverse conditions. The second one is based on the chemical...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Delvigne, Frank
Formato: Electrónico eBook
Idioma:Inglés
Publicado: New York, N.Y. : New York, N.Y. (222 East 46th Street, New York, NY 10017) : ASME ; Momentum Press, 2013.
Colección:Biomedical & nanomedical technologies.
Temas:
Microbial biotechnology.
Green fluorescent protein.
Biosensors.
Biotechnologie microbienne.
Protéine fluorescente verte.
Biocapteurs.
MEDICAL > Microbiology.
biomedical and nanomedical technologies
engineering
microbial cells
Acceso en línea:Texto completo
Tabla de Contenidos:
  • 1. Interaction between fluid flow and microbial cells: importance of the operating scale
  • 1.1 Different scales involved in the development of microbial bioprocesses
  • 1.2 Hydrodynamic modeling of the substrate heterogeneites perceived by cells.
  • 2. Stochastic simulation of the displacement of microbial cells along concentration field
  • 2.1 Microbial stress response: different timescales involved.
  • 3. Experimental results gained from the physiological response of GFP biosensors in scale-down conditions
  • 3.1 General stress response in scale-down bioreactors: rpoS::GFP
  • 3.2 Use of destabilized GFP variants.
  • 4. Another source of information: protein leakage and the study of the secretome
  • References.

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