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Transgenic animal technology : a laboratory handbook /
Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer - from molecular methods to whole animal cons...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam ; Boston :
Academic Press,
©2002.
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| Edición: | 2nd ed. |
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 245 | 0 | 0 | |a Transgenic animal technology : |b a laboratory handbook / |c edited by Carl A. Pinkert. |
| 250 | |a 2nd ed. | ||
| 260 | |a Amsterdam ; |a Boston : |b Academic Press, |c ©2002. | ||
| 300 | |a 1 online resource (xv, 618 pages :) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 504 | |a Includes bibliographical references and index. | ||
| 520 | |a Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer - from molecular methods to whole animal considerations - for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. Contains a comprehensive collection of transgenic animal and gene transfer methods Discusses background and introduction to techniques and animal systems Teaches practical step-by-step protocols New section on analysis. | ||
| 505 | 0 | |a Front Cover; Transgenic Animal Technology: A Laboratory Handbook; Copyright Page; Table of Contents; Contributors; Preface; PART 1: Overview; Chapter 1. Introduction to Transgenic Animal Technology; I. Introduction; II. Historical Background; III. Applications and Overview of Text; References; PART 2: Transgenic Animal Production Focusing on the Mouse Model; Chapter 2. DNA Microinjection and Transgenic Animal Production; I. Introduction; II. General Methods; III. Summary; Acknowledgments; References; Appendix; Chapter 3. Factors Affecting Transgenic Animal Production; I. Introduction. | |
| 505 | 8 | |a II. Getting StartedIII. Troubleshooting; IV. Transgenic Phenomenology; V. Summary; Acknowledgments; References; Chapter 4. Gene Targeting in Embryonic Stem Cells:; I. Stem Cells of the Embryo; II. Gene Targeting in Embryonic Stem Cells; III. Methods; Acknowledgments; References; Chapter 5. Gene Targeting in Embryonic Stem Cells:; I. Introduction; II. Conditional Technologies; III. Cre-loxP System Review, Development and Testing; IV. Cre-loxP and Transgenic Models; V. Methods; VI. Summary; Acknowledgments; References; Chapter 6. Retrovirus-Mediated Gene Transfer Teoan Kim. | |
| 505 | 8 | |a I. Introduction and DiscussionII. Methods; Acknowledgment; References; Chapter 7. Nuclear Transfer Technologies Yukio Tsunoda and Yoko Kato; I. Introduction and Discussion; II. Materials and Equipment; III. Preparation of Microtools; IV. Nuclear Transfer of Preimplantation Embryos; V. Nuclear Transfer of Embryonic Stem Cells and Somatic Cells; Acknowledgment; References; PART 3: Production of Transgenic Laboratory and Domestic Animal Species; Chapter 8. Production of Transgenic Rats; I. Introduction; II. Importance of Rats; III. Contributions from Transgenic Rats; IV. Production. | |
| 505 | 8 | |a V. ConclusionsReferences; Chapter 9. Production of Transgenic Rabbits; I. Applications of Transgenic Rabbits; II. Manipulation of Rabbit Embryos for Production of Transgenics; III. Materials and Methods; References; Chapter 10. Production of Transgenic Fish; I. Introduction; II. Discussion; III. Methods of Gene Transfer; IV. Establishing and Maintaining Transgenic Lineages; V. Care and Containment of Transgenic Fish; VI. Future of the Transgenic Fish; Acknowledgments; References; Chapter 11. Production of Transgenic Poultry; I. Introduction and Discussion; II. Methods; III. Summary. | |
| 546 | |a English. | ||
| 590 | |a eBooks on EBSCOhost |b EBSCO eBook Subscription Academic Collection - Worldwide | ||
| 650 | 0 | |a Animal genetic engineering |v Laboratory manuals. | |
| 650 | 6 | |a Génie génétique animal |v Manuels de laboratoire. | |
| 650 | 7 | |a TECHNOLOGY & ENGINEERING |x Agriculture |x Animal Husbandry. |2 bisacsh | |
| 650 | 7 | |a Animal genetic engineering. |2 fast |0 (OCoLC)fst00809192 | |
| 655 | 2 | |a Laboratory Manual | |
| 655 | 7 | |a Laboratory manuals. |2 fast |0 (OCoLC)fst01920776 | |
| 655 | 7 | |a Laboratory manuals. |2 lcgft | |
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| 700 | 1 | |a Pinkert, Carl A. | |
| 776 | 0 | 8 | |i Print version: |a Pinkert, Carl A. |t Transgenic Animal Technology : A Laboratory Handbook. |d Burlington : Elsevier Science, ©2002 |z 9780125571661 |
| 856 | 4 | 0 | |u https://ebsco.uam.elogim.com/login.aspx?direct=true&scope=site&db=nlebk&AN=551483 |z Texto completo |
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