Molecular biology techniques : a classroom laboratory manual /

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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hand...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: Carson, Sue (Susan)
Otros Autores: Miller, Heather B., Witherow, D. Scott
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Amsterdam ; Boston : Elsevier /Academic Press, ©2012.
Edición:3rd ed.
Colección:ClinicalKey.
Temas:
Molecular biology > Laboratory manuals.
Molecular biology > Technique > Handbooks, manuals, etc.
Recombinant DNA.
Protein engineering.
DNA, Recombinant
Molecular Biology > methods
Protein Engineering
Biologie moléculaire > Manuels de laboratoire.
Biologie moléculaire > Technique > Guides, manuels, etc.
ADN recombinant.
Technologie des protéines.
SCIENCE > Life Sciences > Molecular Biology.
Recombinant DNA
Protein engineering
Molecular biology
Molecular biology > Technique
Methode
Molekularbiologie
Methode > (DE-588c)4038971-6.
Molekularbiologie > (DE-588c)4039983-7.
Handbook
Laboratory Manual
handbooks.
manuals (instructional materials)
Handbooks and manuals
Laboratory manuals
Handbooks and manuals.
Laboratory manuals.
Guides et manuels.
Manuels de laboratoire.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Lab Session 1 Getting Oriented: Practicing with Micropipettes
  • Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA
  • Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation
  • Lab Session 4 Preparation of Insert DNA (egfp) PCR Product
  • Lab Session 5 DNA Ligation and Transformation of Escherichia coli
  • Lab Session 6 Colony Hybridization
  • Lab Session 7 Characterization of Recombinant Clones: Part 1
  • Lab Session 8 Characterization of Recombinant Clones: Part 2
  • Lab Session 9 Characterization of Recombinant Clones: Part 3
  • Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2
  • Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1
  • Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2
  • Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column
  • Lab Session 14 Analysis of Purification Fractions
  • Lab Session 15 Total RNA Purification
  • Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1
  • Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2
  • Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1
  • Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2
  • Appendix 1 Equipment
  • Appendix 2 Prep List
  • Appendix 3 Preparation of Competent E. coli Cells
  • Appendix 4 Pre-Lab Questions.

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