Cargando…
An introduction to metabolic and cellular engineering /
Metabolic and cellular engineering, as presented in this book, is a powerful alliance of two technologies: genetics â€" molecular biology and fermentation technology. Both are driven by continuous refinement of the basic understanding of metabolism, physiology and cellular biology (growth, div...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
River Edge, NJ :
World Scientific,
©2002.
|
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Preface; Contents; List of Abbreviations; Introduction; Introductory Outlines; Metabolic and Cellular Engineering in the Context of Bioprocess Engineering; Tools for Metabolic and Cellular Engineering; Engineering Cells for Specific Biotransformations; Metabolic Areas that Have Been Subjected to MCE; From DNA Sequence to Biological Function; Temporal and Spatial Scaling in Cellular Processes; Scaling in Microbial and Biochemical Systems; Views of the Cell; Black and Grey Boxes: Levels of Description of Metabolic Behavior in Microorganisms; Transduction and Intracellular Signalling.
- Self-organized Emergent PhenomenaHomeodynamics and Coherence; Matter and Energy Balances; Mass Balance; General Formulation of Mass Balance; Integral and Differential Mass Balances; Growth Stoichiometry and Product Formation; Biomass and Product Yields; Electron Balance; Theoretical Oxygen Demand; Opening the ""Black Box"". Mass Balance as the Basis of Metabolic Flux Analysis; Energy Balance; Forms of Energy and Enthalpy; Calorimetric Studies of Energy Metabolism; Heat of Combustion; An Energetic View of Microbial Metabolism; Cell Growth and Metabolite Production. Basic Concepts.
- Microbial Growth under Steady and Balanced ConditionsMicrobial Energetics under Steady State Conditions; Growth Kinetics under Steady State Conditions; The Dilution Rate; The Dilution Rate and Biomass Concentration; The Dilution Rate and the Growth-limiting Substrate Concentration; Biomass and Growth-limiting Substrate Concentration at the Steady State; Growth as a Balance of Fluxes; The Flux Coordination Hypothesis; Toward a Rational Design of Cells; Redirecting Central Metabolic Pathways under Kinetic or Thermodynamic Control.
- Thermodynamic or Kinetic Control of Flux under Steady State ConditionsKinetic and Thermodynamic Limitations in Microbial Systems. Case Studies; Saccharomyces cerevisiae; Escherichia coli; Increasing Carbon Flow to Aromatic Biosynthesis in Escherichia coli; Methods of Quantitation of Cellular Processes Performance
- Stoichiometry of Growth: The Equivalence between Biochemical Stoichiometries and Physiological Parameters; A General Formalism for Metabolic Flux Analysis; A Comparison between Different Methods of MFA; MFA Applied to Prokaryotic and Lower Eukaryotic Organisms.
- MFA as Applied to Studying the Performance of Mammalian Cells in CultureMetabolic Fluxes during Balanced and Steady State Growth; Bioenergetic and Physiological Studies in Batch and Continuous Cultures. Genetic or Epigenetic Redirection of Metabolic Flux; Introduction of Heterologous Metabolic Pathways; Metabolic Engineering of Lactic Acid Bacteria for Optimising Essential Flavor Compounds Production; Metabolic Control Analysis; Summation and connectivity theorems; Control and Regulation; The Control of Metabolites Concentration.