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Stem cell bioengineering /
Written and edited by recognized experts in the field, the new Artech House Methods in Bioengineering book series offers detailed guidance on authoritative methods for addressing specific bioengineering challenges. Offering a highly practical presentation of each topic, each book provides research e...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Boston, Mass. ; London :
Artech House,
©2009.
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| Colección: | Artech House methods in bioengineering series.
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| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 245 | 0 | 0 | |a Stem cell bioengineering / |c Biju Parekkadan, Martin L. Yarmush, editors. |
| 260 | |a Boston, Mass. ; |a London : |b Artech House, |c ©2009. | ||
| 300 | |a 1 online resource (xiv, 208 pages) : |b illustrations (chiefly color) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
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| 490 | 1 | |a Methods in bioengineering series | |
| 504 | |a Includes bibliographical references and index. | ||
| 588 | 0 | |a Print version record. | |
| 520 | 3 | |a Written and edited by recognized experts in the field, the new Artech House Methods in Bioengineering book series offers detailed guidance on authoritative methods for addressing specific bioengineering challenges. Offering a highly practical presentation of each topic, each book provides research engineers, scientists, and students with step-by-step procedures, clear examples, and effective ways to overcome problems that may be encountered. This cutting-edge volume is focused on methods to derive, manipulate, target, and/or prepare stem cells for clinical use. The book helps you master powerful stem cell bioengineering methods, enabling them to rigorously test hypotheses and compare their results to "gold standards". |c Publisher abstract | |
| 505 | 0 | |a Methods in Bioengineering: Stem Cell Bioengineering; Contents; Preface; Chapter 1 Somatic Cell Nuclear Transfer and Derivation of Embryonic Stem Cells; 1.1 Introduction; 1.2 Materials for Nuclear Transfer; 1.2.1 Equipment for mouse nuclear transfer; 1.2.2 Reagents for mouse nuclear transfer; 1.3 Methods for Nuclear Transfer; 1.3.1 Preparation of enucleation and nuclear transfer pipettes; 1.3.2 Medium preparation; 1.3.3 Animal preparation; 1.3.4 Nuclear transfer; 1.3.5 Enucleation; 1.3.6 Preparation of donor cells; 1.3.7 Nuclear transfer; 1.3.8 Activation | |
| 505 | 8 | |a 1.3.9 Embryo culture and embryo transfer1.4 Derivation of Mouse ntES Cells; 1.5 Materials for Embryonic Stem Cell Derivation; 1.6 Methods for Embryonic Stem Cell Derivation; 1.6.1 Derivation of ntES cells; 1.6.2 In vitro characterization of ntES cells; 1.6.3 In vivo characterization of ntES cells; 1.7 Discussion and Commentary; 1.8 Summary Points; Acknowledgments; References; Chapter 2 Derivation of Mouse Parthenogenetic Embryonic Stem Cells; 2.1 Introduction; 2.2 Materials; 2.2.1 Reagents; 2.2.2 Equipment; 2.2.3 Media recipe; 2.3 Methods; 2.3.1 Generation of p(MI) embryos | |
| 505 | 8 | |a 2.3.2 Generation of p(MII) embryos2.3.3 Generation of p(hap) embryos; 2.3.4 Derivation of p(MI), p(MII), and p(hap) ES cells; 2.3.5 ES cell characterization; 2.3.6 Teratoma induction; 2.4 Data Acquisition, Anticipated Results, and Interpretation; 2.5 Discussion and Commentary; 2.6 Summary Points; Acknowledgments; References; Chapter 3 Generation of Mice from Embryonic Stem Cells Using Tetraploid Embryos as Hosts; 3.1 Introduction; 3.2 Experimental Design; 3.3 Materials; 3.3.1 Preparation of 4n embryos: Mice and reagents; 3.3.2 Preparation of 4n embryos: Equipment | |
| 505 | 8 | |a 3.3.3 ES cells and culture conditions3.3.4 Micromanipulation system; 3.4 Methods; 3.4.1 Preparation of host embryos; 3.4.2 Electrofusion of two-cell stage embryos; 3.4.3 Removal of the zona pellucida; 3.4.4 Generation of ES multiple 4n embryos by aggregation; 3.4.5 Generation of ES 4n embryos by blastocyst injection; 3.4.6 Data acquisition; 3.5 Anticipated Results; 3.6 Discussion and Commentary; 3.7 Application Notes; 3.8 Summary Points; Acknowledgments; References; Chapter4 Bioreactor Design and Implementation; 4.1 Introduction; 4.2 Experimental Methods and Materials | |
| 505 | 8 | |a 4.2.1 General system description4.2.2 Closed system; 4.2.3 Hollow-fiber bioreactor; 4.2.4 CES fluid circuit; 4.2.5 Oxygenator design; 4.2.6 Monitoring; 4.2.7 User interface; 4.3 Anticipated Results; 4.3.1 MSC source 1: Loading whole BM marrow into the CES; 4.3.2 MSC source 2 or 3: Loading preselected MSC into the CES; 4.4 Discussion and Commentary; 4.5 Application Notes; 4.5.1 Therapeutic dose of MSC grown from a whole BM sample; 4.5.2 Nonadherent cell culture: Kg1a cells grown in suspension; 4.6 Summary Points; Chapter 5 Extracellular Matrix Microarrays and Stem Cell Fate; 5.1 Introduction | |
| 590 | |a eBooks on EBSCOhost |b EBSCO eBook Subscription Academic Collection - Worldwide | ||
| 650 | 0 | |a Stem cells. | |
| 650 | 0 | |a Stem cells |x Research. | |
| 650 | 0 | |a Bioengineering. | |
| 650 | 2 | |a Biomedical Engineering |x methods | |
| 650 | 2 | |a Stem Cells | |
| 650 | 2 | |a Biotechnology | |
| 650 | 6 | |a Cellules souches. | |
| 650 | 6 | |a Cellules souches |x Recherche. | |
| 650 | 6 | |a Biotechnologie. | |
| 650 | 7 | |a bioengineering. |2 aat | |
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| 650 | 7 | |a Bioengineering. |2 fast |0 (OCoLC)fst00832028 | |
| 650 | 7 | |a Stem cells. |2 fast |0 (OCoLC)fst01132975 | |
| 650 | 7 | |a Stem cells |x Research. |2 fast |0 (OCoLC)fst01132977 | |
| 700 | 1 | |a Parekkadan, Biju. | |
| 700 | 1 | |a Yarmush, Martin L. | |
| 776 | 0 | 8 | |i Print version: |t Stem cell bioengineering. |d Boston, Mass. ; London : Artech House, ©2009 |z 9781596934023 |w (OCoLC)437082380 |
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