RNA methodologies : a laboratory guide for isolation and characterization /

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This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA st...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: Farrell, Robert E., Jr
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Amsterdam ; Boston : Elsevier/Academic Press, ©2005.
Edición:3rd ed.
Temas:
RNA > Analysis > Laboratory manuals.
RNA > isolation & purification
Molecular Biology > methods
RNA > physiology
ARN > Analyse > Manuels de laboratoire.
SCIENCE > Life Sciences > Molecular Biology.
RNA > Analysis.
RNA.
Laboratoriumonderzoek.
Laboratory Manual
Laboratory manuals.
Manuels de laboratoire.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • RNA and the cellular biochemistry revisited
  • Transcription and the organization of eukaryotic genes
  • Messenger RNA
  • Resilient ribonucleases
  • RNA isolation strategies
  • The truth about tissues
  • Isolation of polyadenylated RNA
  • Quality control for RNA preparations
  • Dot blot analysis
  • Electrophoresis of RNA
  • Photodocumentation and image analysis
  • Northern analysis
  • Nucleic acid probe technology
  • Practical nucleic acid hybridization
  • Principles of detection
  • Quantification of specific mRNAs by nuclease protection
  • Analysis of nuclear RNA
  • cDNA synthesis
  • RT-PCR
  • Quantitative PCR techniques
  • Transcript subtraction methods
  • mRNA differential display
  • High-throughput analysis of gene expression
  • RNA interference : targeted gene silencing
  • Genomes, transcriptomes, proteomes, and bioinformatics
  • An RNA Paradigm
  • Epilogue : a few pearls of wisdom.

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