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Membrane protein purification and crystallization : a practical guide /
This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam ; Boston :
Academic Press,
©2003.
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| Edición: | 2nd ed. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- I. Strategies and Techniques
- Purification Strategies for Membrane Proteins.
- Chromatographic Techniques and Basic Operations in Membrane Protein Purification.
- Production and purification of recombinant membrane proteins.
- SDS Electrophoresis Techniques.
- Blue-Native Electrophoresis.
- Preparative Isoelectric Focussing.
- Membrane protein crystallization.
- II. Discussion of Selected Isolation Protocols
- Lipid Dependent Inactivation and Reactivation of Bovine complex III.
- Purification of affinity-epitope tagged G-protein coupled receptors.
- Purification of NhaA, the Na+/H+ Antiporter of Escherichia coli for 3D or 2D
- Crystallization.
- Purification of the yeast cytochrome bc1 complex.
- III. Crystallization of Membrane Proteins
- Antibody fragment-mediated crystallization of membrane proteins.
- Crystallization of Wolinella succinogenes quinol:fumarate reductase.
- Ba3-type cytochrome-c oxidase from Thermus thermophilus:
- purification, crystallization, and crystal transformation.
- Two-dimensional crystallization of membrane proteins: a practical guide.
- In cubo crystallization of membrane proteins.