Biotechnology : a laboratory course /

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The objectives of this Second Edition of Biotechnology: A Laboratory Course remain unchanged: to create a text that consists of a series of laboratory exercises that integrate molecular biology with protein biochemistry techniques while providing a continuum of experiments. The course begins with ba...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: Becker, Jeffrey M.
Otros Autores: Caldwell, Guy A., Zachgo, Eve Ann
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego : Academic Press, ©1996.
Edición:2nd ed.
Temas:
Biotechnology > Laboratory manuals.
Biotechnology
Biotechnologie > Manuels de laboratoire.
Biotechnologie.
bioengineering.
SCIENCE > Biotechnology.
Biotechnology.
Laboratory Manual
Laboratory manuals.
Manuels de laboratoire.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Suggested Schedule for Laboratory Exercises.
  • Introductory Notes.
  • Record Keeping and Safety Rules.
  • Format of Student Laboratory Records.
  • The Ten Commandments of Record Keeping.
  • Safety Rules in the Laboratory.
  • Exercise 1: Aseptic Culture and Establishing Pure Cultures.
  • Exercise 2: Preparation of Culture Media.
  • Exercise 3: The Growth Curve.
  • Exercise 4: Isolation of Plasmid DNA from Escherichia coli: The Mini-Prep.
  • Exercise 5: Purification, Concentration, and Quantitation of DNA.
  • Exercise 6: Large-Scale Isolation of Plasmid DNA by Column Chromatography.
  • Exercise 7: Amplification of a lacZ.
  • Gene Fragment by the Polymerase Chain Reaction.
  • Exercise 8: Restriction Digestion and Agarose Gel Electrophoresis.
  • Exercise 9: Southern Transfer.
  • Exercise 10: Preparation, Purification, and Hybridization of Probe.
  • Exercise 11: Transformation of Saccharomyces cerevisiae.
  • Exercise 12: Isolation of Plasmid from Yeast and Escherichia coli Transformation.
  • Exercise 13: Protein Assays.
  • Exercise 14: Qualitative Assay for Galactosidase in Yeast Colonies.
  • Exercise 15: Determination of Galactosidase in Permeabilized Yeast Cells.
  • Exercise 16: Assay of Galactosidase in Cell Extracts.
  • Exercise 17: Galactosidase Purification.
  • Exercise 18: Western Blot: Probe of Protein Blot with Antibody to Galactosidase.
  • Appendix 1: Alternate Protocols and Experiments:
  • Exercise 1A: Isolation and Characterization of Auxotrophic Yeast Mutants.
  • Exercise 2A: Measurement of pH.
  • Exercise 3A: Use of the Spectrophotometer.
  • Exercise 6A: Isolation of Plasmid DNA: The Maxi-Prep.
  • Exercise 10A: Colony Hybridization.
  • Appendix 2: Buffer Solutions.
  • Appendix 3: Preparation of Buffers and Solutions.
  • Appendix 4: Properties of Some Common Concentrated Acids and Bases.
  • Appendix 5: Use of Micropipettors.
  • Appendix 6: Safe Handling of Microorganisms. Appendix 7: List of Cultures.
  • Appendix 8: Storage of Cultures and DNA.
  • Appendix 9: Sterilization Methods.
  • Appendix 10: Preparation of Stock Solutions for Culture Media.
  • Appendix 11: Growth in Liquid Medium.
  • Appendix 12: Determination of Viable Cells.
  • Appendix 13: Determination of Cell Mass.
  • Appendix 14: Determination of Cell Number.
  • Appendix 15: Nomenclature of Strains.
  • Appendix 16: Glassware and Plasticware.
  • Appendix 17: Preparation of Tris and EDTA.
  • Appendix 18: Basic Rules for Handling Enzymes.
  • Appendix 19: Effects of Common Contaminants on Protein Assays.
  • Appendix 20: Manufacturers' and Distributors' Addresses.
  • Appendix 21: Surfing the Bionet: World Wide Web Addresses.
  • Glossary.
  • Subject Index.

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