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RNA viruses : a practical approach /
RNA Viruses: A Practical Approach provides a broad treatment of the principles and practice of RNA virus research to ensure the widest possible audience. RNA Viruses: A Practical Approach will be of interest to all laboratories involved in virus culture, including both academic and industrial resear...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Oxford ; New York :
Oxford University Press,
2000.
|
| Colección: | Practical approach series ;
226. |
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 245 | 0 | 0 | |a RNA viruses : |b a practical approach / |c edited by Alan J. Cann. |
| 260 | |a Oxford ; |a New York : |b Oxford University Press, |c 2000. | ||
| 300 | |a 1 online resource (xx, 266 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
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| 490 | 1 | |a The practical approach series ; |v 226 | |
| 504 | |a Includes bibliographical references and index. | ||
| 588 | 0 | |a Print version record. | |
| 505 | 0 | |a Intro; Contents; List of protocols; Abbreviations; 1 Investigation of RNA virus genome structure; 1 Introduction-the nature of the virus genome; Properties of the genomes of RNA viruses; Properties of the virions of RNA viruses; Paradigm for analysis of RNA virus genome structure; 2 Growth, assay, and purification of RNA viruses; Source of virus: in vivo versus in vitro methods; Assay of virus yield; Harvesting and concentrating of virus; Purification of virus; Radiolabelling; 3 RNA extraction; Genome RNA extraction from virions; Genome RNA extraction without virion purification | |
| 505 | 8 | |a 4 Fractionation of RNA, and analysis by Northern (RNA) blottingFractionation of RNA; Northern blotting and detection with labelled probes; 5 Further analysis; Characterization by restriction-endonuclease digest patterns; Characterization by ribonuclease protection; References; 2 Mutagenesis of RNA virus genomes; 1 Introduction; 2 Generation of RNA virus mutants; Selection of mutants from the RNA quasispecies; Recombination of RNA virus genomes; Reassortment of segmented RNA virus genomes; Generation of defective RNA virus populations by passage in culture | |
| 505 | 8 | |a Recovery of RNA virus mutants from infectious cDNA3 Determining the genotype of RNA virus mutants; 4 Analysis of the frequency of RNA virus mutants; References; 3 Analysis of transcriptional control In RNA virus Infections; 1 Introduction; 2 Analysis of virus mRNAs in virus-infected cells; General considerations; Radiolabelling and extraction of virus RNAs; Gel electrophoresis of RNAs; 3 Analysis of virus mRNA structure; General considerations; Separation of virus RNA in preparative agarose gels; Extraction of virus RNA from gel slices; One-dimensional oligonucleotide fingerprinting | |
| 505 | 8 | |a Northern blot hybridization4 The use of RNA reporter constructs for transcriptional assays; RNA transcription in vitro; RNA transfection of DI RNA construct and helper virus infection; DNA transfection and vaccinia virus infection; CAT reporter assay system; Detection and quantitation of minute amount of virus RNAs; References; 4 Analysis of RNA virus-encoded proteinases; 1 Introduction; 2 Defining the proteinase type; Inhibitor studies; Sequence analysis; 3 Delimiting the proteinase (domain); Deletion/truncation analysis; Screening for proteolytic activity using translation systems in vitro | |
| 505 | 8 | |a 4 Bacterial expressionExpression of inactive proteinase; Affinity 'tagging'; Insolubility of expressed proteinases; 5 Substrate specificity; 6 Artificial 'reporter' polyproteins; References; 5 Detection and analysis of host gene targets for oncogenic retroviruses; 1 Introduction; 2 Insertional mutagenesis; Analysis of virus integration patterns; Cloning of virus integration sites; Analysis of host DNA flanking the provirus integration sites; Location of genes at integration sites; 3 Transduction of virus oncogenes; Properties of transducing viruses; Biological methods of detection | |
| 520 | |a RNA Viruses: A Practical Approach provides a broad treatment of the principles and practice of RNA virus research to ensure the widest possible audience. RNA Viruses: A Practical Approach will be of interest to all laboratories involved in virus culture, including both academic and industrial research groups and public health laboratories. | ||
| 590 | |a eBooks on EBSCOhost |b EBSCO eBook Subscription Academic Collection - Worldwide | ||
| 650 | 0 | |a RNA viruses |x Research |x Methodology. | |
| 650 | 0 | |a Viral genomes. | |
| 650 | 1 | 2 | |a RNA Viruses |x genetics |
| 650 | 1 | 2 | |a RNA Viruses |x isolation & purification |
| 650 | 2 | 2 | |a Genome, Viral |
| 650 | 6 | |a Virus à ARN |x Recherche |x Méthodologie. | |
| 650 | 6 | |a Génomes viraux. | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Biology. |2 bisacsh | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Microbiology. |2 bisacsh | |
| 650 | 7 | |a Viral genomes |2 fast | |
| 650 | 7 | |a RNS-Viren |2 gnd | |
| 650 | 7 | |a Virologie. |2 ram | |
| 650 | 7 | |a Virus à ARN. |2 ram | |
| 700 | 1 | |a Cann, Alan. | |
| 776 | 0 | 8 | |i Print version: |t RNA viruses. |d Oxford ; New York : Oxford University Press, 2000 |z 0199637172 |z 0199637164 |w (DLC) 99057577 |w (OCoLC)42849689 |
| 830 | 0 | |a Practical approach series ; |v 226. | |
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